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Development of photo-controlled morphogenesis for stem cell aggregates using photo-reactive matrices

Research Project

Project/Area Number 17K20105
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Research Field Biomedical engineering and related fields
Research InstitutionTohoku University

Principal Investigator

Yamamoto Masaya  東北大学, 工学研究科, 教授 (10332735)

Co-Investigator(Kenkyū-buntansha) 田畑 泰彦  京都大学, ウイルス・再生医科学研究所, 教授 (50211371)
Project Period (FY) 2017-06-30 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords立体組織 / 光加工 / 幹細胞凝集体 / オルガノイド / 光形態制御
Outline of Final Research Achievements

The technique of inducing the formation of a three-dimensional tissue from an aggregate of stem cells has attracted attention in regenerative medicine and drug discovery using organoids. In this study, we investigated a technique for modifying the spontaneous morphology control of cells by photo-controlled morphogenesis that combines hydrogel and photoreaction. As a result, it was clarified that the physical properties of hydrogel and the patterning of cell adhesion molecules influence the morphology of cells spontaneously forming. Furthermore, it was also shown that the new sandwich culture method using magnetic force facilitates three-dimensional culture.

Academic Significance and Societal Importance of the Research Achievements

幹細胞から体外で立体組織を構築する技術は、広範囲の学術的・社会的意義が期待される。すなわち、発生生物学の学術的発展に加え、再生医療で用いる移植組織や創薬に用いる動物実験代替物まで、様々な“ものづくり”に成り得る。このため、再生医療、創薬、材料科学など広い学術分野への波及効果が期待できる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (21 results)

All 2020 2019 2018 Other

All Int'l Joint Research (1 results) Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (16 results) (of which Int'l Joint Research: 8 results,  Invited: 11 results)

  • [Int'l Joint Research] Hanyang University(韓国)

    • Related Report
      2018 Research-status Report
  • [Journal Article] The Design of Sulfobetaine Polymers with Thermoresponsiveness under Physiological Salt Conditions2020

    • Author(s)
      Nobuyuki Morimoto, Yoshifumi Oishi, Masaya Yamamoto
    • Journal Title

      Macromolecular Chemistry and Physics

      Volume: 221 Issue: 5 Pages: 1900429-1900429

    • DOI

      10.1002/macp.201900429

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 3D in vitro Model of Vascular Medial Thickening in Pulmonary Arterial Hypertension2020

    • Author(s)
      Morii Chiharu、Tanaka Hiroyoshi Y.、Izushi Yasuhisa、Nakao Natsumi、Yamamoto Masaya、Matsubara Hiromi、Kano Mitsunobu R.、Ogawa Aiko
    • Journal Title

      Frontiers in Bioengineering and Biotechnology

      Volume: 8 Pages: 482-482

    • DOI

      10.3389/fbioe.2020.00482

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Preparation of cell aggregates incorporating gelatin hydrogel microspheres of sugar-responsive water-solubilization2020

    • Author(s)
      Inoo Kanako、Yamamoto Masaya、Tabata Yasuhiko
    • Journal Title

      Journal of Tissue Engineering and Regenerative Medicine

      Volume: 印刷中 Issue: 3 Pages: 210-219

    • DOI

      10.1002/term.307

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 未来を担う・未来に繋ぐ組織工学2018

    • Author(s)
      山本雅哉
    • Journal Title

      バイオマテリアル

      Volume: 36 Pages: 324-327

    • Related Report
      2018 Research-status Report
  • [Presentation] Engineering Cell Aggregates with Biomaterials2019

    • Author(s)
      Yamamoto Masaya
    • Organizer
      2019 BK21 Plus
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Smart Responsive Materials in Drug Delivery and Tissue Engineering2019

    • Author(s)
      Yamamoto Masaya
    • Organizer
      ASEAN + 5 Course on Bioceramics and Tissue Engineering
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 3D Tissue Processing Using Stimuli-Responsive Biomaterials2019

    • Author(s)
      Yamamoto Masaya
    • Organizer
      PRICM 10
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] In vitro tissue engineering by stimuli-responsive biomaterials2019

    • Author(s)
      Yamamoto Masaya
    • Organizer
      GFMAT/Bio-4
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 3次元培養のための酸化鉄ナノ粒子含有コラーゲンゲルの作製2019

    • Author(s)
      須藤泰河、山本雅哉
    • Organizer
      日本金属学会2019年秋期講演大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Magnetic Force - Assisted Sandwich Culture for Epithelial Cells2019

    • Author(s)
      Suto Taiga、Yamamoto Masaya
    • Organizer
      TERMIS-AP/ABMC2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 細胞膜上の生体分子と相互作用する生体材料の分子設計2019

    • Author(s)
      山本雅哉、最上譲二
    • Organizer
      高分子討論会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 再生医療2019

    • Author(s)
      山本雅哉
    • Organizer
      日本バイオマテリアル学会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 3次元管状上皮形成誘導のための磁性粒子含有ハイドロゲルの作製2019

    • Author(s)
      須藤泰河、山本雅哉
    • Organizer
      日本人工臓器学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Bioengineering Challenges Toward Regenerative Medicine2019

    • Author(s)
      Yamamoto Masaya
    • Organizer
      ICBD 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 生体機能性材料の再生医療への応用2019

    • Author(s)
      山本雅哉
    • Organizer
      第28回日本形成外科学会基礎学術集会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Magnetic Force-Assisted Sandwich Culture to Create 3D Microenvironment for Epithelial Cells2019

    • Author(s)
      Suto Taiga、Tokuda Mayumi、Yamamoto Masaya
    • Organizer
      MRM2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Organs-on-a-Chip開発における再生医療技術の重要性2019

    • Author(s)
      山本雅哉
    • Organizer
      第19回日本再生医療学会総会
    • Related Report
      2018 Research-status Report
  • [Presentation] Design of a Sacrificial Template to Fabricate Collagen Hydrogels with a Microchannel Structure2018

    • Author(s)
      山本雅哉
    • Organizer
      第35回国際フォトポリマーコンファレンス
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] ハイドロゲルを用いたin vitro組織形成2018

    • Author(s)
      山本雅哉
    • Organizer
      第1回日本金属学会第4分野講演会
    • Related Report
      2018 Research-status Report
  • [Presentation] 再生医療における組織工学の新展開2018

    • Author(s)
      山本雅哉
    • Organizer
      第55回再生医療ネットワーキング研究会
    • Related Report
      2018 Research-status Report
    • Invited

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Published: 2017-07-21   Modified: 2021-02-19  

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