Construction of a system to attain affinity maturation of anti-membrane protein antibodies
Project/Area Number |
17K19007
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Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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Allocation Type | Multi-year Fund |
Research Field |
Chemical engineering and related fields
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Research Institution | The University of Tokyo |
Principal Investigator |
Kawahara Masahiro 東京大学, 大学院工学系研究科(工学部), 准教授 (50345097)
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Project Period (FY) |
2017-06-30 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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Keywords | シグナル伝達 / 抗体 / 膜蛋白質 / スクリーニング / 親和性成熟 |
Outline of Final Research Achievements |
Membrane proteins are important targets in medicine. Thus, it is necessary to develop a system to obtain antibodies with high specificity and high affinity against membrane proteins in rapid, easy, and reliable ways. In this study we developed a system to rapidly and easily select specific antibodies against a target membrane protein antigen that is displayed on the mammalian cell surface. This system also allowed easy acquisition of antibodies with higher affinity against a target antigen.
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Academic Significance and Societal Importance of the Research Achievements |
本研究では、既往の抗膜蛋白質抗体選択法の問題点を克服した上で、抗体の親和性成熟を合理的に達成可能なユニークな系の開発に成功しており、学術的に意義深い。本研究の手法を用いて、種々の病態関連膜蛋白質に対する抗体が将来的に取得できれば、抗体医薬品としての臨床応用も含めた大きな社会的波及成果が期待できる。
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Report
(3 results)
Research Products
(19 results)
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[Journal Article] Microfluidic preparation of anchored cell membrane sheets for in vitro analyses and manipulation of the cytoplasmic face2017
Author(s)
Izuta,S., Yamaguchi,S., Misawa,R., Yamahira,S., Tan,M., Kawahara,M., Suzuki,T., Takagi,T., Sato,K., Nakamura,M., Nagamune,T. and Okamoto,A.
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Journal Title
Sci. Rep.
Volume: 7:14962
Issue: 1
Pages: 14962-14962
DOI
Related Report
Peer Reviewed / Open Access
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