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Exploring image representation that enables to detect objects with little visible features and its application for protozoa identification

Research Project

Project/Area Number 17K20025
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Research Field Applied informatics and related fields
Research InstitutionJapan Advanced Institute of Science and Technology

Principal Investigator

Yoshitaka Atsuo  北陸先端科学技術大学院大学, 先端科学技術研究科, 准教授 (60263729)

Co-Investigator(Kenkyū-buntansha) 所 正治  金沢大学, 先進予防医学研究センター, 准教授 (30338024)
Research Collaborator Muhamad Kamal Mohammed Amin  
Pho Ngoc Dang Khoa  
Project Period (FY) 2017-06-30 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2018: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords寄生虫 / 原虫 / 物体検出 / 種の識別 / 局所特徴 / 分子疫学 / 分類 / 顕微鏡画像 / 画像特徴量 / スケール / 顕微鏡写真 / 大域・局所的特徴 / 空間周波数 / 画像とDNA情報 / 画像 / 情報検索 / 微生物 / 感染症
Outline of Final Research Achievements

Protozoa that is classified into parasite causing diseases can be identified not only by DNA analysis but also by its morphological features that is observed with microscope. In order to improve the accuracy of identifying species of parasite, we proposed to represent visible feature of a parasite with local features of different scale divisions. Detection of the region of parasite in micrograph is carried out by applying original deep neural network. Moreover, parasite database is enriched by epidemiological study for clarifying differences of related species.

Academic Significance and Societal Importance of the Research Achievements

寄生虫は顕微鏡のみで確定診断が可能な感染症の病原体である。寄生虫感染症は国内症例が極めて少なく、顕微鏡検査で寄生虫を検出した経験のある検査技師が少ないため、習熟度の低下が問題となっている。本研究では、種ごとに固有な視覚的特徴の差が大きくない原虫などの寄生虫をより精度高く識別する特徴量表現により虫体を識別する手法を提案し、また、寄生虫情報データベースの拡充により上記課題に対する情報提供システムの基盤を提供する意義がある。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (30 results)

All 2019 2018 2017 Other

All Int'l Joint Research (3 results) Journal Article (8 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 8 results,  Open Access: 1 results) Presentation (16 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results) Book (3 results)

  • [Int'l Joint Research] MJIIT(マレーシア)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Hasanuddin University(インドネシア)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] MJIIT(マレーシア)

    • Related Report
      2017 Research-status Report
  • [Journal Article] Possible pathogenicity of commensal Entamoeba hartmanni revealed by molecular screening of healthy school children in Indonesia2019

    • Author(s)
      Matsumura Takahiro、Hendarto Joko、Mizuno Tetsushi、Syafruddin Din、Yoshikawa Hisao、Matsubayashi Makoto、Nishimura Taro、Tokoro Masaharu
    • Journal Title

      Tropical Medicine and Health

      Volume: 47 Issue: 1 Pages: 1-7

    • DOI

      10.1186/s41182-018-0132-7

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Three monophyletic clusters in Retortamonas species isolated from vertebrates2019

    • Author(s)
      Hendarto J, Mizuno T, Hidayati APN, Rozi IE, Asih PBS, Syafruddin D, Yoshikawa H, Matsubayashi M, Tokoro M.
    • Journal Title

      Parasitol Int.

      Volume: 69 Pages: 93-98

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Protozoa Identification using 3D Geometric Multiple Color Channel Local Feature2018

    • Author(s)
      Khoa Pho, Takafumi Hirase, Muhamad Kamal Mohammed Amin, Atsuo Yoshitaka
    • Journal Title

      Proc. International Conference on Knowledge and System Engineering

      Volume: - Pages: 1-6

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Segmentation-driven RetinaNet for Protozoa Detection2018

    • Author(s)
      Khoa Pho, Muhamad Kamal Mohammed Aminy, Atsuo Yoshitaka
    • Journal Title

      Proc. IEEE International Conference on Multimedia

      Volume: - Pages: 1-8

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Molecular phylogeny of Blastocystis isolates from wild rodents captured in Indonesia and Japan2018

    • Author(s)
      Katsumata M, Yoshikawa H, Tokoro M, Mizuno T, Nagamoto T, Hendarto J, Asih PBS, Rozi IE, Kimata I, Takami K, Syafruddin D.
    • Journal Title

      Parasitol Res.

      Volume: 117 Pages: 2841-2846

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Extra-gastrointestinal anisakidosis caused by Pseudoterranova azarasi manifesting as strangulated inguinal hernia2017

    • Author(s)
      Mitsuboshi A, Yamaguchi H, Ito Y, Mizuno T, Tokoro M, Kasai M.
    • Journal Title

      Parasitol Int.

