The role of Pax6 in spermatogenesis and determination of sex bias in the mouse
Project/Area Number |
17K19372
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Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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Allocation Type | Multi-year Fund |
Research Field |
Biology of Cells to Organisms, and related fields
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Research Institution | Tohoku University |
Principal Investigator |
Osumi Noriko 東北大学, 医学系研究科, 教授 (00220343)
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Co-Investigator(Kenkyū-buntansha) |
木村 龍一 東北大学, 医学系研究科, 助教 (00781759)
|
Research Collaborator |
Jasper Germeraad
TATEHANA Misako
Mai Lingling
|
Project Period (FY) |
2017-06-30 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2017: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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Keywords | Pax6 / 精子形成 / 性比バイアス / 脳科学 / 基盤・社会脳科学 / 脳計測科学 / 性比 / 遺伝子 / 発生・分化 / 老化 |
Outline of Final Research Achievements |
In this study, we examined whether the cause of biased sex ratio observed in spontaneous Pax6 mutant heterozygous (Sey/+) mice is in the process of spermatogenesis. Although the number of spermatogonia in the testis was similar between Sey/+ mice and wild-type littermates, the number of spermatocytes was significantly reduced in Sey/+ mice, suggesting that Pax6 dysfunction may affect the meiotic process. Furthermore, with fluorescent in situ hybridization for X and Y chromosomes, we also analyzed the proportion of sex chromosomes in round spermatids. Surprisingly, it became clear that the proportion of Y chromosome is significantly increased in round spermatids of Sey/+ mice. These results suggest that the biased sex ratio observed in Sey/+ mice may be due to the production of a large proportion of sperm having Y chromosome.
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Academic Significance and Societal Importance of the Research Achievements |
出生時の男女比は人種等に関わらずほぼ1.05対1であるが、その理由は不明であった。哺乳類の性は精子の性染色体によって決定され、減数分裂によりXおよびY染色体を持つ配偶子が同じ数だけ産生されるため、仔の性比は理論的には1:1となることが予想され、もし性バイアスが生じるのであれば、なんらかのメカニズムが必要となる。本研究は、減数分裂過程における転写制御因子・クロマチンリモデリング因子Pax6の機能不全が仔の性比に影響することを示した画期的な研究であり、今後その機構を明らかにすることで哺乳類の有する仔の性比調節において、精子形成の制御を介したエピジェネティックな機構が明らかになると期待される。
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Report
(3 results)
Research Products
(26 results)
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[Journal Article] SETDB1 is essential for mouse primordial germ cell fate determination by ensuring BMP signaling2018
Author(s)
Mochizuki Kentaro, Tando Yukio, Sekinaka Tamotsu, Otsuka Kei, Hayashi Yohei, Kobayashi Misato, Kamio Asuka, Ito-Matsuoka Yumi, Takehara Asuka, Kono Tomohiro, Osumi Noriko, Matsui Yasuhisa
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Journal Title
Development
Volume: 145
Issue: 23
Pages: 1-10
DOI
Related Report
Peer Reviewed
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[Journal Article] Repression of somatic genes by selective recruitment of HDAC3 by BLIMP1 is essential for mouse primordial germ cell fate determination2018
Author(s)
Mochizuki Kentaro, Hayashi Yohei, Sekinaka Tamotsu, Otsuka Kei, Ito-Matsuoka Yumi, Kobayashi Misato, Oki Shinya, Takehara Asuka, Kono Tomohiro, Osumi Noriko, Matsui Yasuhisa
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Journal Title
Cell Reports
Volume: 24
Issue: 10
Pages: 2682-2693
DOI
Related Report
Peer Reviewed / Open Access
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[Book] 脳の誕生2017
Author(s)
大隅 典子
Total Pages
272
Publisher
筑摩書房
ISBN
9784480071019
Related Report