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A database on in situ synaptic ultrastructures

Research Project

Project/Area Number 17K19446
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Research Field Neuroscience and related fields
Research InstitutionUniversity of Fukui

Principal Investigator

FUKAZAWA YUGO  福井大学, 学術研究院医学系部門, 教授 (60343745)

Co-Investigator(Kenkyū-buntansha) 石川 達也  金沢大学, 医学系, 助教 (00750209)
加藤 輝  大学共同利用機関法人自然科学研究機構(新分野創成センター、アストロバイオロジーセンター、生命創成探究, 生命創成探究センター, 特任助教 (30391915)
Project Period (FY) 2017-06-30 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2018: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywordsシナプス結合 / 微細構造 / 三次元再構築 / 電子顕微鏡 / 微細形態 / 定量解析 / FIB-SEM / 超微形態 / シナプス / 構造相関
Outline of Final Research Achievements

This study revealed quantitative ultrastructural information of 8 kinds of synaptic contacts at the nano-scale resolution and the statistically significant correlations between specific pairs of sub-synaptic ultrastructures, which in turn illustrated common and unique features in synaptic ultrastructures across these synaptic contacts. This study also demonstrated that neuronal activity and synaptic plasticity do not influence on the correlation between the sub-synaptic ultrastructures. Moreover, this study demonstrated novel roles of several genes in ultrastructural organization of the synapse.

Academic Significance and Societal Importance of the Research Achievements

本研究は脳内の複数のシナプス結合種の構造特徴を明らかにし、脳内シナプスの共通の構造則と固有則に関する知見を創出し、更には各種遺伝子のシナプス結合構造の構築への影響等を明らかにした。これらの知見は脳内神経回路の動作メカニズムや調節メカニズムを理解する基礎となり、更には神経疾患の病態解明の一助となることも期待できる。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (23 results)

All 2019 2018 2017 Other

All Int'l Joint Research (3 results) Journal Article (9 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 9 results,  Open Access: 9 results) Presentation (9 results) (of which Int'l Joint Research: 2 results,  Invited: 4 results) Remarks (2 results)

  • [Int'l Joint Research] Castilla-La Mancha University(Spain)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] IST Austria(Austria)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] University of Pittsburgh Medical School(米国)

    • Related Report
      2017 Research-status Report
  • [Journal Article] PIP3-Phldb2 is crucial for LTP regulating synaptic NMDA and AMPA receptor density and PSD95 turnover2019

    • Author(s)
      Xie Min-Jue、Ishikawa Yasuyuki、Yagi Hideshi、Iguchi Tokuichi、Oka Yuichiro、Kuroda Kazuki、Iwata Keiko、Kiyonari Hiroshi、Matsuda Shinji、Matsuzaki Hideo、Yuzaki Michisuke、Fukazawa Yugo、Sato Makoto
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 4305-4305

    • DOI

      10.1038/s41598-019-40838-6

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Microglial SIRPα regulates the emergence of CD11c+ microglia and demyelination damage in white matter.2019

    • Author(s)
      Sato-Hashimoto M, Nozu T, Toriba R, Horikoshi A, Akaike M, Kawamoto K, Hirose A, Hayashi H, Nagai H, Shimizu W, Saiki A., Ishikawa T, Elhanbaly R, Kotani T, Murata Y, Saito Y, Naruse M, Shibasaki K, Oldenborg P.-A, Jung S, Matozaki T, Fukazawa Y, Ohnishi H.
    • Journal Title

      eLife

      Volume: - Pages: 1-29

    • DOI

      10.7554/elife.42025

    • NAID

      120006604638

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Opposing roles of dopamine receptor D1- and D2-expressing neurons of the anteromedial olfactory tubercle in acquisition of place preference2019

    • Author(s)
      Koshi Murata, Tomoki Kinoshita, Yugo Fukazawa, Kenta Kobayashi, Akihiro Yamanaka, Takatoshi Hikida, Hiroyuki Manabe, and Masahiro Yamaguchi
    • Journal Title

      Frontiers in Behavioral Neuroscience

      Volume: 13 Pages: 50-50

    • DOI

      10.3389/fnbeh.2019.00050

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Layer-specific morphological and molecular differences in neocortical astrocytes and their dependence on neuronal layers2018

    • Author(s)
      Lanjakornsiripan Darin、Pior Baek-Jun、Kawaguchi Daichi、Furutachi Shohei、Tahara Tomoaki、Katsuyama Yu、Suzuki Yutaka、Fukazawa Yugo、Gotoh Yukiko
    • Journal Title

      Nature Communications

      Volume: 9 Issue: 1 Pages: 1623-1623

    • DOI

      10.1038/s41467-018-03940-3

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Elasticity-based boosting of neuroepithelial nukleokinesis via indirect energy transfer from mother to daughter2018

    • Author(s)
      Tomoyasu Shinoda, Arata Nagasaka, Yasuhiro Inoue, Ryo Higuchi, Yoshiaki Minami, Kagayaki Kato, Makoto Suzuki, Takefumi Kondo, Takumi Kawaue, Kanako Saito, Naoto Ueno, Yugo Fukazaawa, Masaharu Nagayama, Takashi Miura, Taiji Adachi, Takaki Miyata
    • Journal Title

