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Search for functional ingredients and foods altering cholesterol-bile acid metabolism for prevention of NASH development

Research Project

Project/Area Number 17K17883
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Eating habits
Applied health science
Research InstitutionThe University of Tokushima

Principal Investigator

SHIMIZU Mayuko  徳島大学, 大学院医歯薬学研究部(医学域), 助教 (20759021)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords非アルコール性脂肪肝炎 / NASH / コレステロール / マクロファージ / 肝線維化 / 肝線維化モデル動物 / オウバク / ベルベリン / 生薬 / 胆汁酸 / 食品機能 / モデル動物 / 栄養生理学 / 肝臓学 / 機能性食品 / 食事療法
Outline of Final Research Achievements

We investigated the preventive effect of Phellodendron bark (PB) and its component berberine against non-alcoholic steatohepatitis (NASH). To test this, mice were fed a high-fat and high-cholesterol (HFC) diet which was containing 5% PB or 0.06% berberine powder. Histological steatohepatitis including steatosis, lobular inflammation and fibrosis (stage 1 or 2) were seen in mice fed the HFC diet. Administration of PB and berberine attenuated steatosis and lobular inflammation with decreasing cholesterol content in the liver. Histologically, Mac-2 positive-stained multinuclear giant cells, that is macrophages, were frequently observed in the HFC-diet fed mice, but they were rarely seen in the berberine-fed mice. Liver fibrosis, which is an advanced condition of NASH, was significantly suppressed in the berberine-fed mice. It was suggested that berberine may suppress hepatic inflammation and fibrosis through its action on macrophages during NASH development.

Academic Significance and Societal Importance of the Research Achievements

本研究ではオウバクおよびその主成分ベルベリンの摂取がNASHおよび肝線維化の程度を抑制する可能性とその作用機序の一端を示した。NASHやその進展病態である肝線維化に対する特効薬は未だ開発途上であるが、本研究の成果はNASHおよび肝線維化の食事療法や治療薬の開発の基礎的なエビデンスになると考えられる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (8 results)

All 2020 2019 2018

All Presentation (8 results) (of which Int'l Joint Research: 4 results)

  • [Presentation] Phellodendron bark and its component berberine prevent nonalcoholic steatohepatitis-related fibrosis in mice2020

    • Author(s)
      Mayuko Ichimura-Shimizu, Koichi Tsuneyama
    • Organizer
      APASL 2020 (The Asian Pacific Association for the Study of the Liver)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] NASH線維化肝におけるpathogen lipidsの同定2020

    • Author(s)
      市村真祐子、平修、松本穣、尾矢剛志、小川博久、常山幸一
    • Organizer
      徳島医学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Fibrogenesis of nonalcoholic steatohepatitis begins around cholesterol-laden macrophage in the experimental mouse model2019

    • Author(s)
      Mayuko Ichimura, Takeshi Oya, Koichi Tsuneyama
    • Organizer
      UEG week 2019 (UEG:United European Gastroenterology)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 非アルコール性脂肪肝炎モデルマウスにおける肝線維化発症とコレステロール貪食マクロファージの関連2019

    • Author(s)
      市村真祐子、尾矢剛志、小川博久、大曲勝久、常山幸一
    • Organizer
      日本病理学会総会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Fibrosis begins around lipid-phagocytic macrophages in high fat/cholesterol/cholate diet-induced nonalcoholic steatohepatitis model2019

    • Author(s)
      Mayuko Ichimura, Koichi Tsuneyama
    • Organizer
      Liver meeting(AASLD:American Association for Study of Liver Diseases)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Establishment of a novel dietary-induced mouse model showing steatohepatitis with severe fibrosis2019

    • Author(s)
      Mayuko Ichimura, Hirohisa Ogawa, Minoru Matsumoto, Koichi Tsuneyama
    • Organizer
      UEG week 2019 (UEG:United European Gastroenterology)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] オウバク成分は非アルコール性脂肪肝疾患(NAFLD)改善に関与するか?,2018

    • Author(s)
      天野 久実, 中川 友希江, 市村 真祐子, 辻 愛, 北岸 靖子, 松田 覚
    • Organizer
      第91回日本生化学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 脂肪性肝障害に対する少量アセトアルデヒド摂取の影響2018

    • Author(s)
      中川 友希江, 天野 久実, 市村 真祐子, 辻 愛, 北岸 靖子, 松田 覚
    • Organizer
      第91回日本生化学会大会
    • Related Report
      2018 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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