Research Project
Fund for the Promotion of Joint International Research (Fostering Joint International Research)
Human hepatocyte chimeric mice were infected with hepatitis B virus and thier liver tissues were collected after sacrifice. Total RNA was extracted from the mouse livers and transcribed into cDNA. Then gene expression analyses were perfomred using these cDNA and gene expression profiles were compared between HBV-infected and non-infected mice. Resulting these analyses, we identified that similar viral kinetic pattern could be observed regardless to HBV clones and that pathways associated with immune responses, cell growth or apoptosis were significantly activated in human hepatocytes after HBV infection. Furthermore, we also identified that intracellular resposes by HBV infection were different among HBV genotypes.
年間50万人以上がB型肝炎ウイルス感染により死亡するとされ、特にアジア地域ではその感染者数が多い。そのため、B型慢性肝炎に対する治療薬の開発は急務であり、現在、治療薬開発に向けた研究が進められ、新規治療薬の登場が期待されている。このような背景から、治療薬開発において、抗ウイルス効果が評価可能な動物モデルの作製は非常に重要であり、また本研究のようなウイルス動態の視点から評価も必須と言える。本研究を進めていくことで、B型肝炎治療に向けた治療標的が同定され、本研究で得られた遺伝子発現プロファイルが新規治療薬開発の基盤として活用できる可能性がある。
All 2019 2018
All Int'l Joint Research (1 results) Journal Article (4 results) (of which Int'l Joint Research: 1 results, Peer Reviewed: 3 results, Open Access: 3 results) Presentation (7 results) (of which Int'l Joint Research: 4 results, Invited: 1 results)
Scientific Reports
Volume: 9 Issue: 1 Pages: 8462-8462
10.1038/s41598-019-44934-5
消化器・肝臓内科
Volume: 6 Pages: 271-278
Viruses
Volume: 11 Issue: 3 Pages: 263-263
10.3390/v11030263
Journal of Gastroenterology
Volume: 印刷中 Issue: 7 Pages: 650-659
10.1007/s00535-019-01558-w
