Widespread injury of intestinal serosa with type I collagenase promote colonization of transplanted enteric neural stem cells

• Project/Area Number: 17K17008
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pediatric surgery
• Research Institution: Kanazawa Medical University
• Principal Investigator: YASUI Yoshitomo 金沢医科大学, 医学部, 助教 (10595325)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2019: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 細胞外マトリクス / 腸管神経細胞 / 腸管神経堤由来細胞 / 細胞外マトリックス / 発生・分化

Outline of Final Research Achievements

Hirschsprung’s disease (HSCR) causes abdominal distension and survival requires surgical excision of aganglionic bowel. However, after surgery, enterocolitis and fecal incontinence commonly occurs. Therefore, New strategies are needed to enhance bowel colonization by enteric neuron precursors and to reduce HSCR occurrence. In this study we demonstrated that injury of serosa with type I collagenase promote colonization of transplanted enteric neural crest derived Cells.

Academic Significance and Societal Importance of the Research Achievements

我々の研究結果は、I型コラゲナーゼを処理したレシピエントの大腸が、未治療の大腸よりも腸管神経系をより迅速に展開したことを示した。ドナーの神経幹細胞を受容可能な結腸にするこれらの発見は、細胞療法の現在の戦略を前進させることに役立つ。

Research Products

• [Journal Article] Target and Whirl Signs: Volvulus of Intussuscepted Colon in an Infant. (2020)
  • Author(s): Yasui Y, Shironomae T, Kuwahara T.
  • Journal Title: Clin Gastroenterol Hepatol. Volume: 18(5) Issue: 5 Pages: A34-A34
  • DOI: 10.1016/j.cgh.2019.01.033
  • Peer Reviewed
• [Journal Article] Collagen VI suppress fibronectin-induced enteric neural crest cell migration to downregulate of focal adhesion proteins. (2018)
  • Author(s): Nishida S, Yoshizaki H, Yasui Y, Kuwahara T, Kiyokawa E, Kohno M
  • Journal Title: Biochem Biophys Res Commun. Volume: 485 (1) Pages: 1461-1467
  • Peer Reviewed
• [Presentation] The role of extracellular matrix proteins in enteric neural crest cell migration (2019)
  • Author(s): Hisayoshi Yoshizaki, Shoichi Nishida, Yoshitomo Yasui, Tuyoshi Kuwahara and Miyuki Kohno
  • Organizer: The 13th World Congress of The International Cleft Lip and Palate Foundation, The 59th Annual Meeting of The Japanese Teratology Society
  • Int'l Joint Research
• [Presentation] VI型コラーゲンはフィブロネクチン誘導性の腸管神経細胞遊走を阻害する (2018)
  • Author(s): 吉崎尚良, 西田翔一, 安井良僚, 桑原強，清川悦子, 河野美幸
  • Organizer: 第91回日本生化学会大会
• [Presentation] VI型コラーゲンの腸管神経堤由来細胞移動に及ぼす影響 (2017)
  • Author(s): 吉崎尚良, 西田翔一, 安井良僚, 桑原強, 清川悦子, 河野美幸
  • Organizer: 2017年度生命科学系学会合同年次大会・第40回日本分子生物学会年会・第90回日本生化学会大会