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Development of innovative manipulation technology for membrane proteins

Research Project

Project/Area Number 17K19211
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Research Field Biomolecular chemistry and related fields
Research InstitutionKansai University

Principal Investigator

Kuzuya Akinori  関西大学, 化学生命工学部, 教授 (00456154)

Project Period (FY) 2017-06-30 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
KeywordsDNAオリガミ / DNAナノテクノロジー / 脂質2重膜 / ナノディスク / 膜タンパク / 金ナノ粒子 / 分子ロボティクス / ナノマシン
Outline of Final Research Achievements

A square-shape DNA origami motif with wide inner cavity for encapsulation of lipid bilayers has been designed and prepared. Atomic force microscopy (AFM) showed successful formation of the desired nanostructures. Selective and efficient incorporation of gold nanoparticles applying solution-freezing technique, and real-time and single-molecule observation of CyD rotaxane attached to DNA origami nanostructures using high-speed AFM were also successful.

Academic Significance and Societal Importance of the Research Achievements

本研究で設計した口の字型DNAオリガミ構造体により、「人工ナノディスク」の構築への端緒が開かれた。また、近年注目が集まりつつある分子機械について、ほぼ世界で初めて高速AFMによるリアルタイム観察を行うことに成功した。生体分子との組み合わせにより、AFMでは観察が難しい小分子でもその存在を可視化できることを示すとともに、観察基盤としてのDNAオリガミ構造体の有用性も示すことができた。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (22 results)

All 2019 2018 2017 Other

All Int'l Joint Research (7 results) Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (8 results) (of which Int'l Joint Research: 8 results,  Invited: 5 results) Book (1 results) Remarks (3 results)

  • [Int'l Joint Research] スイス連邦工科大学チューリッヒ校(スイス)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] マレーシア科学大学(マレーシア)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] ドレスデン工科大学(ドイツ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] ヤギエヴォ大学(ポーランド)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] ETH Zurich(Switzerland)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] Jagiellonian University(Poland)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] Universiti Sains Malaysia(Malaysia)

    • Related Report
      2017 Research-status Report
  • [Journal Article] Artificial Smooth Muscle Model Composed of Hierarchically Ordered Microtubule Asters Mediated by DNA Origami Nanostructures2019

    • Author(s)
      Matsuda Kento、Kabir Arif Md. Rashedul、Akamatsu Naohide、Saito Ai、Ishikawa Shumpei、Matsuyama Tsuyoshi、Ditzer Oliver、Islam Md. Sirajul、Ohya Yuichi、Sada Kazuki、Konagaya Akihiko、Kuzuya Akinori、Kakugo Akira
    • Journal Title

      Nano Letters

      Volume: 19 Issue: 6 Pages: 3933-3938

    • DOI

      10.1021/acs.nanolett.9b01201

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Bulk pH-Responsive DNA Quadruplex Hydrogels Prepared by Liquid- Phase, Large-Scale DNA Synthesis2018

    • Author(s)
      Shizuma Tanaka, Shinsuke Yukami, Kazuki Fukushima, Kenta Wakabayashi, Yuichi Ohya and Akinori Kuzuya
    • Journal Title

      ACS Macro Letters

      Volume: 7 Issue: 3 Pages: 295-299

    • DOI

      10.1021/acsmacrolett.8b00063

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Hydrogels Utilizing G-Quadruplexes2017

    • Author(s)
      Akinori Kuzuya and Shizuma Tanaka
    • Journal Title

      MOJ Polymer Science

      Volume: 1 Issue: 6 Pages: 00033-00033

    • DOI

      10.15406/mojps.2017.01.00033

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Real-Time Observation of The Movements of DNA Origami Pinching Devices on Mica Using High-Speed AFM2019

    • Author(s)
      Akinori Kuzuya
    • Organizer
      16th Annual Conference on Foundations of Nanoscience (FNANO 2019)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Single-Molecule Observation of alpha-CyD Rotaxane Incorporated into DNA Origami with Nanocavities2018

    • Author(s)
      Akinori Kuzuya, Naohide Akamatsu, Mana Ishino, Yuichi Ohya
    • Organizer
      15th Annual Conference on Foundations of Nanoscience (FNANO 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Single-Molecule Observation of α-CyD Rotaxane Incorporated into DNA Origami with Nanocavities2018

    • Author(s)
      Akinori Kuzuya, Naohide Akamatsu, Mana Ishino, Yuichi Ohya
    • Organizer
      The 13th International Symposium in Science and Technology at Cheng Shiu University 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] DNA Quadruplex Hydrogels: An Application of Synthetic DNA as Bulk Material2018

    • Author(s)
      Akinori Kuzuya
    • Organizer
      First International Conference on 4D Materials and Systems
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Single-Molecule Observation of alpha-CyD Rotaxane Incorporated into DNA Origami with Nanocavities2018

    • Author(s)
      Akinori Kuzuya
    • Organizer
      BIONANO2018 Workshop
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Single-Molecule and Real-Time Observation of Cyclodextrin-Rotaxane Incorporated into DNA Origami with Nanocavities2018

    • Author(s)
      Akinori Kuzuya, Naohide Akamatsu, Mana Ishino, Hitomi Okuyama, Fumito Baba, Yuichi Ohya
    • Organizer
      The 45th International Symposium on Nucleic Acids Chemistry (ISNAC2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Single-Molecule Observation of alpha-CyD Rotaxane Incorporated into DNA Origami with Nanocavities2018

    • Author(s)
      Akinori Kuzuya, Naohide Akamatsu, Mana Ishino, and Yuichi Ohya
    • Organizer
      15th Annual Conference on FOUNDATIONS OF NANOSCIENCE: SELF-ASSEMBLED ARCHITECTURES AND DEVICES (FNANO18)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] PEG Hydrogels Utilizing DNA Quadruplexes as Crosslinking Points2018

    • Author(s)
      Akinori Kuzuya
    • Organizer
      INTERNATIONAL CONGRESS ON PURE & APPLIED CHEMISTRY (ICPAC) 2018
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Book] 分子ロボティクス概論2019

    • Author(s)
      分子ロボティクス研究会(分担執筆)
    • Total Pages
      301
    • Publisher
      CBI学会出版
    • ISBN
      9784990907648
    • Related Report
      2018 Annual Research Report
  • [Remarks] 関西大学 知能分子学研究室

    • URL

      http://wps.itc.kansai-u.ac.jp/mol-mach/

    • Related Report
      2018 Annual Research Report
  • [Remarks] 関西大学 化学生命工学部 知能分子学研究室

    • URL

      http://wps.itc.kansai-u.ac.jp/mol-mach/

    • Related Report
      2017 Research-status Report
  • [Remarks] 関西大学 学術情報システム

    • URL

      http://gakujo.kansai-u.ac.jp/profile/ja/e43atXUd5P3aff3deIc240Ib6.html

    • Related Report
      2017 Research-status Report

URL: 

Published: 2017-07-21   Modified: 2020-03-30  

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