Search for novel Lgr5 ligands and receptor of Reg4
| Project/Area Number |
17K19584
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology and related fields
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2017-06-30 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | Lgr5 / Reg4 |
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| Outline of Final Research Achievements |
We have found that cooperation between Lgr5 and Reg4 is important for the tumorigenicity of colon cancer cells. It has been shown that LGR5 is an R-spondins receptor. However, we have obtained some preliminary data showing the existence of novel ligands for Lgr5. In this research project, we created a system that could purify Lgr5 and Reg4 binding proteins from cell culture medium and cell lysates by affinity chromatography.
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| Academic Significance and Societal Importance of the Research Achievements |
これまで、疾患発症におけるLgr5の役割や重要性は十分に明らかにされていなかった。そのため、Lgr5やその関連因子を標的とした治療法や診断法の開発は進んでいない。今後、本研究によって得られた知見を基にして新規Lgr5リガンドを同定することができれば、Lgr5関連シグナル経路の異常による癌発症メカニズムが明らかになるだけでなく、これまでに類を見ない分子標的治療法の開発にも繋がる可能性があると考える。
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Report
(3 results)
Research Products
(3 results)
