Establishment of the detection method for the reactivation of toxoplasmosis and the evaluatiion method for the severity of toxoplasmosis by mass spectrometer

• Project/Area Number: 17KT0124
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 特設分野
• Research Field: Complex Systems Disease Theory
• Research Institution: Chiba University
• Principal Investigator: NOROSE Kazumi 千葉大学, 大学院医学研究院, 特任准教授 (30156244)
• Co-Investigator(Kenkyū-buntansha): 彦坂 健児 千葉大学, 大学院医学研究院, 講師 (30456933) ; 佐藤 守 千葉大学, 医学部附属病院, 特任准教授 (20401002) ; 野村 文夫 千葉大学, 医学部附属病院, 特任教授 (80164739)
• Project Period (FY): 2017-07-18 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: トキソプラズマ症 / 質量分析 / 再活性化 / 重症化 / トキソプラズマ / ステージ変換 / 再活性化検出法 / 重症度評価法 / シスト

Outline of Final Research Achievements

Toxoplasmosis is caused by the infection with the protozoan parasite, Toxoplasma gondii.As an important event in the pathogenesis of toxoplasmosis is the interconversion between the bradyzoite and the tachyzoite stage of T. gondii within the intermediate host, to analyze the factors that influence either cyst formation or reactivation is essential.
In this report, we analyzed the stage conversion in vitro by changing the condition of medium and the culture supernatant was stored for protein level analysis by mass spectrometry. For in vivo analysis, mice were infected and serum were stored at the acute and chronic stages for the further analysis by mass spectrometry. In addition, residual samples from hospitals for diagnosis of toxoplasmosis were classified according to the severity of the disease for the analysis by mass spectrometry.

Academic Significance and Societal Importance of the Research Achievements

トキソプラズマ症は細胞内寄生虫のトキソプラズマによる感染症であり、世界中では人口の３分の一が感染しているとされる日和見感染症である。免疫状態の正常な人は無症状であるが、免疫不全状態の人は、トキソプラズマ症が再活性化したり、重症化し致死となることが問題となっている。トキソプラズマは宿主の免疫状態により、病原性の異なるステージ変換を行うが、それに関与する因子を質量分析計によりタンパク質レベルで解析することで、病原性や免疫不全状態での再発機序を解明でき、予後を左右する早期診断・早期治療につながると期待される。

Research Products

• [Journal Article] Hypericum erectum alcoholic extract inhibits Toxoplasma growth and Entamoeba encystation: an exploratory study on the anti-protozoan potential. (2020)
  • Author(s): Shinjyo N, Nakayama H, Ishimaru K, Hikosaka K, Mi-Ichi F, Norose K, Yoshida H.
  • Journal Title: J Nat Med Volume: 74 Issue: 1 Pages: 294-305
  • DOI: 10.1007/s11418-019-01369-6
  • NAID: 40022130373
  • Peer Reviewed
• [Journal Article] Desensitization to trimethoprim-sulfamethoxazole in a toxoplasmic encephalitis patient who was intolerant to conventional treatments. (2020)
  • Author(s): Miyata N, Yoshimura Y, Hikosaka K, Norose K, Tachikawa N.
  • Journal Title: J Infect Chemother Volume: 26 Issue: 3 Pages: 289-293
  • DOI: 10.1016/j.jiac.2019.10.008
  • Peer Reviewed
• [Journal Article] Genetic polymorphisms in malaria vaccine candidate Plasmodium falciparum reticulocyte-binding protein homologue-5 among populations in Lagos, Nigeria. (2020)
  • Author(s): Ajibaye O, Osuntoki AA, Balogun EO, Olukosi YA, Iwalokun BA, Oyebola KM, Hikosaka K, Watanabe YI, Ebiloma GU, Kita K, Amambua-Ngwa A.
