| Project/Area Number |
17K17214
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Dental engineering/Regenerative dentistry
|
|---|
| Research Institution | Nagasaki University |
|---|
Principal Investigator |
KAWAI Yosuke 長崎大学, 病院(歯学系), 助教 (50423629)
|
|---|
| Research Collaborator |
ASAHINA Izumi
KOMORI Toshihisa
SUMITA Yoshinori
SUGIHARA Takaki
|
|---|
| Project Period (FY) |
2017-04-01 – 2019-03-31
|
| Project Status |
Completed (Fiscal Year 2018)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | エクソソーム / 間葉系幹細胞 / 骨再生 |
|---|
| Outline of Final Research Achievements |
Bone marrow mesenchymal stem cell-derived exosomes were used to examine the effects on bone regeneration and bone differentiation. First, rat bone marrow-derived mesenchymal stem cells were cultured to isolate exosomes. Characterization of the isolated exosomes showed that the exosomes from the culture medium in which mesenchymal stem cells were induced for bone differentiation had increased expression of mRNA and microRNA that promotes bone differentiation, and when added to mesenchymal stem cells, it was found to promote osteoblast differentiation significantly compared with the non-addition group.
|
| Academic Significance and Societal Importance of the Research Achievements |
近年、骨粗鬆症治療薬としてビスフォスフォネート製剤の使用が増加している。この薬剤を内服している患者さんで歯科治療で抜歯などの外科的侵襲を加えると、顎骨壊死が引き起こされることが報告されている。高齢化に伴い顎骨壊死の患者数は増加傾向にあり、依然として確立された治療法は存在しない。この研究成果では細胞レベルでは骨髄由来間葉系幹細胞からのエクソソームは骨芽細胞の分化を促進する作用が示唆されており動物実験でこのことが証明できれば、顎骨壊死の発生機序の解明、副作用の回避を実現できる可能性が示唆され、学術的意義、社会的意義は大きいものと考える。
|
