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Analysis of degradation mechanism of crystalline carbohydrates by high speed and precision single molecule observation

Research Project

Project/Area Number 17K18429
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Environmental agriculture(including landscape science)
Biophysics
Research InstitutionInstitute for Molecular Science (2018)
Okazaki Research Facilities, National Institutes of Natural Sciences (2017)

Principal Investigator

Nakamura Akihiko  分子科学研究所, 生命・錯体分子科学研究領域, 助教 (20752968)

Research Collaborator Okazaki Kei-ichi  
Iino Ryota  
Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsキチナーゼ / セルラーゼ / バイオマス / 1分子計測 / プロセッシブ運動 / 一分子観察 / キチン / セルロース / 糖質加水分解酵素
Outline of Final Research Achievements

Chitin hydrolase (Chitinase) degrade the chitin crystal which is the main component of outer shell of insects or crabs. Chitinase which has a good activity to crystalline chitin moves on the surface, but moving mechanism is still unknown. In this research, we revealed that chitinase move forward due to the destabilization of backward state by the hydrolysis of molecular chain. Our findings will be helpful to design the better mutants.

Academic Significance and Societal Importance of the Research Achievements

多くの運動する酵素は細胞内でATPなどの高エネルギー化学物質をエネルギー源として運動することが知られている。しかし今回の研究で、キチン加水分解酵素は細胞外で長いキチン分子鎖を短い2糖単位に切断し可溶化する際の変化で運動していることが明らかとなった。これは分子の運動メカニズムの解明に非常に有意義な成果である。また運動機構を考慮するとキチン分解酵素での反応で生成物を取り除くことが効率的な分解に重要であると考えられ、分解反応系の効率化にも有意義な結果が得られた。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (13 results)

All 2018 2017

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (9 results) (of which Int'l Joint Research: 4 results,  Invited: 7 results)

  • [Journal Article] Rate constants, processivity, and productive binding ratio of chitinase A revealed by single- molecule analysis2018

    • Author(s)
      Akihiko Nakamura, Tomoyuki Tasaki, Yasuko Okuni, Chihong Song, Kazuyoshi Murata, Toshiya Kozai, Mayu Hara, Hayuki Sugimoto, Kazushi Suzuki, Takeshi Watanabe, Takayuki Uchihashi, Hiroyuki Noji and Ryota Iino
    • Journal Title

      Phys. Chem. Chem. Phys.

      Volume: 20 Issue: 5 Pages: 3010-3018

    • DOI

      10.1039/c7cp04606e

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Processive chitinase is Brownian monorail operated by fast catalysis after peeling rail from crystalline chitin2018

    • Author(s)
      Nakamura Akihiko、Okazaki Kei-ichi、Furuta Tadaomi、Sakurai Minoru、Iino Ryota
    • Journal Title

      Nature Communications

      Volume: 9 Issue: 1 Pages: 3814-3814

    • DOI

      10.1038/s41467-018-06362-3

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Single-molecule imaging and manipulation of biomolecular machines and systems2018

    • Author(s)
      Iino Ryota、Iida Tatsuya、Nakamura Akihiko、Saita Ei-ichiro、You Huijuan、Sako Yasushi
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - General Subjects

      Volume: 1862 Issue: 2 Pages: 241-252

    • DOI

      10.1016/j.bbagen.2017.08.008

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Visualization of functional structure and kinetic dynamics of cellulases2018

    • Author(s)
      Akihiko Nakamura, Ryota Iino
    • Journal Title

      Glycobiophysics

      Volume: 印刷中

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] キチン加水分解酵素は ATP を使わずに運動するブラウニアンラチェットである2018

    • Author(s)
      中村 彰彦
    • Organizer
      分子モーター討論会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] rocessive chitinase is Brownian ratchet moving unidirectionally by fast catalysis2018

    • Author(s)
      Akihiko Nakamura
    • Organizer
      Sendai Molecular Motor Meeting 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] キチン加水分解酵素は速い加水分解でブラウン運動を制御する2018

    • Author(s)
      中村 彰彦
    • Organizer
      多糖の未来フォーラム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Direct observation of 1 nm steps of a “burnt‐bridge” Brownian ratchet SmChiA on crystalline chitin with gold nanoprobe2018

    • Author(s)
      Akihiko Nakamura
    • Organizer
      The 79th Okazaki Conference Synthetic, Biological, and Hybrid Molecular Engines
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Resolving 1 nm steps of a Brownian-ratchet chitinase with gold nano particle2018

    • Author(s)
      Akihiko Nakamura
    • Organizer
      International Symposium on Molecular Science - Physical Chemistry / Theoretical Chemistry, Chemoinformatics, Computational Chemistry - Cosponsored by Japan Society for Molecular Science
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 1 分子解析によりセルラーゼ/キチナーゼの何が見えるのか2017

    • Author(s)
      中村彰彦
    • Organizer
      セルラーゼ研究会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Direct Observation of 1 nm Steps of Chitinase A Molecules with Gold Nano Probes2017

    • Author(s)
      Akihiko Nakamura, Ryota Iino
    • Organizer
      Gordon Research Conference Cellulases & Other Carbohydrate-Active Enzymes
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 霊菌Serratia marcescens由来キチナーゼSmChiAの運動律速段階の解明2017

    • Author(s)
      中村彰彦、飯野亮太
    • Organizer
      日本応用糖質学会
    • Related Report
      2017 Research-status Report
  • [Presentation] One nanometer steps and the rate-limiting step of Serratia marcescens chitinase A resolved by gold nanoprobe2017

    • Author(s)
      Akihiko Nakamura, Ryota Iino
    • Organizer
      日本生物物理学会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2020-03-30  

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