Application to prevent and treat root absorption using naturally derived flavonoids and amelogenin peptide drug discovery.

• Project/Area Number: 17K17330
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Hiroshima University
• Principal Investigator: Yoshimi Yuki 広島大学, 病院(歯), 病院助教 (50707081)
• Research Collaborator: Kunimatsu Ryo ; Awada Tetsuya ; Kimura Aya
• Project Period (FY): 2017-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: アメロゲニン / 歯根吸収 / バイカリン / セメント芽細胞 / フラボノイド化合物 / フラボノイド

Outline of Final Research Achievements

In this study, we focused on the cementogenic effect of functional amelogenin peptide and the OPG expression effect of baicalin, which is a flavonoid compound. First, we examined the effects of amelogenin peptides and baicalin on periodontal tissue cells. We also verified the signal transduction pathway. Lastly, animal studies were conducted on the root resorption suppressing effect. As a result, it was suggested that amelogenin peptide and baicalin were effective in suppressing root resorption.

Academic Significance and Societal Importance of the Research Achievements

歯根吸収は、矯正歯科治療における副作用のうち、最も高頻度に認められるにも関わらず、発症機序の詳細は未だ解明されていない。歯根吸収のリスク因子は報告されているものの、現在のところ効果的な予防法は存在しない。そこに、安全で安定的なアメロゲニンペプチドを日常歯科臨床で行われている方法で投与する点や、健康補助食品としても注目されているフラボノイド類化合物を用いる簡便さが本研究の特徴である。歯の移動における機能性アメロゲニンペプチドおよびフラボノイド類化合物の作用機序を解明することは、歯根吸収の発症機序の解明や、歯周組織構成細胞間の相互作用の鶏鳴に貢献するため学術的に高い意義があると考えられる。

Research Products

• [Journal Article] Baicalin Promotes Osteogenic Differentiation of Human Cementoblast Lineage Cells Via the Wnt/β Catenin Signaling Pathway. (2018)
  • Author(s): Kimura A, Kunimatsu R, Yoshimi Y, Tsuka Y, Awada T, Horie K, Gunji H, Abe T, Nakajima K, Kitagawa M, Miyauchi M, Takata T, Tanimoto K.
  • Journal Title: Curr Pharm Des. Volume: 24 Issue: 33 Pages: 3980-3987
  • DOI: 10.2174/1381612824666181116103514
  • Peer Reviewed
• [Journal Article] The C-terminus of the amelogenin peptide influences the proliferation of human bone marrow mesenchymal stem cells. (2018)
  • Author(s): Kunimatsu R, Awada T, Yoshimi Y, Ando K, Hirose N, Tanne Y, Sumi K, Tanimoto K.
  • Journal Title: J Periodontol. Volume: 89 Issue: 4 Pages: 496-505
  • DOI: 10.1002/jper.17-0087
  • Peer Reviewed / Open Access
• [Journal Article] Effects of Human Full-length Amelogenin and C-terminal Amelogenin Peptide on the Proliferation of Human Mesenchymal Stem Cells Derived from Adipose Tissue. (2018)
  • Author(s): Ando K, Kunimatsu R, Awada T, Yoshimi Y, Tsuka Y, Sumi K, Horie K, Abe T, Nakajima K, Tanimoto K.
  • Journal Title: Curr Pharm Des. Volume: 24(25) Issue: 25 Pages: 2993-3001
  • DOI: 10.2174/1381612824666180816093227
  • Peer Reviewed
• [Journal Article] Effect of high-frequency near-infrared diode laser irradiation on periodontal tissues during experimental tooth movement in rats. (2018)
  • Author(s): Gunji H, Kunimatsu R, Tsuka Y, Yoshimi Y, Sumi K, Awada T, Nakajima K, Kimura A, Hiraki T, Hirose N, Yanoshita M, Tanimoto K
  • Journal Title: Lasers Surg Med. Volume: 印刷中 Issue: 7 Pages: 772-780
  • DOI: 10.1002/lsm.22797
  • Peer Reviewed / Open Access
• [Journal Article] Effects of high-frequency near-infrared diode laser irradiation on the proliferation and migration of mouse calvarial osteoblasts. (2018)
  • Author(s): Kunimatsu R, Gunji H, Tsuka Y, Yoshimi Y, Awada T, Sumi K, Nakajima K, Kimura A, Hiraki T, Abe T, Naoto H, Yanoshita M, Tanimoto K.
  • Journal Title: Lasers Med Sci. Volume: 印刷中 Issue: 5 Pages: 959-966
  • DOI: 10.1007/s10103-017-2426-0
  • Peer Reviewed / Open Access
• [Journal Article] Effects of C-terminal amelogenin peptides on the metabolism of osteoblasts. (2017)
  • Author(s): Tetsuya Awada, Ryo Kunimatsu* , Yuki Yoshimi, Naoto Hirose, Tomomi Mitsuyoshi, Keisuke Sumi, Kotaro Tanimoto
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 482(2) Issue: 4 Pages: 1154-1159
  • DOI: 10.1016/j.bbrc.2016.12.003
  • Peer Reviewed / Open Access
• [Presentation] バイカリンはWnt/βカテニンシグナル伝達機構を介してヒト培養セメント芽細胞分化を促進する (2018)
  • Author(s): 木村 綾, 國松 亮, 吉見 友希, 柄 優至, 粟田 哲也, 堀江 佳代, 郡司 秀美, 中島 健吾, 山田 桜, 平木 智香, 宮内 睦美, 高田 隆, 谷本 幸太郎
  • Organizer: 日本矯正歯科学会
• [Presentation] アメロゲニン由来新規ペプチドと骨髄由来間葉系幹細胞を用いた骨再生療法への展開 (2017)
  • Author(s): 粟田 哲也, 国松亮, 吉見友希, 廣瀬尚人, 安藤和代, 麻川由起, 柄優至, 平田伊佐雄, 加藤功一, 丹 根一夫, 谷本幸太郎
  • Organizer: 第 41 回日本口蓋裂学会総会・学術集会