  • Journal Title: Malar J. Volume: 19 Issue: 1 Pages: 6-6
  • DOI: 10.1186/s12936-019-3096-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An in-house centrifugation and membrane filtration technique for identifying microorganisms from positive blood culture bottles with high identification rates using matrix-assisted laser desorption ionization?Time-of-flight mass spectrometry: A preliminary report (2020)
  • Author(s): Tsuchida Sachio、Murata Syota、Miyabe Akiko、Satoh Mamoru、Takiwaki Masaki、Matsushita Kazuyuki、Nomura Fumio
  • Journal Title: Journal of Infection and Chemotherapy Volume: 26 Issue: 3 Pages: 266-271
  • DOI: 10.1016/j.jiac.2019.09.017
  • Peer Reviewed / Open Access
• [Journal Article] Post-transcriptional regulation of BRG1 by FIRΔexon2 in gastric cancer (2020)
  • Author(s): Ailiken Guzhanuer、Kitamura Kouichi、Hoshino Tyuji、Satoh Mamoru、Tanaka Nobuko、Minamoto Toshinari、Rahmutulla Bahityar、Kobayashi Sohei、Kano Masayuki、Tanaka Tomoaki、Kaneda Atsushi、Nomura Fumio、Matsubara Hisahiro、Matsushita Kazuyuki
  • Journal Title: Oncogenesis Volume: 9 Issue: 2 Pages: 26-26
  • DOI: 10.1038/s41389-020-0205-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Metastatic Malignant Lymphoma Mimicking Cerebral Toxoplasmosis with the "Target Sign" (2019)
  • Author(s): Ueno H, Norose K, Kamimura T, Mihara K, Yamasaki F, Hikosaka K, J Amatya V, Takeshima Y, Kurisu K, Maruyama H
  • Journal Title: Internal Medicine Volume: 58 Issue: 8 Pages: 1157-1162
  • DOI: 10.2169/internalmedicine.1156-18
  • NAID: 130007632763
  • ISSN: 0918-2918, 1349-7235
  • Year and Date: 2019-04-15
  • Peer Reviewed / Open Access
• [Journal Article] Toxoplasmosis-associated central nervous system vasculitis accompanied by multiple cerebral hemorrhages developing subsequent to cord blood transplantation (2019)
  • Author(s): 太田 貴徳, 今永 博, 奥 誠道, 楠元 大岳, 杉尾 康浩, 田宮 貞史, 久保 安孝, 小川 亮介, 彦坂 健児, 野呂瀬 一美, 大野 裕樹
  • Journal Title: Rinsho Ketsueki Volume: 60 Issue: 2 Pages: 118-123
  • DOI: 10.11406/rinketsu.60.118
  • NAID: 130007607569
  • ISSN: 0485-1439, 1882-0824
  • Peer Reviewed
• [Journal Article] Anti-FIRΔexon2, a splicing variant form of PUF60, auto-antibody is detected in the sera of esophageal squamous cell carcinoma. (2019)
  • Author(s): Kobayashi S, Hiwasa T, Ishige T, Rahmutulla B, Kano M, Hoshino T, Minamoto T, Shimada H, Nomura F, Matsubara H, Matsushita K.
  • Journal Title: Cancer Sci. Volume: 4 Issue: 6 Pages: 1-10
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• [Journal Article] Identification of conjugation positions of urinary glucuronidated vitamin D 3 metabolites by LC/ESI‐MS/MS after conversion to MS/MS‐fragmentable derivatives (2019)
  • Author(s): Yoshimura Yusuke、Hibi Ryuichiro、Nakata Akiho、Togashi Moeka、Ogawa Shoujiro、Ishige Takayuki、Satoh Mamoru、Nomura Fumio、Higashi Tatsuya
  • Journal Title: Biomedical Chromatography Volume: - Issue: 7
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  • Peer Reviewed / Open Access
• [Journal Article] Evaluation of analytical factors associated with targeted MEFV gene sequencing using long-range PCR/massively parallel sequencing of whole blood DNA for molecular diagnosis of Familial Mediterranean fever (2019)
  • Author(s): Ishige Takayuki、Itoga Sakae、Kawasaki Kenji、Utsuno Emi、Beppu Minako、Sawai Setsu、Nishimura Motoi、Ichikawa Tomohiko、Nomura Fumio、Matsushita Kazuyuki
