• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Elucidation of mechano-transfection and mechano-sensing mechanism using a living tissue

Research Project

Project/Area Number 17K19496
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Research Field Pharmaceutical Sciences and related fields
Research InstitutionNagasaki University

Principal Investigator

KAWAKAMI Shigeru  長崎大学, 医歯薬学総合研究科(薬学系), 教授 (20322307)

Co-Investigator(Kenkyū-buntansha) 麓 伸太郎  長崎大学, 医歯薬学総合研究科(薬学系), 准教授 (70380988)
Project Period (FY) 2017-06-30 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywordsメカノトランスフェクション / メカノセンサー分子 / 組織吸引圧法 / 組織押圧法 / 組織透明化 / トランスフェクション / transfection / gene therapy / gene delivery / mechanobiology / tissue cleaning
Outline of Final Research Achievements

In this study, we clarified the transgenic cells in each tissue of the mechano-transfection method, which is a gene transfer method by physical stimulation such as tissue pressure and suction, and analyzed the molecular organization molecule that responds to the physical stimulation. Regarding the mechano-transfection method, we succeeded in identifying gene-transfected cells in mouse liver, kidney, and heart, which were unknown until now. We also revealed a protein that fluctuates in the physical stimulation of mouse liver. On the other hand, when we newly developed an evaluation system for active oxygen involved in mechano-sensing in living tissue, no active oxygen was observed when physical stimulus was applied once, but active oxygen was detected when it was added 5 or 10 times.

Academic Significance and Societal Importance of the Research Achievements

本研究では、肝臓、腎臓、心臓といった生体組織への物理刺激による外来遺伝子導入時の遺伝子発現細胞を特定した。本研究成果は、生体組織への物理刺激を利用した遺伝子治療、遺伝子機能解析に応用できる。また、活性酸素の生体組織での組織透明化による新規評価系を構築した。さらに、生体組織への物理刺激の強さに応じて活性酸素が発生する可能性や物理刺激により変動するタンパク質の存在を明らかにすることができた。物理的刺激に応答するメカノセンシング機構は、単一ではなく、複数の機構が関与することが想定されることから、本研究成果により、メカノバイオロジーの研究対象が広がり、生命現象の包括的理解へと繋がることが期待される。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (19 results)

All 2020 2019 2018 2017 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 4 results) Presentation (11 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Effects of tissue pressure on transgene expression characteristics via renal local administration routes from ureter or renal artery in the rat kidney2020

    • Author(s)
      Natsuko Oyama, Haruyuki Takahashi, Maho Kawaguchi, Hirotaka Miyamoto, Koyo Nishida, Masako Tsurumaru, Mikiro Nakashima, Fumiyoshi Yamashita, Mitsuru Hashida, Shigeru Kawakami
    • Journal Title

      Pharmaceutics

      Volume: 12 Issue: 2 Pages: 114-114

    • DOI

      10.3390/pharmaceutics12020114

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Tissue suction-mediated gene transfer to the beating heart in mice2020

    • Author(s)
      Yota Taniguchi, Natsuko Oyama, Shintaro Fumoto, Hideyuki Kinoshita, Fumiyoshi Yamashita, Kazunori Shimizu, Mitsuru Hashida, Shigeru Kawakami
    • Journal Title

      PLoS ONE

      Volume: 15 Issue: 2 Pages: e0228203-e0228203

    • DOI

      10.1371/journal.pone.0228203

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 組織吸引デバイスによる生体組織変形をトリガーとする臓器への遺伝子導入技術2019

    • Author(s)
      清水一憲、川上 茂
    • Journal Title

      化学工業

      Volume: 83(7) Pages: 402-404

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Determining transgene expression characteristics using a suction device with multiple hole adjusting a left lateral lobe of the mouse liver2018

    • Author(s)
      Haraguchi A, Fuchigami Y, Kawaguchi M, Fumoto S, Ohyama K, Shimizu K, Hagimori M, Kawakami S
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 41

    • NAID

      130007382692

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Foreword2017

    • Author(s)
      Kawakami S
    • Journal Title

      Chemical and Pharmaceutical Bulletin

      Volume: 65 Issue: 7 Pages: 611-611

    • DOI

      10.1248/cpb.c17-ctf6507

    • NAID

      130006772513

    • ISSN
      0009-2363, 1347-5223
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 多色深部イメージングを利用した空間分布制御型ナノDDSの開発2019

