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Experiments on the quantification of protein in trans-boundary suspended particulate matters

Research Project

Project/Area Number 17K18811
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Research Field Earth and planetary science and related fields
Research InstitutionPrefectural University of Kumamoto

Principal Investigator

Zhang Daizhou  熊本県立大学, 環境共生学部, 教授 (90322726)

Co-Investigator(Kenkyū-buntansha) 松崎 弘美  熊本県立大学, 環境共生学部, 教授 (30326491)
山元 涼子  熊本県立大学, 環境共生学部, 助手 (50580429)
Research Collaborator Naganuma Ayumi  
Hashimoto Yuki  
Project Period (FY) 2017-06-30 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2018: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2017: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Keywords越境大気汚染 / 浮遊粒子状物質 / タンパク質 / 定量分析 / 東アジア
Outline of Final Research Achievements


Based on the traditional protein quantification technique of BCA, we developed in laboratory an approach to quantify the protein in airborne particulate matters. Tests for its application to the real atmosphere showed its effectiveness to depict the variation of airborne protein concentration with the changes of weather and the evolution of air pollutants concentration.
With the developed method, we tried to quantify the airborne protein in trans-boundary-moved air parcels. Results show that the concentration of protein in the air parcels varied in a large range of hundreds to thousands of nanograms of protein per cubic meter, responding to various weather and pollutant conditions. When Asian dust appeared, the range was from several to more than 10 micrograms of proteins per cubic meter, much larger than under other weather or pollutant conditions.

Academic Significance and Societal Importance of the Research Achievements

大気汚染による健康影響の原因のひとつ物質は,浮遊粒子状物質中のタンパク質である.本研究では,複数分野の知識と技術を融合して,濃度が極めて低い大気浮遊粒子状物質中のタンパク質について天気と大気汚染の変化時間スケールの変動を求める方法を開発した.開発した方法を用いて,さまざまな天気及び大気汚染の状況の下で,浮遊粒子状物質中のタンパク質の定量が可能となった.そのような高時間分可能のデータは,知っている限りまだ報告されたことがない.今後の応用によって,浮遊粒子状物質の健康影響の評価,および大気中の生物物質の生態系へのかかわりの研究推進に独創的かつ先駆的な貢献が期待できる.

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (5 results)

All 2018 Other

All Presentation (3 results) Remarks (2 results)

  • [Presentation] 大気浮遊粒子中のタンパク質抽出法の検証2018

    • Author(s)
      1橋本優希,長沼歩,張代洲,小島知子
    • Organizer
      第59回大気環境学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 大気浮遊粒子中のタンパク質抽出法の検証2018

    • Author(s)
      橋本優希,長沼歩,張代洲,小島知子
    • Organizer
      第35回エアロゾル科学・技術研究討論会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Concentration and Viability of Bacterial Aerosols Associated with Weather in Asian Continental Out-flow: Summary of Recent Studies at PUK2018

    • Author(s)
      Daizhou Zhang (張代洲)
    • Organizer
      第11回大気バイオエアロゾルシンポジウム
    • Related Report
      2018 Annual Research Report
  • [Remarks] News

    • URL

      http://www.pu-kumamoto.ac.jp/~aerl/

    • Related Report
      2018 Annual Research Report
  • [Remarks] 本研究における観測活動、国際交流、学会発表は随時に知らせている.

    • Related Report
      2018 Annual Research Report

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Published: 2017-07-21   Modified: 2020-03-30  

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