  • Journal Title: Clinica Chimica Acta Volume: 495 Pages: 562-569
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• [Journal Article] Attitudes of clinical geneticists and certified genetic counselors to genome editing and its clinical applications: A nation-wide questionnaire survey in Japan (2019)
  • Author(s): Taguchi Iku、Yamada Takahiro、Akaishi Rina、Imoto Issei、Kurosawa Kenji、Nakatani Kaname、Nomura Fumio、Hamanoue Haruka、Hyodo Maki、Murakami Hiromi、Yoshihashi Hiroshi、Yotsumoto Junko、Kosugi Shinji
  • Journal Title: Journal of Human Genetics Volume: 64 Issue: 9 Pages: 945-954
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  • Peer Reviewed
• [Journal Article] A simple method for sequencing the whole human mitochondrial genome directly from samples and its application to genetic testing (2019)
  • Author(s): Yao Yue、Nishimura Motoi、Murayama Kei、Kuranobu Naomi、Tojo Satomi、Beppu Minako、Ishige Takayuki、Itoga Sakae、Tsuchida Sachio、Mori Masato、Takayanagi Masaki、Yokoyama Masataka、Yamagata Kazuyuki、Kishita Yoshihito、Okazaki Yasushi、Nomura Fumio、Matsushita Kazuyuki、Tanaka Tomoaki
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 17411-17421
  • DOI: 10.1038/s41598-019-53449-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Multiplex PCR and multicolor probes melting for the simultaneous detection of five UGT1A1 variants (2019)
  • Author(s): Ishige Takayuki、Itoga Sakae、Kawasaki Kenji、Sawai Setsu、Nishimura Motoi、Nomura Fumio、Matsushita Kazuyuki
  • Journal Title: Analytical Biochemistry Volume: 587 Pages: 113448-113448
  • DOI: 10.1016/j.ab.2019.113448
  • Peer Reviewed / Open Access
• [Journal Article] A novel caged Cookson‐type reagent toward a practical vitamin D derivatization method for mass spectrometric analyses (2019)
  • Author(s): Seki Masahiko、Sato Makoto、Takiwaki Masaki、Takahashi Koji、Kikutani Yoshikuni、Satoh Mamoru、Nomura Fumio、Kuroda Yutaka、Fukuzawa Seketsu
  • Journal Title: Rapid Communications in Mass Spectrometry Volume: 34 Issue: 7
  • DOI: 10.1002/rcm.8648
  • Peer Reviewed / Open Access
• [Journal Article] 原発性脳リンパ腫を合併したトキソプラズマ脳炎の再発例 (2019)
  • Author(s): 鷲野 巧弥, 矢嶋 敬史郎, 福島 一彰, 佐々木 秀悟, 関谷 綾子, 関谷 紀貴, 柳澤 如樹, 野呂瀬 一美, 味澤 篤, 今村 顕史.
  • Journal Title: 感染症学雑誌 Volume: -
  • NAID: 130007754738
  • Peer Reviewed
• [Journal Article] Infection dynamics of Toxoplasma gondii in gut-associated tissues after oral infection: The role of Peyer's patches. (2019)
  • Author(s): Mitsunaga T, Norose K, Aosai F, Horie H, Ohnuma N, Yano A
  • Journal Title: Parasitol Int Volume: 68 Issue: 1 Pages: 40-47
  • DOI: 10.1016/j.parint.2018.08.010
  • Peer Reviewed
• [Journal Article] Disseminated toxoplasmosis with atypical symptoms which developed with exacerbation of systemic lupus erythematosus. (2019)
  • Author(s): Furuya H, Ikeda K, Iida K, Suzuki K, Furuta S, Tamachi T, Suzuki K, Miura G, Hiraguri M, Hase R, Hikosaka K, Norose K, Nakajima H
  • Journal Title: Lupus Volume: 28 Issue: 1 Pages: 133-136
  • DOI: 10.1177/0961203318815583
  • Peer Reviewed
• [Journal Article] Cerebral Toxoplasmosis Diagnosed by Nested-polymerase Chain Reaction in a Patient with Rheumatoid Arthritis (2018)
  • Author(s): Matsuura J, Fujii A, Mizuta I, Norose K, Mizuno T.
