Omics Profiles of Fecal Microbiota Change in Irritable Bowel Syndrome Patients with Diarrhea and Symptom Exacerbation

• Project/Area Number: 17K17584
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General internal medicine(including psychosomatic medicine); Gastroenterology
• Research Institution: Tohoku University
• Principal Investigator: Tanaka Yukari 東北大学, 医学系研究科, 大学院非常勤講師 (50721453)
• Project Period (FY): 2017-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2019: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 過敏性腸症候群 / 腸内細菌 / 口腔細菌 / 縦走オミクス / 腸内細菌叢 / 神経伝達物質 / メタボローム解析 / 細菌叢 / ストレス科学 / 下部消化管学

Outline of Final Research Achievements

Irritable bowel syndrome (IBS) is a disorder of gut-brain interaction. However, the role of gastrointestinal microbiota during IBS symptom exacerbation remains unclear. We tested the hypothesis that the fecal microbial composition are altered during the exacerbation of IBS symptoms. Fecal samples were collected from 43 men with IBS with diarrhea (IBS-D) and 40 healthy control (HC) men. Samples in the IBS-D patients were also collected during symptom exacerbation. The composition of the fecal microbiota was determined by analyzing the 16S rRNA gene and metabolites.
The fecal microbiota during IBS symptom exacerbation exhibited significant differences in the phylogenic pattern and short-chain fatty acid compared to without symptom exacerbation. Although there were no significant differences in the phylogenic pattern of fecal microbiota abundance between HCs and IBS-D patients.Our findings suggest that fecal microbiome are altered in IBS-D patients during exacerbation of symptoms.

Academic Significance and Societal Importance of the Research Achievements

今回、IBS患者と健常者間、更にIBSの通常排便時と腹部症状出現時比較により、消化管細菌叢のみならず、短鎖脂肪酸や神経伝達物質代謝の変化を明らかにした。これまで申請者らはIBS患者の大腸伸展刺激時の局所脳活動変化を報告してきた。IBSは中枢神経と消化管神経叢間での双方向の情報伝達関連が示唆されるものの、IBSの腹部症状出現タイミングの予測が困難なため研究手法が限られていた。本研究のように侵襲が少ない細菌叢をターゲットとし、同一被験者について複数回の検体採取、並びに縦走オミクス解析を行うことで、今後IBSの症状出現時の消化管環境メカニズムの解明に寄与できる可能性を示すことができた。

Research Products

• [Journal Article] 今月の主題 早期胃癌の範囲診断up to date 主題 早期胃癌の範囲診断-範囲診断困難例とその臨床的対応 潰瘍瘢痕併存胃癌 (2020)
  • Author(s): 齋藤 宏章、田中 由佳里、海野 修平、田中 一平、井上 薪、伊藤 聡司、友兼 正太郎、長南 明道、平澤 大、松田 知己、中堀 昌人、奥薗 徹、鈴木 憲次郎、阿部 洋子、五十嵐 公洋、名和田 義高
  • Journal Title: 胃と腸 Volume: 55 Issue: 1 Pages: 83-91
  • DOI: 10.11477/mf.1403201929
  • ISSN: 0536-2180, 1882-1219
  • Year and Date: 2020-01-25
  • Peer Reviewed
• [Journal Article] Cohort Profile: Tohoku Medical Megabank Project Birth and Three-Generation Cohort Study (TMM BirThree Cohort Study): rationale, progress and perspective. (2020)
  • Author(s): Kuriyama S, Metoki H, Kikuya M, Obara T, Ishikuro M, Yamanaka C, Nagai M, Matsubara H, Kobayashi T, 57名略, Yamamoto M.
  • Journal Title: Int J Epidemiol. Volume: 49(1) Issue: 1 Pages: 18-19
  • DOI: 10.1093/ije/dyz169
  • Peer Reviewed / Open Access
• [Journal Article] 今月の主題 上部消化管感染症-最近の話題を含めて 症例アトラス 感染性食道炎 ヘルペス食道炎 (2019)
  • Author(s): 田中 一平、名和田 義高、海野 修平、長南 明道、平澤 大、松田 知己、中堀 昌人、奥薗 徹、鈴木 憲次郎、阿部 洋子、田中 由佳里、五十嵐 公洋
  • Journal Title: 胃と腸 Volume: 54 Issue: 12 Pages: 1601-1604
  • DOI: 10.11477/mf.1403201875
  • ISSN: 0536-2180, 1882-1219
  • Year and Date: 2019-11-25
  • Peer Reviewed
• [Journal Article] 特集 脱･｢とりあえずCT｣!-スマートな腹痛診療 実践編 慢性的な(数カ月に及ぶ)下腹部痛 (2019)
  • Author(s): 田中 由佳里
  • Journal Title: medicina Volume: 56 Issue: 10 Pages: 1664-1666
  • DOI: 10.11477/mf.1402226504
  • ISSN: 0025-7699, 1882-1189
  • Year and Date: 2019-09-10
• [Journal Article] Relationship between sympathoadrenal and pituitary-adrenal response during colorectal distention in the presence of corticotropin-releasing hormone in patients with irritable bowel syndrome and healthy controls. (2018)
  • Author(s): Tanaka Y, Kanazawa M, Kano M, Tashiro M, Fukudo S.
  • Journal Title: PLoS One Volume: 13 Issue: 7 Pages: 1-16
  • DOI: 10.1371/journal.pone.0199698
  • Peer Reviewed / Open Access
• [Journal Article] Increased postprandial colonic motility and autonomic nervous system activity in patients with irritable bowel syndrome: A prospective study. (2018)
  • Author(s): Tanaka Y, Kanazawa M, Palsson OS, Van Tilburg MA, Gangarosa LM, Fukudo S, Drossman DA, Whitehead WE.
  • Journal Title: J Neurogastroenterol Motil Volume: 24 Issue: 1 Pages: 87-95
  • DOI: 10.5056/jnm16216
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Altered brain and gut responses to corticotropin-releasing hormone (CRH) in patients with irritable bowel syndrome. (2017)
  • Author(s): Kano M, Muratsubaki T, Van Oudenhove L, Morishita J, Yoshizawa M, Kohno K, Yagihashi M, Tanaka Y, Mugikura S, Dupont P, Ly HG, Takase K, Kanazawa M, Fukudo S.
  • Journal Title: Sci Rep Volume: 29 Issue: 1 Pages: 12425-12425
  • DOI: 10.1038/s41598-017-09635-x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 過敏性腸症候群の症状出現時の口腔―腸内細菌叢ならびに代謝物の変化 (2019)
  • Author(s): 田中 由佳里
  • Organizer: 第92回日本内分泌学会総会
• [Presentation] ITを駆使したキャリア・ライフバランスの構築へ向けて (2019)
  • Author(s): 田中 由佳里
  • Organizer: 第56回日本小児外科学会学術総会
  • Invited
• [Presentation] 過敏性腸症候群の症状出現時の口腔―腸内細菌叢の変化 (2018)
  • Author(s): 田中由佳里
  • Organizer: JDDW
• [Presentation] Changes in Oral and Fecal Microbiota during the Exacerbation of Symptoms Accompanying Irritable Bowel Syndrome with Diarrhea (2018)
  • Author(s): Yukari Tanaka
  • Organizer: 3rd Meeting of the Federation of Neurogastroenterology and Motility
  • Int'l Joint Research
• [Presentation] Searching for biological factors in a biopsychosocial model of irritable bowel syndrome (2017)
  • Author(s): Yukari Tanaka
  • Organizer: UEGW2018 (欧州消化器病学会）
  • Int'l Joint Research / Invited