| Project/Area Number |
17K17595
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
Cell biology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 極性 / オーキシン / PIN / 細胞膜 / 流動性 |
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| Outline of Final Research Achievements |
My previous research identified the formation of dot-like clusters of PIN proteins at PMs. It also identified that PIN clusters are established or maintained by the cell wall components and the unknown function protein MAB4. Here I analyzed the role of MAB4 and cell wall in PIN cluster formation. I also performed forward genetic and chemical genetic screening to further elucidate the mechanism of PIN cluster formation.
I identified that MAB4 and PIN proteins both formed cluster-like structures at PMs. I also identified that they are largely colocalized. These findings suggested that MAB4 directly regulate PIN cluster formation. In contrast, I could not get clear evidence that demonstrate the direct involvement of cell wall in PIN cluster formation. Regarding the screening, I identified that phospholipids composition as well as the ATP biosynthesis play important roles for PIN cluster formation. I also succeeded to isolate one mutant that display defects in PIN cluster formation.
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| Academic Significance and Societal Importance of the Research Achievements |
オーキシンの極性輸送の減少はダーウィン親子により140年前に見出されていたが，その分子機構は未だ未知なままである．本研究の成果は長らく未知とされてきたオーキシンの極性輸送の分子機構を明らかにするものであり，生物学上重要な研究課題である．また，オーキシンは単為結果や除草剤に利用されていることから，本研究の進展は将来的な農業利用の方策の礎となるものである．
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