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Possible role of p53 and CCN2 in osteocyte apoptosis under mechanical stress loading

Research Project

Project/Area Number 17K17303
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Orthodontics/Pediatric dentistry
Research InstitutionTohoku University

Principal Investigator

Yoshizawa Mitsuhiro  東北大学, 歯学研究科, 大学院非常勤講師 (40792164)

Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords歯学 / メカニカルストレス / 骨細胞 / アポトーシス / p53
Outline of Final Research Achievements

When a force is applied onto teeth in orthodontic treatment, bone remodeling is induced in the alveolar bone, leading to orthodontic tooth movement. Although osteocyte apoptosis might play an important role in regulating bone remodeling during orthodontic tooth movement, the underlying mechanisms of this phenomenon is not fully understood. We investigated the possible role of p53 and CCN2 in the osteocyte apoptosis induced by compressive force to murine parietal bones and osteocyte-like cell line, MLO-Y4. We found that compressive force increased p53 and CCN2 protein expressions in osteocytes in the murine parietal bones. In addition, osteocyte apoptosis, detected by TUNEL staining, was induced by compressive force in the murine parietal bones. Consistent with in vivo findings, compressive force-applied MLO-Y4 cells showed increased p53 and CCN2 expressions, and caspase-3 activity in vitro.

Academic Significance and Societal Importance of the Research Achievements

矯正歯科治療中の歯の移動は、矯正力が歯を介して歯槽骨に伝わり、歯槽骨内で骨リモデリングが誘導されることによって生じる。骨リモデリングは、破骨細胞による骨吸収と骨芽細胞による骨形成によって行われるが、矯正力による骨リモデリングは、骨細胞がメカノセンサーとしてメカニカルストレスを感知することよって制御されることが示唆されているが、骨細胞が骨基質内に存在するため、これまで骨細胞に関する研究は困難であった。本研究では、骨細胞の機能をin vivo、in vitro両方で検討し、骨細胞がメカニカルストレス下においてアポトーシスを誘導し、骨リモデリングに関与している可能性を示唆することができた。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (5 results)

All 2018 2017

All Journal Article (3 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (2 results)

  • [Journal Article] The Actin-Binding Protein PPP1r18 Regulates Maturation, Actin Organization, and Bone Resorption Activity of Osteoclasts2018

    • Author(s)
      Takuma Matsubara、Shoichiro Kokabu、Chihiro Nakatomi、Masayuki Kinbara、Toshihiro Maeda、Mitsuhiro Yoshizawa、Hisataka Yasuda、Teruko Takano-Yamamoto、Roland Baron、Eijiro Jimi
    • Journal Title

      Molecular and cellular biology

      Volume: 38 Issue: 4

    • DOI

      10.1128/mcb.00425-17

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Plectin, A Cytolinker Protein, Plays an Important Role in Differentiation and Actin Ring Formation in Osteoclasts2018

    • Author(s)
      Takuma Matsubara、Masayuki Kinbara、Toshihiro Maeda、Mitsuhiro Yoshizawa、Shoichiro Kokabu、Teruko Takano-Yamamoto
    • Journal Title

      Mathews Journal of Cytology and Histology

      Volume: 1 Pages: 006-006

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Regulation of osteoclast differentiation and actin ring formation by the cytolinker protein plectin.2017

    • Author(s)
      Matsubara T, Kinbara M, Maeda T, Yoshizawa M, Kokabu S, Takano Yamamoto T.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: Aug 5;489(4) Issue: 4 Pages: 472-476

    • DOI

      10.1016/j.bbrc.2017.05.174

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 歯科矯正用アンカースクリューを用いて水平的な咬合平面の傾斜を改善した正中離開症例2017

    • Author(s)
      吉澤 光弘、北浦英樹、福永智広、山本照子
    • Organizer
      第33回東北矯正歯科学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 歯科矯正用アンカースクリューを併用して上顎骨前方牽引を行った骨格性Ⅲ級症例2017

    • Author(s)
      吉澤光弘、福永智広、清流正弘、黒木毅、宮島悠旗、山本照子
    • Organizer
      第76回日本矯正歯科学会大会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2020-03-30  

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