  • Journal Title: Internal Medicine Volume: 57 Issue: 10 Pages: 1463-1468
  • DOI: 10.2169/internalmedicine.0139-17
  • NAID: 130006734390
  • ISSN: 0918-2918, 1349-7235
  • Year and Date: 2018-05-15
  • Peer Reviewed / Open Access
• [Journal Article] The complete mitochondrial genome of Sarcoptes scabiei var. nyctereutis from the Japanese raccoon dog: Prediction and detection of two transfer RNAs (tRNA-A and tRNA-Y) (2018)
  • Author(s): Ueda T, Tarui H, Kido N, Imaizumi K, Hikosaka K, Abe T, Minegishi D, Tada Y, Nakagawa M, Tanaka S, Omiya T, Morikaku K, Kawahara M, Kikuchi-Ueda T, Akuta T, Ono Y
  • Journal Title: Genomics Volume: - Issue: 6 Pages: 1183-1191
  • DOI: 10.1016/j.ygeno.2018.09.002
  • Peer Reviewed
• [Journal Article] Identification of specific and common diagnostic antibody markers for gastrointestinal cancers by SEREX screening using testis cDNA phage library (2018)
  • Author(s): Kobayashi Sohei、Hiwasa Takaki、Arasawa Takahiro、Kagaya Akiko、Ishii Sayaka、Shimada Hideaki、Ito Masaaki、Suzuki Masae、Kano Masayuki、Rahmutulla Bahityar、Kitamura Kouichi、Sawabe Yuji、Shin Hideo、Takiguchi Masaki、Nomura Fumio、Matsubara Hisahiro、Matsushita Kazuyuki
  • Journal Title: Oncotarget Volume: 9 Issue: 26 Pages: 18559-18569
  • DOI: 10.18632/oncotarget.24963
  • Peer Reviewed / Open Access
• [Journal Article] Disturbed alternative splicing of FIR (PUF60) directed cyclin E overexpression in esophageal cancers (2018)
  • Author(s): Ogura Yukiko、Hoshino Tyuji、Tanaka Nobuko、Ailiken Guzhanuer、Kobayashi Sohei、Kitamura Kouichi、Rahmutulla Bahityar、Kano Masayuki、Murakami Kentarou、Akutsu Yasunori、Nomura Fumio、Itoga Sakae、Matsubara Hisahiro、Matsushita Kazuyuki
  • Journal Title: Oncotarget Volume: 9 Issue: 33 Pages: 22929-22944
  • DOI: 10.18632/oncotarget.25149
  • Peer Reviewed / Open Access
• [Journal Article] Determination of serum carbohydrate-deficient transferrin by a nephelometric immunoassay for differential diagnosis of alcoholic and non-alcoholic liver diseases (2018)
  • Author(s): Nomura F, Kanda T, Seimiya M, Satoh M, Kageyama Y, Yamashita T, Yokosuka O, Kato N, Maruyama K.
  • Journal Title: Clin Chim Acta Volume: 485 Pages: 181-186
  • DOI: 10.1016/j.cca.2018.06.040
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• [Journal Article] Search for a Novel Allergen in Hen's Egg Allergy Using an IgE Immunoblotting Assay. (2018)
  • Author(s): Sogawa K, Takahashi Y, Shibata Y, Satoh M, Kodera Y, Nomura F, Tanaka T, Sato H, Yamaide F, Nakano T, Iwahashi K, Sugita-Konishi Y, Shimada A, Shimojo N.
  • Journal Title: Int Arch Allergy Immunol Volume: 176 Issue: 3-4 Pages: 189-197
  • DOI: 10.1159/000488144
  • Peer Reviewed
• [Journal Article] Capsular serotyping of Haemophilus influenzae by using matrix-associated laser desorption ionization-time of flight mass?spectrometry. (2018)
  • Author(s): Takeuchi N, Segawa S, Ishiwada N, Ohkusu M, Tsuchida S, Satoh M, Matsushita K, Nomura F
  • Journal Title: Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy Volume: 18 Issue: 7 Pages: 300062-66
  • DOI: 10.1016/j.jiac.2018.02.007
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Application of the biocopolymer preparation system, rapid BACproII kit, for mass-spectrometry-based bacterial identification from positive blood culture bottles by the MALDI Biotyper system. (2018)
  • Author(s): Tsuchida S, Murata S, Miyabe A, Satoh M, Takiwaki M, Ashizawa K, Terada T, Ito D, Matsushita K, Nomura F.
  • Journal Title: J Microbiol Methods. Volume: 152 Pages: 86-91
  • DOI: 10.1016/j.mimet.2018.07.017
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Surveillance evaluation of the standardization of assay values for serum total 25-hydroxyvitamin D concentration in Japan. (2018)
  • Author(s): Ihara H, Kiuchi S, Ishige T, Nishimura M, Matsushita K, Satoh M, Nomura F, Yamashita M, Kitajima I, Tsugawa N, Okano T, Hirota K, Miura M, Totani M, Hashizume N; Nutrition Division, Japan Society of Clinical Chemistry.
  • Journal Title: Ann Clin Biochem. Volume: Epub ahead of print Issue: 6 Pages: 0-0
  • DOI: 10.1177/0004563218765570
  • Peer Reviewed / Open Access
• [Journal Article] High-throughput genotyping of GC (vitamin D-binding protein) by melting analysis with locked nucleic acid-incorporating dual hybridization probe for improving mismatch discrimination. (2018)
  • Author(s): Ishige T, Satoh M, Itoga S, Nishimura M, Matsushita K, Nomura F.
