Anti-thrumbin attenuates endothelial injury under endotoxemia
| Project/Area Number |
17K17048
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Emergency medicine
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| Research Institution | Gifu University |
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Principal Investigator |
OKAMOTO Haruka 岐阜大学, 医学部附属病院, 医員 (50585239)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 敗血症 / 血管内皮障害 / アンチトロンビン / リコンビナントアンチトロンビン / 血管内皮傷害 |
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| Outline of Final Research Achievements |
Anti-thrombin (AT) was recently reported to protect eGC from septic vasculitis but the underlying mechanism is still unknown.The aim The aim of the present study is to examine the structural basis and molecular mechanisms of AT effect on vascular endothelial injury in sepsis.Lipopolysaccharide (LPS) was intraperitoneally injected into 10-week-old male mice at a dose of 20 mg/kg and salaine or rAT was intraperitoneally injected 3 and 24 h after LPS injection. Using serum and/or lung tissue, we performed analysis on histology and ultrastructure. Survival rate of the AT-treated mice was significantly higher than that of the control mice 48 h after LPS injection (70% vs 24%, p<0.05). eGC injury in the heart, lung and liver capillaries was attenuated by AT treatment, as seen by thrombomodulin expression and ultrastructural analysis. AT treatment attenuated endothelial injury in multiple organ.
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| Academic Significance and Societal Importance of the Research Achievements |
グリコカリックスは細胞の恒常性維持に重要な役割を果たしており、その機能維持・回復は新しい治療の開発のターゲットとして注目されている。近年、ゲノム大量解析と同様に質量分析やグライコノミクスによる網羅的解析は盛んであるが、疾患の病因・病態からのアプローチは遅れている。敗血症を起因とする臓器障害には現在のところ対症療法しか方法がないため、新規治療の開発は社会的意義の大きい研究である
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Factors Enhancing Serum Syndecan-1 Concentrations: A Large-Scale Comprehensive Medical Examination2019
Author(s)
Oda K, Okada H, Suzuki A, Tomita H, Kobayashi R, Sumi K, Suzuki K, Takada C, Ishihara T, Suzuki K, Kano S, Kondo K, Iwashita Y, Yano H, Zaikokuji R, Sampei S, Fukuta T, Kitagawa Y, Okamoto H, Watanabe T, Kawaguchi T, Kojima T, Deguchi F, Miyazaki N, Yamada N, Doi T, Yoshida T et al.
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Journal Title
Journal of Clinical Medicine
Volume: 8
Issue: 9
Pages: 1320-1320
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Brain-Specific Ultrastructure of Capillary Endothelial Glycocalyx and Its Possible Contribution for Blood Brain Barrier2018
Author(s)
Ando Y, Okada H, Takemura G, Suzuki K, Takada C, Tomita H, Zaikokuji R, Hotta Y, Miyazaki N, Yano H, Muraki I, Kuroda A, Fukuda H, Kawasaki Y, Okamoto H, Kawaguchi T, Watanabe T, Doi T, Yoshida T, Ushikoshi H, Yoshida S, Ogura S.
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Journal Title
Scientific Reports
Volume: 8
Issue: 1
Pages: 17523-17523
DOI
Related Report
Peer Reviewed / Open Access
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