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Generation of the small human lungs as the novel ex vivo lung cancer study models

Research Project

Project/Area Number 17K19609
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Research Field Tumor biology and related fields
Research InstitutionNagasaki University

Principal Investigator

TSUCHIYA Tomoshi  長崎大学, 医歯薬学総合研究科(医学系), 准教授 (30437884)

Co-Investigator(Kenkyū-buntansha) 濱田 聖暁  長崎大学, 病院(医学系), 医員 (30796181)
永安 武  長崎大学, 医歯薬学総合研究科(医学系), 教授 (80284686)
Project Period (FY) 2017-06-30 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords肺再生 / 組織工学 / 臓器工学 / 脱細胞化 / がんモデル / 再生医療 / 脂肪由来間葉系細胞 / 疾患モデル / 肺がん / 薬剤効果 / スクリーニング / 研究モデル / 肺癌 / 再生医学 / 癌 / 移植・再生医療 / 肺
Outline of Final Research Achievements

We have fabricated small engineered lung using decellularized rat lung scaffold reseeded by rat whole lung cells, rat lung microvascular cells and rat adipogenic stromal cells. In the bioengineered lung, 4 types of human cancer cell lines (A549, PC-9, NCI-H520, PC-6) were seeded. Then, we have succeeded to establish an ex vivo lung cancer model, in which normal lung epithelial cells, capillary endothelial cells and cancer cells are living side by side.
In the ex vivo cancer model, cancer specific morphologies such as nodule or gland formations were observed, which are not formed in 2D culture. In addition, the drug responsiveness test in the ex vivo lung cancer model represented that gefitinib killed epidermal growth factor receptor (EGFR) mutation positive PC-9 cells with preserving surrounding normal lung cells.

Academic Significance and Societal Importance of the Research Achievements

本モデルは改良の途上であるが、より正確な薬剤スクリーニングや癌の生理学的研究に応用可能と考えられる。また、患者由来の細胞を使用すれば、薬剤の有効性など患者由来の再生臓器で評価でき、個別化医療に発展させる可能性を有している。さらに、癌研究にとどまらず、肺気腫モデルや肺線維化モデルなど他疾患の疾患研究モデルにもなりうる可能性を秘めている。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (8 results)

All 2020 2019 Other

All Presentation (2 results) Book (2 results) Remarks (1 results) Patent(Industrial Property Rights) (3 results) (of which Overseas: 1 results)

  • [Presentation] ラット再生肺を用いたヒト肺癌播種モデルの作製2020

    • Author(s)
      溝口聡
    • Organizer
      第22回日本異種移植研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 小型再生肺でのex vivo肺癌モデル作製の試み2019

    • Author(s)
      土谷智史
    • Organizer
      第46回日本臓器保存生物医学会学術集会
    • Related Report
      2019 Annual Research Report
  • [Book] 【再生医療の開発戦略と最新研究事例集】第12節 3次元構造を保った肺再生の取り組みと課題2019

    • Author(s)
      土谷智史
    • Total Pages
      8
    • Publisher
      技術情報協会
    • Related Report
      2019 Annual Research Report
  • [Book] 【バイオテクノロジーシリーズ;脱細胞化組織の作製法と医療・バイオ応用】肺における脱細胞化組織骨格を利用した臓器再生研究2019

    • Author(s)
      土谷智史
    • Total Pages
      8
    • Publisher
      シーエムシー出版
    • Related Report
      2019 Annual Research Report
  • [Remarks] Organ Engineering Research

    • URL

      https://www.organengineering.com/

    • Related Report
      2019 Annual Research Report
  • [Patent(Industrial Property Rights)] 疾患モデル2020

    • Inventor(s)
      土谷智史、永安武、溝口聡
    • Industrial Property Rights Holder
      土谷智史、永安武、溝口聡
    • Industrial Property Rights Type
      特許
    • Filing Date
      2020
    • Related Report
      2019 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 疾患モデル2019

    • Inventor(s)
      土谷智史、永安武、溝口聡
    • Industrial Property Rights Holder
      土谷智史、永安武、溝口聡
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-014778
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report
  • [Patent(Industrial Property Rights)] 疾患モデル2019

    • Inventor(s)
      土谷智史ほか
    • Industrial Property Rights Holder
      土谷智史ほか
    • Industrial Property Rights Type
      特許
    • Filing Date
      2019
    • Related Report
      2018 Research-status Report

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Published: 2017-07-21   Modified: 2023-01-30  

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