| Project/Area Number |
17K17216
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
Sato Jun 北海道医療大学, 歯学部, 講師 (30624267)
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 歯胚形成 / エピジェネティクス |
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| Outline of Final Research Achievements |
In this study, we applied epigenetic modification to prepare odontogenic epithelial stem cells from Epithelial cell Rests of Malassez derived from the periodontal ligament, and investigated the ability to form tooth germs using the organ germ methods with mesenchymal stem cells.
First, odontogenic epithelial stem cells were prepared from Epithelial cell Rests of Malassez, and co-cultured with mesenchymal dental pulp cells containing dental pulp stem cells using the organ germ methods to prepare tooth germ-like cell mass. The expression of CD29 involved in tooth germ formation was analyzed. The percentage of CD29-positive cells was significantly higher.
The tooth germ-like cell mass was transplanted into the skull of the immunodeficient mouse, and the tooth germ forming ability was examined, but it could not be confirmed that hard tissue formation was induced from the transplanted tissue.
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| Academic Significance and Societal Importance of the Research Achievements |
これまで、歯胚を用いた歯の再生は研究が進められているが、エピジェネティクス修飾技術によって歯の再生が可能になれば、将来的には歯胚を用いることが無くても、親知らず等の抜去歯から採取可能な歯根膜マラッセ上皮細胞や歯髄細胞から、新たな歯胚を作製することが可能になるかもしれない。
本研究で得られた知見は、歯胚を用いること無く、患者自身の抜去歯とエピジェネティクス修飾技術を応用する方法によって新たな歯を再生するための基礎データとして意義あるものと考えられた。
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