| Project/Area Number |
17K19030
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nano/Micro science and related fields
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2017-06-30 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | ポリマーベシクル / 高分子ベシクル / 核酸医薬 / DDS / グライコベシクル / 分子透過能 / 核酸合成 / 酵素反応場 |
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| Outline of Final Research Achievements |
In this study, we synthesized nucleic acid medicine, which is attracting attention as a next-generation drug, by a rolling circle amplification in molecular permeable polymer vesicles that we developed previously. In the course of conducting this study, we also found that amphiphilic block polymer composed of carbohydrate and poly (n-propyl oxazoline), which shows a lower critical solution temperature around room temperature, self-assembled into molecular permeable polymer vesicles. The introduction of hydrophobic polymers showing LCST-like behavior such as poly(n-propyl oxazoline)s as a hydrophobic segment represents a new approach to constructing permeable polymer vesicles. Our study thus opens up an avenue for designing and controlling the molecular permeability of polymer vesicles.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は、我々が独自に開発した分子透過を示す高分子ベシクルの性質を生かし、高分子ベシクル内での酵素反応により核酸医薬産生を行なった。これにより、従来の核酸送達キャリアが持つ封入率、毒性及び安定性に関する問題を克服する足がかりになると考えられる。また、水和能を有するpoly(n-propyl oxazoline)が、ポリマー二分子膜の分子透過性を向上させることが明らかとなり、分子透過能性高分子ベシクルの設計指針に結びつくと考えられる。
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