Large-scale live cell imaging for elucidation of plant organogenesis
| Project/Area Number |
17K19380
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biology of Cells to Organisms, and related fields
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| Research Institution | Kumamoto University |
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Principal Investigator |
Higaki Takumi 熊本大学, 国際先端科学技術研究機構, 准教授 (90578486)
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| Project Period (FY) |
2017-06-30 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | ライブセルイメージング / 生物画像解析 / 細胞形態 / 細胞骨格 / 定量評価 / シロイヌナズナ / 形態形成 / 画像解析 / 植物 |
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| Outline of Final Research Achievements |
Despite the considerable progress in recent biology, we still have a large gap in our knowledge between cell and developmental biology. To elucidate how cell morphogenesis contributes to organogenesis, large-scale live cell imaging system is required. However, in order to realize such an advanced live cell imaging technique, there are problems such as laser phototoxicity and time consuming to process a big data of microscopic images. To overcome these problems, we aimed to optimize the monitoring conditions for plant cotyledon growth and develop the image analysis framework to quantitatively evaluate cell morphology and cytoskeletal organization based on large-scale microscopic images. With the support of these techniques, we also aimed to understand the mechanisms of plant cotyledon organogenesis at a single cell resolution.
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| Academic Significance and Societal Importance of the Research Achievements |
細胞と器官の形態形成の制御機構の解析はこれまで細胞生物学分野と発生生物学分野においてそれぞれ独立して進められてきた。しかし、これらの分子的な知見は必ずしも一致しておらず、生物の多階層的な形態形成制御機構を統一的に理解するには至っていない。本研究で開発した技術は、このような階層間の乖離を解消するための基盤要素技術と位置付けられる。本技術の成熟により、器官の発生や形態形成に対する細胞レベルでの現象の寄与が検証できるようになり、その結果として得られる知見は多階層的な生物形態形成機構の統一的な理解に資することが期待される。
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Report
(3 results)
Research Products
(23 results)
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[Journal Article] Regulation of Root-Knot Nematode Behavior by Seed-Coat Mucilage-Derived Attractants.2019
Author(s)
Tsai, A, Y-L., Higaki, T., Nguyen, C-N., Perfus-Barbeoch, L., Favery, B., and Sawa, S.
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Journal Title
Mol. Plants.
Volume: 12
Issue: 1
Pages: 99-122
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Differential Effects of Phosphatidylinositol 4-Kinase (PI4K) and 3-Kinase (PI3K) Inhibitors on Stomatal Responses to Environmental Signals.2017
Author(s)
Takahashi, S., Monda, K., Higaki, T., Negi, J., Hashimoto-Sugimoto, M., Hasezawa, S. and Iba, K.
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Journal Title
Front. Plant Sci.
Volume: 8
Pages: 677-677
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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