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Structural basis of functional Hfq-binding region of bacterial sRNA

Research Project

Project/Area Number 17KK0146
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research)

Allocation TypeMulti-year Fund
Research Field Molecular biology
Research InstitutionKeio University (2020-2021)
Suzuka University of Medical Science (2017-2019)

Principal Investigator

MORITA Teppei  慶應義塾大学, 政策・メディア研究科(藤沢), 特任講師 (10444366)

Project Period (FY) 2017 – 2021
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
Keywords小分子RNA / 遺伝子発現 / 転写終結 / Hfq / 細菌 / 低温ショックタンパク質 / Rho / 機能性小分子RNA / 転写後制御 / 大腸菌
Outline of Final Research Achievements

This project focuses on structural features of the 3’end of bacterial sRNA and the mechanism of sRNA production via transcription termination. We investigated the termination efficiencies of some sRNAs and carried out a multicopy plasmid screen in E. coli to look for genetic factors by which the termination of sRNAs could be modulated. We identified novel factors that attenuate the termination of SgrS sRNA. Biochemical analyses showed effects of each factor on both the termination of sRNAs and the regulation by SgrS of the target mRNAs. These results confirm changes in the efficiency of intrinsic termination as an additional layer of the regulation of sRNA signaling. In addition, RNA-Seq analysis found global effects of CspD, which is one of the isolated factors, on transcription across some regulatory termination sites, such as Riboswithes. This suggests that CspD plays a global regulator in transcription elongation/termination.

Academic Significance and Societal Importance of the Research Achievements

近年のRNA-Seq技術の発展に伴い、細菌では、これまで考えられていたよりも頻繁に転写の伸長や終結がコントロールされており、細菌生理に関与する事例が報告されているが、転写伸長・終結の制御の分子機構は不明である。本研究において新規に同定した転写終結の抑制機能を持つ遺伝因子は、転写伸長・終結の制御機構の解明の一助となるものである。さらに、これらの因子を応用することで、病原性やバイオフィルムといった細菌生理の新たな制御技術の開発が期待できる。

Report

(6 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (11 results)

All 2022 2021 2020 2019 2018

All Int'l Joint Research (1 results) Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (9 results) (of which Int'l Joint Research: 3 results,  Invited: 3 results)

  • [Int'l Joint Research] National Institute of Health, National Cancer Institute(米国)2018

    • Year and Date
      2018-07-15
    • Related Report
      2021 Annual Research Report
  • [Journal Article] Regulation of Transcription Termination of Small RNAs and by Small RNAs: Molecular Mechanisms and Biological Functions2019

    • Author(s)
      Chen Jiandong、Morita Teppei、Gottesman Susan
    • Journal Title

      Frontiers in Cellular and Infection Microbiology

      Volume: 9 Pages: 1-9

    • DOI

      10.3389/fcimb.2019.00201

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Attenuators of transcription termination involved in sRNA signaling in Escherichia coli2022

    • Author(s)
      ○Teppei Morita, Nadim Majdalani, Masahiro C. Miura, Taku Oshima, Akio Kanai & Susan Gottesman
    • Organizer
      Microbes & RNA 2022 (6th meeting on Regulating with RNA in bacteria and Archaea)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Attenuator of transcriptional termination involved in production of small regulatory RNAs in Escherichia coli2021

    • Author(s)
      ○Teppei Morita, Nadim Majdalani, Masahiro C. Miura, Taku Oshima, Akio Kanai & Susan Gottesman
    • Organizer
      第22回 日本RNA学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 細菌小分子RNAの特性と制御機構2021

    • Author(s)
      ○森田鉄兵
    • Organizer
      第33回 微生物シンポジウム
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Isolation of factors affecting readthrough at an sRNA rho-independent terminator2021

    • Author(s)
      ○Teppei Morita, Nadim Majdalani, Masahiro C. Miura, Taku Oshima, Akio Kanai & Susan Gottesman
    • Organizer
      World Microbe Forum (ASM & FEMS)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] オミクス研究が明かす多階層での遺伝子発現の制御様式2020

    • Author(s)
      森田 鉄兵
    • Organizer
      第94回 日本感染症学会学術講演会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] The Role of transcription termination in sRNA biogenesis2020

    • Author(s)
      森田鉄兵
    • Organizer
      第93回 日本細菌学会総会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Screening for factors affecting read-through at an sRNA rho independent terminator2019

    • Author(s)
      森田 鉄兵、Nadim Majdalani, Susan Gottesman
    • Organizer
      第21回 RNA学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] Screening for factors affecting read-through at an sRNA rho independent terminator2019

    • Author(s)
      Teppei Morita, Nadim Majdalani, Susan Gottesman
    • Organizer
      the Gorden Research Conference on Mechanisms of Microbial transcription
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Screening for factors affecting read-through at sRNA rho-independent terminator2019

    • Author(s)
      Teppei Morita, Nadim Majdalani & Susan Gottesman
    • Organizer
      第13回ゲノム微生物学会年会(東京)
    • Related Report
      2018 Research-status Report

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Published: 2018-01-25   Modified: 2023-01-30  

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