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Preparation of gnotobiotic marine earthworm for pollutant decomposition and analysis of biological functions of endogenous bacteria

Research Project

Project/Area Number 17K20075
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Research Field Environmental conservation measure and related fields
Research InstitutionFisheries Research and Education Agency

Principal Investigator

Ito Katsutoshi  国立研究開発法人水産研究・教育機構, 瀬戸内海区水産研究所, 主任研究員 (80450782)

Co-Investigator(Kenkyū-buntansha) 太田 耕平  九州大学, 農学研究院, 准教授 (10585764)
伊藤 真奈  国立研究開発法人水産研究・教育機構, 瀬戸内海区水産研究所, 研究員 (60735900)
Project Period (FY) 2017-06-30 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2019: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords底質浄化 / 海産ミミズ / ノトバイオート / 底質汚染 / 内在菌 / 多環芳香族炭化水素 / バイオレメディエーション / ノトバイオートミミズ / 分解菌 / PAHs / 無菌化生物 / 化学物質分解菌 / 無菌海産ミミズ / 無菌化 / 無菌生物 / 環境浄化
Outline of Final Research Achievements

We aimed to develop sterile marine earthworms (Thalassodrilides cf. briani: Annelida: Clitellata: Naididae) and prepare gnotobiotic marine earthworms with pollutant-degrading bacteria. Two approaches were used to sterilize the eggs and parent earthworms. The eggs were successfully hatched on sterile medium and maintained aseptically for a certain period. Various antibiotics were used to sterilize parent earthworms, after 3 days of the tests, the number of bacterial cells decreased to detection limit levels, as determined using a quantitative PCR method. Sterile and gnotobiotic marine earthworms were prepared and subjected to pollutant decomposition and contamination resistance tests. The results demonstrated that the physiological functions such as resistance to pollutants differ greatly between the two populations of earthworms, proving that the endogenous bacteria play an important role in the process.

Academic Significance and Societal Importance of the Research Achievements

本研究成果から、海産ミミズの有する極めて高い汚染耐性・化学物質分解能に内在菌がどの様に関与しているのか、その一端が明らかになった。また、近年、Nature誌などに取り上げられている常在細菌叢に関する研究にも貢献するなど学術的に意義深い研究である。さらに、将来的には、汚染物質分解に特化したノトバイオート海産ミミズを用いた極めて効果的・革新的なバイオレメディエーション技術が社会に還元されることが十分にに期待されるなど、漁場保全学・ベントス学・微生物学の学術分野にも多大なインパクトがあるのみならず、底質のバイオレメディエーションに直結する、実学的要素を備えており、社会的な意義も大きいと考えられる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (12 results)

All 2019 2018 2017 Other

All Int'l Joint Research (2 results) Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results) Presentation (6 results) (of which Int'l Joint Research: 2 results) Funded Workshop (1 results)

  • [Int'l Joint Research] University of Silesia(ポーランド)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] University of Silesia(Poland)

    • Related Report
      2017 Research-status Report
  • [Journal Article] Marine sediment conservation using benthic organisms2019

    • Author(s)
      Katsutoshi Ito, Mana Ito, Takeshi Hano, Toshimitsu Onduka, Kazuhiko Mochida, Nobuaki Shono, Ryuhei Nakamura
    • Journal Title

      Bull. Jap. Fish. Res. Edu. Agen.

      Volume: 49 Pages: 45-51

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Seasonal variation of the biomass of the marine oligochaete <i>Thalassodrilides</i> cf. <i>briani</i> in Fukuura Bay, Japan and its optimal culture conditions2018

    • Author(s)
      伊藤 克敏, 伊藤 真奈, 太田 耕平, 羽野 健志, 大久保 信幸, 持田 和彦
    • Journal Title

      Japanese Journal of Benthology

      Volume: 73 Issue: 1 Pages: 57-63

    • DOI

      10.5179/benthos.73.57

    • NAID

      130007497182

    • ISSN
      1345-112X, 1883-891X
    • Year and Date
      2018-08-31
    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] The ovary organization in the marine limnodriloidin Thalassodrilides cf. briani (Annelida: Clitellata: Naididae) resembles the ovary of freshwater tubificins2018

    • Author(s)
      Anna Z. Urbisz, Lukasz Chajec, Mana Ito, Katsutoshi Ito
    • Journal Title

      Zoology

      Volume: 印刷中 Pages: 16-26

    • DOI

      10.1016/j.zool.2018.05.004

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] The ovary of Thalassodrilides cf. briani (Clitellata: Naididae: Limnodriloidinae) as the example of a multicellular germ-line cyst with a common cytoplasm2018

    • Author(s)
      Anna Z. Urbisz,Lukasz Chajec, Mana Ito, Katsutoshi Ito and Piotr Swiatek
    • Organizer
      14th International Symposium on Aquatic Oligochaeta
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Degradation of polycyclic aromatic hydrocarbons (PAHs) in marine sediment by interaction between oligochaete, Thalassodrilides cf. briani and microorganisms.2018

    • Author(s)
      Mana Ito, Katsutoshi Ito, Takeshi Hano, Motoharu Uchida
    • Organizer
      14th International Symposium on Aquatic Oligochaeta
    • Related Report
      2018 Research-status Report
  • [Presentation] Sterilization of marine oligochaete Thalassodrilides cf. briani (Clitellata: Naididae)2018

    • Author(s)
      Katsutoshi Ito, Mana Ito, Kohei Ohta
    • Organizer
      14th International Symposium on Aquatic Oligochaeta
    • Related Report
      2018 Research-status Report
  • [Presentation] 環境電位を指標にした底質浄化に関する研究2018

    • Author(s)
      伊藤 克敏・伊藤 真奈・持田 和彦・庄野 暢晃・中村 龍平
    • Organizer
      平成30年度日本水産学会春季大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Marine sediment conservation using benthic organisms2017

    • Author(s)
      Katsutoshi Ito, Mana Ito, Takeshi Hano, Toshimitsu Onduka, Kazuhiko Mochida, Ryuhei Nakamura, Nobuaki Shono
    • Organizer
      45th Scientific Symposium of the UJNR Aquaculture Panel
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 海産ミミズによる海洋底質の環境電位変動に伴う微生物群集構造の解析2017

    • Author(s)
      伊藤 真奈・梅澤 明夫・川市 智史・中村 龍平・伊藤 克敏
    • Organizer
      環境微生物系学会合同大会2017
    • Related Report
      2017 Research-status Report
  • [Funded Workshop] 14th International Symposium on Aquatic Oligochaeta2018

    • Related Report
      2018 Research-status Report

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Published: 2017-07-21   Modified: 2021-02-19  

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