Novel mycovirus transmission pathway via conidia in filamentous fungi
Project/Area Number |
17K19266
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Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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Allocation Type | Multi-year Fund |
Research Field |
Agricultural and Environmental Biology and related fields
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Research Institution | Kobe University |
Principal Investigator |
Ikeda Kenichi 神戸大学, 農学研究科, 准教授 (40437504)
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Project Period (FY) |
2017-06-30 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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Keywords | ヴァイロコントロール / 菌類ウイルス / 細胞質不和合性 / 菌糸融合 / 分生子形成 / マイコウイルス / 菌類学 |
Outline of Final Research Achievements |
Virocontrol is a novel disease protection method by using mycoviruses. The major obstacle is heterogenic incompatibility system that blocks to transmit mycovirus to arbitrary fungal strains. I found a mutant that was able to transmit mycovirus in Rosellinia necatrix. In this study, we tried to elucidate the characters of the mutant. I found that the mutant produce conidia during plate culture suggesting that mycovirus is transmitted via conidia. I observed that the conidia of the mutant anastomosed to vegetative hypha that was different genetic back ground. I also analyzed genome sequences and expression profiles by next generation sequence. I found numerous genomic mutation points and differential expression genes.
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Academic Significance and Societal Importance of the Research Achievements |
菌類ウイルスは糸状菌病害の病原性を低下させる作用を有しており、ウイルスを利用した生物防除剤としての利用が期待される。この防除法をヴァイロコントロールと呼んでいる。しかしながら、任意菌株へ効率よく伝搬する方法が求められていた。研究過程で見出された変異菌は任意菌株へウイルスを伝搬することができた。この変異菌は分生子を形成する特徴を有しており、分生子がウイルス伝搬に関わっていることが考えられた。この仕組みを明らかとすることで、任意菌株へのウイルス移行が容易となり、ヴァイロコントロールの実用化につながる。
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Report
(3 results)
Research Products
(14 results)
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[Journal Article] Ralfuranones contribute to mushroom-type biofilm formation by Ralstonia solanacearum strain OE1-1.2018
Author(s)
Mori, Y., Hosoi, Y., Ishikawa, S., Hayashi, K., Asai, Y., Ohnishi, H., Shimatani, M., Inoue, K., Ikeda, K., Nakayashiki, H., Nishimura, Y., Ohnishi K., Kiba, A., Kai, K., Hikichi, Y.
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Journal Title
Molecular Plant Pathology
Volume: 19
Issue: 4
Pages: 975-985
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] A fungal argonaute interferes with RNA interference.2018
Author(s)
Nguyen, Q., Iritani, A., Ohkita, S., Vu, B.V., Yokoya, K., Matsubara, A., Ikeda, K., Suzuki, N., Nakayashiki, H.
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Journal Title
Nucleic Acids Research
Volume: 46
Issue: 5
Pages: 2495-2508
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Acidic soil conditions suppress zoospore release from zoosporangia in Olpidium virulentus.2017
Author(s)
Iwamoto, Y., Inoue, K., Nishiguchi, S., Matsuura, K., Aino, M., Nakayashiki, H., Ikeda, K.
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Journal Title
Journal of General Plant Pathology
Volume: 83
Issue: 4
Pages: 240-243
DOI
Related Report
Peer Reviewed
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