| Project/Area Number |
17K19348
|
|---|
| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Molecular and Genome biology and related fields
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2017-06-30 – 2019-03-31
|
| Project Status |
Completed (Fiscal Year 2018)
|
| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2018: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
|
|---|
| Keywords | 染色体構造構築 |
|---|
| Outline of Final Research Achievements |
This study aimed to identify self-assembly activity of mitotic chromosomes by using Xenopus egg extract and human somatic cells. First, proteinaceous factors specifically bound to self-assembled mitotic chromosomes were systematically detected by mass-spectrometry, and several non-histone DNA binding proteins were identified. Among them, poly (ADP-ribose) polymerase 1 (PARP-1) was tested for function in mitotic chromosome assembly and we found that PARP-1 is required for chromosome condensation rather than self-assembly of chromosomes. Though the deficient condensation could prevent chromosome disassembly, we could reveal a novel function of PARP-1 in mitotic chromosome condensation.
|
| Academic Significance and Societal Importance of the Research Achievements |
本研究では、新しい細胞生物学の概念として、染色体自己集合活性の実体を同定することを目指した。染色体自己集合活性はこれまで存在こそ示唆されていたものの、分子的な実証がなされていなかったが、本研究において、複数の染色体自己集合活性候補因子の同定に成功した。それらすべての具体的な分子機能の理解には至っていないが、候補因子の一つであるPARP-1の、分裂期染色体形成における新たな役割を明らかにできた。今後の研究でその他の因子の解析が進めば、染色体自己集合活性のみならず、染色体凝縮機構も含めた新しい分子ネットワークを描くことができ、そのシーズを作成できた本研究の学術的意義は大きい。
|
