Development of an applicable clinical kit which are useful for an indicator of the healthy periodontal tissue
Project/Area Number |
17K17384
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Social dentistry
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Research Institution | Nihon University |
Principal Investigator |
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Project Period (FY) |
2017-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | 歯周病 / 簡易キット / 口腔細菌 / 選択培地 / Rothia属 / 検査キット |
Outline of Final Research Achievements |
Recent some studies reported that Rothia dentocariosa (R.d) dominated the health-associated subgingival plaque communities. The aim in this study was to develop an easily applicable clinical kit for estimating the level of R.d in gingival crevicular fluid (GCF) samples. A clinical kit was developed using the selective medium broth made from an improved composition, i.e. addition of bromocresol purple, of the selective medium agar for the isolation of R.d. For examination of clinical efficacy, GCF samples were collected from periodontal healthy persons and periodontal patients. The result obtained by using this kit was compared with that of selective medium agar. The growth of oral bacteria other than R.d was markedly inhibited in this clinical kit. The results obtained by using this clinical kit were similar to that of selective medium agar. Since this clinical kit can accurately estimate the level of R.d in GCF samples, it will be useful for the clinical.
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Academic Significance and Societal Importance of the Research Achievements |
これまでの歯周病細菌検査のターゲットは、特にレッドコンプレックスと呼ばれる Porphyromonas gingivilis、Tannerella forsythia、Treponema denticolaを代表とする歯周病原菌の有無や菌量を調べるものが主流であるが、実際の歯周病の状態と一致しない場合も多く、臨床的意義に疑問を持つ声もある。このような面から、歯周病患者の細菌群と比較して健常者で顕著に認められる細菌を健康な歯周組織のバイオマーカーとして捉え、その変動を指標とすることにより、臨床における診断・治療・予後の判定、さらに歯周病のリスク判定に活用できると考えられる。
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Report
(3 results)
Research Products
(5 results)