Understanding mechanisms of establishment and maintenance of coral holobiont

• Project/Area Number: 17KT0027
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Multi-year Fund
• Section: 特設分野
• Research Field: Constructive Systems Biology
• Research Institution: The University of Tokyo
• Principal Investigator: Shinzato Chuya 東京大学, 大気海洋研究所, 准教授 (70524726)
• Co-Investigator(Kenkyū-buntansha): 山下 洋 国立研究開発法人水産研究・教育機構, 水研機構(長崎), 主任研究員 (00583147) ; 將口 栄一 沖縄科学技術大学院大学, マリンゲノミックスユニット, 研究員 (90378563) ; 安岡 有理 国立研究開発法人理化学研究所, 生命医科学研究センター, 研究員 (70724954) ; 鈴木 豪 国立研究開発法人水産研究・教育機構, 水研機構(長崎), 主任研究員 (30533319) ; 座安 佑奈 沖縄科学技術大学院大学, マリンゲノミックスユニット, 研究員 (50746691)
• Project Period (FY): 2017-07-18 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000); Fiscal Year 2019: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2018: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2017: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: サンゴ / 褐虫藻 / 共生 / ゲノム / 遺伝子

Outline of Final Research Achievements

Corals are a symbiotic combination of reef-building corals and symbiotic algae (zooxanthellae) that survive as if they were a single organism, the holobiont. In this study, we utilized the genome information of corals and zooxanthellae to elucidate the principles of complicated coral "holobiont". By infecting coral larvae with cultured strains of symbiotic algae and conducting comprehensive gene expression analysis, we identified a group of coral genes involved in symbiosis. The functional estimates and evolutionary background of these genes were clarified, suggesting the diversity of symbiotic mechanisms in corals.

Academic Significance and Societal Importance of the Research Achievements

異なる褐虫藻感染株をサンゴ幼生へ感染させて、サンゴの遺伝子発現変動を網羅的に解析・比較することで、サンゴと褐虫藻の共生に重要な、サンゴの遺伝子群を特定することができた。これらのいくつかは、特定のサンゴ分類群で遺伝子重複により独自に誕生しており、サンゴの多様な共生メカニズムの存在が示唆された。さらにサンゴから単離された褐虫藻株、褐虫藻との共生様式が異なるサンゴの全ゲノム解読を行い、サンゴと褐虫藻の共生メカニズムをゲノム科学的に多方面から解析する研究基盤を構築した。

