| Budget Amount *help |
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
In my research human keratinocyte differentiation have been studied using three-dimensional culture system, where air exposure is essential for keratinization with multilayer. In order to clarify the mechanism by which air-exposure causes differentiation, search for mRNAs and proteins which might cause cellular signaling on the initiation of differentiation. By comparison with mRNAs from day 2-cultrue (air and liquid), several candidates of specific key molecules were identified and their characterization is ongoing.
In paralleled with this approach, the amniotic fluid from mouse uterus were investigated for their component by mass spectrometric analysis and its ability to promote differentiation. Skin formation during several fetal days were analyzed for barrier function as well as transglutaminase activity. Partial purification is underway based on the promoting activity for keratinocyte differentiation using several marker proteins.
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