    • Author(s)
      川上 茂、菅忠明
    • Organizer
      日本薬学会第139年会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 腎臓への圧力刺激を利用した遺伝子導入法:輸尿管投与・腎動脈投与後の腎臓内遺伝子発現と空間分布特性の解明2019

    • Author(s)
      大山奈津子、川口真帆、鶴丸雅子、萩森政頼、川上 茂
    • Organizer
      日本薬学会第139年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 肝臓吸引圧法におけるマウス肝臓内遺伝子発現特性の解明とメカノセンサー分子に関する評価2018

    • Author(s)
      原口綾奈、渕上由貴、大山 要、清水一憲、麓伸太郎、萩森政頼、川上 茂
    • Organizer
      第34回日本DDS学会学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] 腎臓における外来遺伝子発現・送達の多色深部イメージング2018

    • Author(s)
      大山奈津子、川口真帆、高比良慎、鶴丸雅子、萩森政頼、川上 茂
    • Organizer
      日本核酸医薬学会第4回年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Development of a multi-color deep imaging method for the evaluation of gene delivery system in the kidney2018

    • Author(s)
      N. Oyama, M. Kawaguchi, M. Takahira, M. Tsurumaru, M. Hagimori, S. Kawakami
    • Organizer
      18th Symposium for Gene, Design and Delivery
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 輸尿管投与による腎臓へのin vivo遺伝子導入における外来遺伝子発現分布の評価2018

    • Author(s)
      大山奈津子、川口真帆、高比良慎、鶴丸雅子、萩森政頼、川上 茂
    • Organizer
      遺伝子・デリバリー研究会 第18回夏期セミナー
    • Related Report
      2018 Research-status Report
  • [Presentation] 血管ならびに輸尿管経路による腎臓へのin vivo遺伝子導入における外来遺伝子発現分布の評価2018

    • Author(s)
      大山奈津子、川口真帆、高比良慎、鶴丸雅子、萩森政頼、橋田 充、川上 茂
    • Organizer
      第24回創剤フォーラム若手研究会
    • Related Report
      2018 Research-status Report
  • [Presentation] 医療イノベーションにおけるDDS2017

    • Author(s)
      川上 茂
    • Organizer
      第 41回西日本薬剤学研究会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 腎臓吸引圧法における吸引圧を施した腎臓での間質線維芽細胞特異的な遺伝子送達・発現2017

    • Author(s)
      大山奈津子、渕上由貴、麓伸太郎、萩森政頼、清水一憲、川上 茂
    • Organizer
      遺伝子・デリバリー研究会第17回シンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] 腎臓へのin vivo遺伝子導入における遺伝子発現・送達の空間分布2017

    • Author(s)
      大山奈津子、渕上由貴、川口真帆、萩森政頼、麓伸太郎、川上 茂
    • Organizer
      遺伝子・デリバリー研究会第17回夏季セミナー
    • Related Report
      2017 Research-status Report
  • [Presentation] Hepatic distribution characteristics of macromolecules in a suctioned liver using the tissue cleaning methods in mice2017

    • Author(s)
      Ayana Haraguchi, Yuki Fuchigami, Kaname Ohyama, Shintaro Fumoto, Masayori Hagimori, and Shigeru Kawakami
    • Organizer
      11th Young Investigator Symposium on Clinical Pharmaceutical Sciences
    • Related Report
      2017 Research-status Report
  • [Remarks] 長崎大学生命医科学域 医薬品情報学分野

    • URL

      http://www.ph.nagasaki-u.ac.jp/lab/arp/index-j.html

    • Related Report
      2019 Annual Research Report
  • [Remarks] 長崎大学 生命医科学域 医薬品情報学分野

    • URL

      http://www.ph.nagasaki-u.ac.jp/lab/arp/index-j.html

    • Related Report
      2018 Research-status Report 2017 Research-status Report
  • [Patent(Industrial Property Rights)] 生体由来材料の透明化用試薬2018

    • Inventor(s)
      麓伸太郎、川上 茂、西田孝洋
    • Industrial Property Rights Holder
      麓伸太郎、川上 茂、西田孝洋
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Related Report
      2017 Research-status Report
    • Overseas

URL: 

Published: 2017-07-21   Modified: 2021-02-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi