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Development of cancer suicide gene therapy using RNA trans-splicing technology

Research Project

Project/Area Number 17K19475
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Research Field Pharmaceutical Sciences and related fields
Research InstitutionChiba University

Principal Investigator

Furihata Tomomi  千葉大学, 大学院医学研究院, 講師 (80401008)

Co-Investigator(Kenkyū-buntansha) 秋田 英万  千葉大学, 大学院薬学研究院, 教授 (80344472)
Research Collaborator Kaneda Atsushi  
Project Period (FY) 2017-06-30 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywordsがん分子標的 / 核酸医薬 / ドラッグデリバリー / がん分子標的治療 / がん型OATP1B3 / がんドラッグデリバリーシステム / 転写リプログラミング
Outline of Final Research Achievements

The aim of this study is to develop a cancer gene therapy using RNA trans-splicing technology. We have constructed the therapeutic gene by combining the herpes simplex virus thymidine kinase gene with an RNA trans-splicing molecule targeting cancer-type organic anion transporting polypeptide 1B3. When introduced into human colon cancer cells, the therapeutic gene shows remarkable anti-cancer effects. We have also develop ssPalm-based lipid nanoparticles as a tool for delivery of the therapeutic gene into cancer cells. Collectively, our new cancer gene therapy with RNA trans-splicing technology and ssPalm-based gene delivery system has the potential to become a promising therapeutic option to fight against various cancer types.

Academic Significance and Societal Importance of the Research Achievements

最新科学を持ってしても未だ治療が困難ながん種は多く、がん死亡数も年々増加傾向にある。これに対し転写リプログラミング核酸医薬は、その効果ががん特異的に発揮されること等従来治療法とは異なる特徴を有し、さらに従来薬抵抗性の難治性がんに対する効果も期待される。したがって本研究成果は、転写リプログラミング核酸医薬開発の礎としてその更なる研究を促し、ひいては難治性がんの克服に貢献する治療法の開発に貢献するものと期待される。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (9 results)

All 2018 2017 Other

All Int'l Joint Research (2 results) Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results) Presentation (6 results) (of which Int'l Joint Research: 2 results)

  • [Int'l Joint Research] EB haus(オーストリア)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] EB haus(Austria)

    • Related Report
      2017 Research-status Report
  • [Journal Article] Cancer-type organic anion transporting polypeptide 1B3 is a target for cancer suicide gene therapy using RNA trans-splicing technology.2018

    • Author(s)
      Sun Y, Pinon Hofbauer J, Harada M, Woss K, Koller U, Morio H, Stierschneider A, Kitamura K, Hashimoto M, Chiba K, Akita H, Anzai N, Reichelt J, Bauer JW, Guttmann-Gruber C, Furihata T.
    • Journal Title

      Cancer Letters

      Volume: 433 Pages: 107-116

    • DOI

      10.1016/j.canlet.2018.06.032

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Cancer-type organic anion transporting polypeptide 1B3 is a promising target for spliceosome-mediated RNA trans-splicing based suicide gene therapy2018

    • Author(s)
      Hanae Morio, Yuchen Sun, Manami Harada, Ulrich Koller, Anna Stierschneider, Josefina Pinon Hofbauer, Christina Gruber, Hidetaka Akita, Naohiko Anzai, Kan Chiba, Tomomi Furihata.
    • Organizer
      18th World Congress of Basic and Clinical Pharmacology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Ct-SLCO1B3 as a biomarker for epidermolysis bullosa-associated skin cancer.2018

    • Author(s)
      Guttmann-Gruber C, Katharina Woss, Yuchen Sun Y, Furihata T, Reichelt J, Hofbauer JP.
    • Organizer
      International Investigative Dermatology 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] がん特異的遺伝子Cancer-type OATP1B3標的型RNA trans-splicing分子によるがん遺伝子治療の基盤構築2017

    • Author(s)
      森尾花恵、安西尚彦、降幡知巳
    • Organizer
      第76回日本癌学会学術総会
    • Related Report
      2017 Research-status Report
  • [Presentation] がん特異的遺伝子産物cancer-type OATP1B3を標的とした分子標的遺伝子治療法開発2017

    • Author(s)
      降幡知巳、孫雨晨、原田まなみ、Hofbauer P Josefina, Gruber Christina、秋田英万、安西尚彦
    • Organizer
      日本がん分子標的治療学会 第1回シーズ・ニーズ(SN)ワークショップ
    • Related Report
      2017 Research-status Report
  • [Presentation] がん特異的遺伝子Cancer-type OATP1B3標的型RNA trans-splicing分子によるがん遺伝子治療の基盤構築2017

    • Author(s)
      森尾花恵、降幡知巳、孫雨晨、原田まなみ、秋田英万、Ulrich Koller、Anna Stierschneider、Josefina Pion Hofbauer、Christina Gruber、千葉寛、安西尚彦
    • Organizer
      第136回日本薬理学会関東部会
    • Related Report
      2017 Research-status Report
  • [Presentation] がん特異的遺伝子Cancer-type OATP1B3を標的としたRNA trans-splicing分子によるがん自殺遺伝子治療の基盤確立2017

    • Author(s)
      井手秀行、孫雨晨、原田まなみ、森尾花恵、秋田英万、Ulrich Koller、Anna Stierschneider、Josefina Pion Hofbauer、Christina Gruber、千葉寛、安西尚彦、降幡知巳
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Research-status Report

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Published: 2017-07-21   Modified: 2020-03-30  

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