      PLoS Biology

      Volume: 16 Issue: 4 Pages: 2004426-2004426

    • DOI

      10.1371/journal.pbio.2004426

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Optogenetic Control of Synaptic AMPA Receptor Endocytosis Reveals Roles of LTD in Motor Learning2018

    • Author(s)
      Kakegawa Wataru、Katoh Akira、Narumi Sakae、Miura Eriko、Motohashi Junko、Takahashi Akiyo、Kohda Kazuhisa、Fukazawa Yugo、Yuzaki Michisuke、Matsuda Shinji
    • Journal Title

      Neuron

      Volume: 99 Issue: 5 Pages: 985-998

    • DOI

      10.1016/j.neuron.2018.07.034

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] SK2 Channels Associate With mGlu1α Receptors and CaV2.1 Channels in Purkinje Cells2018

    • Author(s)
      Lujan Rafael、Aguado Carolina、Ciruela Francisco、Arus Xavier Morato、Martin-Belmonte Alejandro、Alfaro-Ruiz Rocio、Martinez-Gomez Jesus、de la Ossa Luis、Watanabe Masahiko、Adelman John P、Shigemoto Ryuichi、Fukazawa Yugo
    • Journal Title

      Frontiers in Cellular Neuroscience

      Volume: 12 Pages: 311-311

    • DOI

      10.3389/fncel.2018.00311

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Optogenetic Activation of Non-Nociceptive Aβ Fibers Induces Neuropathic Pain-LikeSensory and Emotional Behaviors after Nerve Injury in Rats.2018

    • Author(s)
      Tashima R, Koga K, Sekine M, Kanehisa K, Kohro Y, Tominaga K,Matsushita K, Tozaki-Saitoh H, Fukazawa Y, Inoue K, Yawo H, Furue H, Tsuda M
    • Journal Title

      eNeuro

      Volume: 5(1) Issue: 1 Pages: 0450-17

    • DOI

      10.1523/eneuro.0450-17.2018

    • NAID

      130007901054

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Differential association of GABAB receptors with their effector ion channels in Purkinje cells2017

    • Author(s)
      Lujan R, Aguado C, Ciruela F, Cozar J, Kleindienst D, de la Ossa L, Wickman K, Watanabe M, Shigemoto R, Fukazawa Y
    • Journal Title

      Brain Structure and Function

      Volume: 223 Issue: 3 Pages: 565-1587

    • DOI

      10.1007/s00429-017-1568-y

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 凍結割断レプリカ標識法と免疫タグノックインを組合せた神経細胞におけるNa+/K+ ATPase alpha 3の定量的膜上分布解析2019

    • Author(s)
      黒田一樹,石川達也,村田航志,深澤有吾
    • Organizer
      第124回日本解剖学会全国学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Construction rules of the axospinous synapses revealed by FIB-SEM imaging2019

    • Author(s)
      Yugo Fukazawa, Taito Sakurai, Ruwaida Elhanbaly, Tatsuya Ishikawa
    • Organizer
      9th Federation of the Asian and Oceanian Physiological Societies Congress
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 白質の損傷と修復を制御するミクログリアの分子シグナル2018

    • Author(s)
      永井裕美,橋本美穂,堀越絢乃,Ruwaida Elhanbaly,石川達也,的崎尚,深澤有吾,大西浩史
    • Organizer
      第61回日本神経化学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Construction rules of the axospinous synapse revealed by quantitative electron microscopy and novel structural anomalies of the synapse in gene-mutated mouse lines2018

    • Author(s)
      Yugo Fukazawa
    • Organizer
      第41回日本神経科学大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Endosomal Q-SNARE Syntaxin 7 specifies a subpopulation of recycling synaptic vesicles preferentially responsive to high frequency stimulation2018

    • Author(s)
      Yasunori Mori, Yugo Fukazawa, Shigeo Takamori
    • Organizer
      第70回日本細胞生物学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 軸索-棘シナプス結合の微細構造相関解析とその応用2018

    • Author(s)
      深澤有吾, Elhanbaly Ruwaida, 石川達也
    • Organizer
      日本解剖学会総会全国学術集会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] SDS-FRL法による定量的膜分子局在解析2017

    • Author(s)
      深澤有吾
    • Organizer
      日本細胞生物学会大会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Common and Unique Ultrastructural Rules across Axospinous Synapses in the Mouse Brain Revealed by FIB-SEM Imaging2017

    • Author(s)
      Yugo Fukazawa
    • Organizer
      Nepal-Japan Neurosymposium
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 凍結割断レプリカ免疫標識法を中心とした免疫電子顕微鏡法による分子局在解析の基礎と応用2017

    • Author(s)
      深澤有吾
    • Organizer
      新学術領域研究「先端モデル動物支援プラットフォーム」
    • Related Report
      2017 Research-status Report
    • Invited
  • [Remarks] 脳形態機能学(解剖学2)

    • URL

      https://www.med.u-fukui.ac.jp/laboratory/brain/

    • Related Report
      2018 Annual Research Report
  • [Remarks] 福井大学医学部脳形態機能学(解剖学2)

    • URL

      http://www-n.med.u-fukui.ac.jp/laboratory/brain/

    • Related Report
      2017 Research-status Report

URL: 

Published: 2017-07-21   Modified: 2022-02-21  

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