Research Products

• [Int'l Joint Research] 台湾海洋大学(その他の国・地域 台湾)
• [Journal Article] Correlation between Organelle Genetic Variation and RNA Editing in Dinoflagellates Associated with the Coral Acropora digitifera (2020)
  • Author(s): Shoguchi Eiichi、Yoshioka Yuki、Shinzato Chuya、Arimoto Asuka、Bhattacharya Debashish、Satoh Noriyuki
  • Journal Title: Genome Biology and Evolution Volume: 12 Issue: 3 Pages: 203-209
  • DOI: 10.1093/gbe/evaa042
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] De novo transcriptome assembly from the gonads of a scleractinian coral, Euphyllia ancora: molecular mechanisms underlying scleractinian gametogenesis (2020)
  • Author(s): Chiu Yi-Ling、Shikina Shinya、Yoshioka Yuki、Shinzato Chuya、Chang Ching-Fong
  • Journal Title: BMC Genomics Volume: 21 Issue: 1
  • DOI: 10.1186/s12864-020-07113-9
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Whole-Genome Transcriptome Analyses of Native Symbionts Reveal Host Coral Genomic Novelties for Establishing Coral?Algae Symbioses (2020)
  • Author(s): Yoshioka Yuki、Yamashita Hiroshi、Suzuki Go、Zayasu Yuna、Tada Ipputa、Kanda Miyuki、Satoh Noriyuki、Shoguchi Eiichi、Shinzato Chuya
  • Journal Title: Genome Biology and Evolution Volume: 13 Issue: 1 Pages: 1-18
  • DOI: 10.1093/gbe/evaa240
  • Peer Reviewed / Open Access
• [Journal Article] Eighteen Coral Genomes Reveal the Evolutionary Origin of Acropora Strategies to Accommodate Environmental Changes (2020)
  • Author(s): Shinzato Chuya、Khalturin Konstantin、Inoue Jun、Zayasu Yuna、Kanda Miyuki、Kawamitsu Mayumi、Yoshioka Yuki、Yamashita Hiroshi、Suzuki Go、Satoh Noriyuki
  • Journal Title: Molecular Biology and Evolution Volume: 38 Issue: 1 Pages: 16-30
  • DOI: 10.1093/molbev/msaa216
  • Peer Reviewed / Open Access
• [Journal Article] Discovery of a receptor guanylate cyclase expressed in the sperm flagella of stony corals (2019)
  • Author(s): Zhang Y, Chiu YL, Chen CJ, Ho YY, Shinzato C, Shikina S, Chang CF.
  • Journal Title: Scientific reports Volume: 9 Issue: 1 Pages: 1-9
  • DOI: 10.1038/s41598-019-51224-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Warm seawater temperature promotes substrate colonization by the blue coral, Heliopora coerulea (2019)
  • Author(s): Guzman C, Atrigenio M, Shinzato C, Alino P, Conaco C.
  • Journal Title: PeerJ Volume: e7785 Pages: e7785-e7785
  • DOI: 10.7717/peerj.7785
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A Siphonous Macroalgal Genome Suggests Convergent Functions of Homeobox Genes in Algae and Land Plants (2019)
  • Author(s): Arimoto A, Nishitsuji K, Higa Y, Arakaki N, Hisata K, Shinzato C, Satoh N, Shoguchi E.
  • Journal Title: DNA Research Volume: 26 Issue: 2 Pages: 183-192
  • DOI: 10.1093/dnares/dsz002
  • Peer Reviewed / Open Access
• [Journal Article] Medusozoan genomes inform the evolution of the jellyfish body plan (2019)
  • Author(s): Khalturin K, Shinzato C, Khalturina M, Hamada M, Fujie M, Koyanagi 5, Kanda M, Goto H, Anton-Erxleben F, Toyokawa M, Toshino S, Satoh N
  • Journal Title: Nature Ecology and Evolution Volume: 3(5) Issue: 5 Pages: 811-822
  • DOI: 10.1038/s41559-019-0853-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Symbiosis process between Acropora larvae and Symbiodinium differs even among closely related Symbiodinium types (2018)
  • Author(s): Yamashita H、Suzuki G、Shinzato C、Jimbo M、Koike K
  • Journal Title: Marine Ecology Progress Series Volume: 592 Pages: 119-128
  • DOI: 10.3354/meps12474
  • Peer Reviewed
• [Journal Article] Two divergent Symbiodinium genomes reveal conservation of a gene cluster for sunscreen biosynthesis and recently lost genes (2018)