      Volume: 66 Issue: 6 Pages: 810-812

    • DOI

      10.1016/j.parint.2017.09.008

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] 分子生物学的手法によりPseudoterranova azarasiと同定された腸管外アニサキス症の一例2017

    • Author(s)
      水野 哲志、永元 健啓、所 正治、三星 アカリ、伊藤 雄介
    • Journal Title

      日本臨床寄生虫学会誌

      Volume: 28 Pages: 28-31

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] 胆嚢癌にともなう肝膿瘍から検出された口腔トリコモナス2017

    • Author(s)
      所 正治、松下萌未、西口恭平、谷口舞子、朝倉亜希子、森麻奈加、川崎洋輔、徳田貴昭、佐藤悠、齊木浩二、徳田有記、石見亜矢、大西良輝、河合知代、濱野美奈、千葉三保、山本克己、巽信之、日照田敦子、永元健啓
    • Journal Title

      日本臨床寄生虫学会誌

      Volume: 28 Pages: 90-92

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] 隠されたhaplotypeにより明らかになったGiardia intestinalis分子分類の新たな発見2019

    • Author(s)
      Tetsushi Mizuno, Hiroshi Ichimura, Din Syafruddin, Elijah Maritim Songok, Masaharu Tokoro
    • Organizer
      第88回日本寄生虫学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ブラストシスチス属の人獣共通感染性:インドネシア・スンバ島の10家族の住民とその家畜動物から分離された株について2019

    • Author(s)
      吉川尚男, 岩政綾菜, 所 正治, ディンシャフルディン
    • Organizer
      第88回日本寄生虫学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Protozoa Identification using 3D Geometric Multiple Color Channel Local Feature2018

    • Author(s)
      Khoa Pho, Takafumi Hirase, Muhamad Kamal Mohammed Amin, Atsuo Yoshitaka
    • Organizer
      International Conference on Knowledge and System Engineering
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Segmentation-driven RetinaNet for Protozoa Detection2018

    • Author(s)
      Khoa Pho, Muhamad Kamal Mohammed Aminy, Atsuo Yoshitaka
    • Organizer
      IEEE International Conference on Multimedia
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Detection and Identification of Parasite from Micrograph2018

    • Author(s)
      Atsuo Yoshitaka
    • Organizer
      India-Japan Bilateral Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 臨床寄生虫検査依頼の動向2018

    • Author(s)
      所 正治
    • Organizer
      第36回北陸病害動物研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ジアルジアの遺伝子型A/B特異的PCR法による分子スクリーニング2018

    • Author(s)
      瀬沼祥輝, 所正治
    • Organizer
      第36回北陸病害動物研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 腸管寄生アメーバ類の網羅的分子スクリーニング2018

    • Author(s)
      長田佳奈, 所正治
    • Organizer
      第36回北陸病害動物研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 胃癌切除標本中に認められたAnisakis pegreffiiの1例2018

    • Author(s)
      所正治, 原田憲一, 水野哲志, 前田泰之
    • Organizer
      第29回日本臨床寄生虫学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Are commensal protozoans truly non-pathogenic even in non-immune population?2018

    • Author(s)
      Masaharu Tokoro, Joko Hendarto, Din Syafruddin, Hisao Yoshikawa, Tetsushi Mizuno, Takehiro Nagamoto
    • Organizer
      the 14th International Congress of Parasitology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 寄生虫蔓延地域におけるジアルジアの病原性について2018

    • Author(s)
      所正治
    • Organizer
      第74回日本寄生虫学会西日本支部大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] First molecular surveillance of porcine Entamoeba suis and E. polecki in Central Java, Indonesia, April2018

    • Author(s)
      Hari Wardhana, Eny Martindah, Dyah Haryuningtyas Sawitri, Fitrine Ekawasti, Dias Aprita Dewi, Tomoyuki Shibahara, Masahiro Kusumoto, Masaharu Tokoro, Kazumi Sasai, Makoto Matsubayashi
    • Organizer
      第74回日本寄生虫学会西日本支部大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Giardia intestinalis assemblage Bのsub-genotypeと地域性について2018

    • Author(s)
      Tetsushi Mizuno, Elizabeth Matey, Joko Hendarto, Takehiro Nagamoto, Din Syafruddin, Hiroshi Ichimura, Elijah M Songok, Masaharu Tokoro
    • Organizer
      第74回日本寄生虫学会西日本支部大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 症例からみる寄生虫感染症2017

    • Author(s)
      所正治
    • Organizer
      第49回日本小児感染症学会総会・学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] 胆嚢癌にともなう肝膿瘍から検出された口腔トリコモナス2017

    • Author(s)
      所正治、松下萌未、西口恭平、谷口舞子、朝倉亜希子、森麻奈加、川崎洋輔、徳田貴昭、佐藤悠、齋木浩二、徳田有記、石見亜矢、大西良輝、河合知代、濱野美奈、千葉三保、山本克己、巽信之、日照田敦子、永元健啓
    • Organizer
      第28回日本臨床寄生虫学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 分子生物学的手法によりPseudoterranova decipensと同定された腸管外アニサキス症の一例2017

    • Author(s)
      水野哲志、永元健啓、三星アカリ、伊藤雄介、所正治
    • Organizer
      第28回日本臨床寄生虫学会大会
    • Related Report
      2017 Research-status Report
  • [Book] アメーバ赤痢.小児科臨床増刊・グローバル化・温暖化と感染症対策2017

    • Author(s)
      水野哲志、所 正治
    • Total Pages
      375
    • Publisher
      日本小児医事出版社
    • Related Report
      2017 Research-status Report
  • [Book] 寄生虫の遺伝子検査.メディカルテクノロジー2017

    • Author(s)
      水野 哲志、所 正治
    • Total Pages
      780
    • Publisher
      医歯薬出版
    • Related Report
      2017 Research-status Report
  • [Book] 赤痢アメーバ症、クリプトスポリジウム症.私の治療2017

    • Author(s)
      所 正治(分担)
    • Total Pages
      937
    • Publisher
      日本医事新報社
    • Related Report
      2017 Research-status Report

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Published: 2017-07-21   Modified: 2020-03-30  

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