Tissue Pattern Formation Generated by Local Signal
| Project/Area Number |
17K19418
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biology of Cells to Organisms, and related fields
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| Research Institution | Center for Novel Science Initatives, National Institutes of Natural Sciences (2018-2019)
Okazaki Research Facilities, National Institutes of Natural Sciences (2017) |
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Principal Investigator |
Takada Shinji 大学共同利用機関法人自然科学研究機構(新分野創成センター、アストロバイオロジーセンター、生命創成探究, 生命創成探究センター, 教授 (60206753)
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| Project Period (FY) |
2017-06-30 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 遺伝子 / 細胞 / シグナル伝達 / 極性 / 発生・分化 / 細胞・組織 |
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| Outline of Final Research Achievements |
Secretory signal proteins, including Wnt, play roles in the formation of tissues and organs, but their spatial dynamics has yet been poorly understood. We found previously that secreted Wnt does not form a global concentration gradient but localizes on the cell membrane. In this study, by using the early development of Xenopus as a model system, we examined the relationship between Wnt11 and factors regulating the planar cell polarity (PCP factors). Our results suggest that Wnt is polarized by the interaction with PCP factors and that the polarized Wnt further induces the polarization of PCP factors. Such mutual induction appears to spread in a relay manner and polarizes the entire tissue.
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| Academic Significance and Societal Importance of the Research Achievements |
これまで、Wnt蛋白質は細胞間を自由拡散し組織の広い範囲に渡る「グローバルな濃度勾配」を形成すると考えられてきたが、本研究の結果はWntが極性を持って上皮シート上に分布することを示しており、これまで見過ごされてきたWnt蛋白質の「ローカルな局在化」の重要性を示唆している。さらに、このようなローカルな局在化は、Wntと PCP因子との相互作用によるものであり、それがさらにPCP因子の極性化を誘導することを本研究の結果は強く示唆している。このような相互誘導がリレー式に広がることにより組織全体に極性形成が起きるというモデルは、平面内細胞極性に形成機構に対して新たな考え方を提唱するものである。
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Report
(4 results)
Research Products
(36 results)
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[Journal Article] Assembly of protein complexes restricts diffusion of Wnt3a proteins2018
Author(s)
Takada, R., Mii, Y., Krayukhina, E., Maruyama, Y., Mio, K., Sasaki, Y., Shinkawa, T., Pack, C.-G., Sako, Y., Sato, C., Uchiyama, S., Takada, S.
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Journal Title
Commun. Biol.
Volume: 1
Issue: 1
Pages: 165-165
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Ripply3 is required for the maintenance of epithelial sheets in the morphogenesis of pharyngeal pouches2018
Author(s)
Tsuchiya, Y., Mii, Y., Okada, K., Furuse, M., Okubo, T., Takada, S.
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Journal Title
Dev. Growth Diff.
Volume: 60
Issue: 2
Pages: 87-96
DOI
Related Report
Peer Reviewed
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