  • Author(s): Shoguchi Eiichi、Beedessee Girish、Tada Ipputa、Hisata Kanako、Kawashima Takeshi、Takeuchi Takeshi、Arakaki Nana、Fujie Manabu、Koyanagi Ryo、Roy Michael C.、Kawachi Masanobu、Hidaka Michio、Satoh Noriyuki、Shinzato Chuya
  • Journal Title: BMC Genomics Volume: 19 Issue: 1 Pages: 458-458
  • DOI: 10.1186/s12864-018-4857-9
  • Peer Reviewed / Open Access
• [Journal Article] Transcriptome analysis of the reef-building octocoral, Heliopora coerulea (2018)
  • Author(s): Guzman Christine、Shinzato Chuya、Lu Tsai-Ming、Conaco Cecilia
  • Journal Title: Scientific Reports Volume: 8 Issue: 1
  • DOI: 10.1038/s41598-018-26718-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Metabolic co-dependence drives the evolutionarily ancient Hydra?Chlorella symbiosis (2018)
  • Author(s): Hamada Mayuko、Schr?der Katja、Bathia Jay、K?rn Ulrich、Fraune Sebastian、Khalturina Mariia、Khalturin Konstantin、Shinzato Chuya、Satoh Nori、Bosch Thomas CG
  • Journal Title: eLife Volume: 7
  • DOI: 10.7554/elife.35122
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Using Seawater to Document Coral-Zoothanthella Diversity: A New Approach to Coral Reef Monitoring Using Environmental DNA (2018)
  • Author(s): Shinzato C, Zayasu Y, Kanda M, Kawamitsu M, Satoh N, Yamashita H and Suzuki G
  • Journal Title: Frontiers in Marine Science Volume: 5 Pages: 28-28
  • DOI: 10.3389/fmars.2018.00028
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Using genomics to reveal novel insights into coral biology (2021)
  • Author(s): Chuya Shinzato
  • Organizer: Genome Concept Centennial Conference
  • Int'l Joint Research / Invited
• [Presentation] サツマハオリムシの全ゲノム解読と遺伝子予測 (2021)
  • Author(s): 吉田悠、善岡祐輝、八巻鮎太、佐々木章、井上広滋、新里宙也
  • Organizer: 令和3年度日本水産学会春季大会
• [Presentation] 18種のミドリイシ科サンゴの全ゲノム解読が明らかにする，ミドリイシ属の環境適応戦略 (2020)
  • Author(s): 新里宙也、Konstantin Khalturin 、井上潤、座安佑奈、神田美幸、川満真由美、善岡祐輝、山下洋、鈴木豪、佐藤矩行
  • Organizer: 日本サンゴ礁学会第23回大会
• [Presentation] 造礁サンゴ、コユビミドリイシのゲノムワイドSNPを用いた、南西諸島における集団構造解析 (2020)
  • Author(s): 土屋考人、座安佑奈、中島祐一、鈴木豪、佐藤矩行、新里宙也
  • Organizer: 日本サンゴ礁学会第23回大会
• [Presentation] 高精度な比較ゲノムを可能とするミドリイシ科サンゴからの遺伝子モデル構築 (2020)
  • Author(s): 善岡祐輝、山下洋、鈴木豪、新里宙也
  • Organizer: 日本サンゴ礁学会第23回大会
• [Presentation] サンゴ特異的 Notch 様遺伝子が、褐虫藻との共生関係を制御している ? (2019)
  • Author(s): 善岡祐輝、山下洋、邱顗陵、蔡品萱、座安佑奈、鈴木豪、識名信也、新里宙也
  • Organizer: 日本サンゴ礁学会第22回大会
• [Presentation] Durusdinium属褐虫藻とAcropora tenuis 幼生の初期共生 (2019)
  • Author(s): 山下 洋，鈴木 豪，新里 宙也，善岡 祐輝
  • Organizer: 日本サンゴ礁学会第22回大会
• [Presentation] Application of genomics to coral biology (2018)
  • Author(s): Chuya Shinzato
  • Organizer: 6th Taiwan-Japan Ecology Workshop
  • Int'l Joint Research / Invited
• [Presentation] Conservation and restoration of coral reefs: the most diverse marine ecosystems (2018)
  • Author(s): Chuya Shinzato
  • Organizer: 5th Asian conference on Safety and Education in Laboratory
  • Int'l Joint Research / Invited
• [Presentation] Coral genome project for understanding the most diverse of all marine ecosystems (2018)
  • Author(s): Chuya Shinzato
  • Organizer: 1st Korea-Japan (OIST) Joint International Symposium on Marine Genomics
  • Int'l Joint Research / Invited
• [Presentation] Genomics approaches for investigating probable cross-talk between coral and algae (2018)
  • Author(s): Chuya Shinzato
  • Organizer: ARC CoE Boden Research Conference “Origins and Function of Animal Metaorganism”, Townsville, Australia
  • Int'l Joint Research / Invited
• [Presentation] ゲノム科学的手法による造礁サンゴの遺伝的多様性の理解 (2017)
  • Author(s): 新里宙也
  • Organizer: 日本動物学会 第36回ホヤの生物学談話会
  • Invited
• [Presentation] ゲノム科学的手法によるサンゴ共生系の理解 (2017)
  • Author(s): 新里宙也
  • Organizer: 第1回日本共生生物学会大会
  • Invited