  • Journal Title: Clin Chim Acta Volume: 487 Pages: 126-132
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  • Peer Reviewed / Open Access
• [Journal Article] Current Status of Proteomic Technologies for Discovering and Identifying Gingival Crevicular Fluid Biomarkers for Periodontal Disease. (2018)
  • Author(s): Tsuchida S, Satoh M, Takiwaki M, Nomura F
  • Journal Title: Int J Mol Sci Volume: 20 Issue: 1 Pages: 86-86
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  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Rosai-Dorfman disease and uveitis. (2018)
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• [Journal Article] An improved in-house lysis-filtration protocol for bacterial identification from positive blood culture bottles with high identification rates by MALDI-TOF MS. (2018)
  • Author(s): Tsuchida S, Murata S, Miyabe A, Satoh M, Takiwaki M, Matsushita K, Nomura F
  • Journal Title: Journal of microbiological methods Volume: 18 Pages: 1684-1688
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  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Assessment by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry of the Effects of Preanalytical Variables on Serum Peptidome Profiles Following Long-Term Sample Storage. (2018)
  • Author(s): Tsuchida S, Satoh M, Umemura H, Sogawa K, Takiwaki M, Ishige T, Miyabayashi Y, Iwasawa Y, Kobayashi S, Beppu M, Nishimura M, Kodera Y, Matsushita K, Nomura F
  • Journal Title: Proteomics. Clinical applications Volume: 15 Issue: 3 Pages: 47-53
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• [Journal Article] Variant in C-terminal region of intestinal alkaline phosphatase associated with benign familial hyperphosphatasaemia. (2018)
  • Author(s): Ishige T, Itoga S, Utsuno E, Nishimura M, Yoshikawa M, Kato N, Matsushita K, Yokosuka O, Nomura F
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  • DOI: 10.1136/jmedgenet-2017-104964
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• [Journal Article] One novel and two uncommon MEFV mutations in Japanese patients with familial Mediterranean fever: a clinicogenetic study (2017)
  • Author(s): Kishida D、Yazaki M、Nakamura A、Nomura F、Kondo T、他8名
  • Journal Title: Rheumatol Int Volume: 38 Issue: 1 Pages: 105-110
  • DOI: 10.1007/s00296-017-3886-z
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• [Journal Article] Accelerated oligosaccharide absorption and altered serum metabolites during oral glucose tolerance test in young Japanese with impaired glucose tolerance (2017)
  • Author(s): Miki Takashi、Lee Eun Young、Eguchi Akifumi、Sakurai Kenichi、Sawabe Yuji、Yoshida Tomohiko、Saito Keiko、Yokoh Hidetaka、Ishikawa Ko、Yokote Koutaro、Kuzuya Takeshi、Miki Eishi、Mori Chisato、Nomura Fumio
  • Journal Title: Journal of Diabetes Investigation Volume: 印刷中 Issue: 3 Pages: 512-521
  • DOI: 10.1111/jdi.12719
  • Peer Reviewed / Open Access
• [Journal Article] Cutaneous Rosai-Dorfman disease associated with uveitis (2017)
  • Author(s): Fukumoto T, Oka M, Masaki T, Sakaguchi M, Fukunaga A, Norose K, Sarayama Y, Imai H, Nishigori C
  • Journal Title: Eur J Dermatol Volume: 27 Issue: 1 Pages: 85-86
  • DOI: 10.1684/ejd.2016.2885
  • Peer Reviewed / Open Access
• [Journal Article] Utility of the cytochrome c oxidase subunit I gene for the diagnosis of toxoplasmosis using PCR. (2017)
  • Author(s): Feng X, Norose K, Li K, Hikosaka K.
  • Journal Title: J Microbiol Methods. Volume: 141 Pages: 82-86
  • DOI: 10.1016/j.mimet.2017.08.001
  • Peer Reviewed
• [Journal Article] FIR haplodeficiency promotes splicing to pyruvate kinase M2 (2017)
  • Author(s): A. Kimura, K.Kitamura, M. Satoh, K. Matsushita, et al.
  • Journal Title: Oncotarget Volume: 40 Issue: 40 Pages: 67955-67965
  • DOI: 10.18632/oncotarget.19061
  • Peer Reviewed / Open Access
• [Journal Article] Improved sensitivity of serum/plasma 1α,25-dihydroxyvitamin D quantification by DAPTAD derivatization (2017)
  • Author(s): Ishige Takayuki, Satoh Mamoru, Ogawa Shoujiro, Nishimura Motoi, Matsushita Kazuyuki, Higashi Tatsuya, Nomura Fumio
  • Journal Title: Clinica Chimica Acta Volume: 473 Pages: 173-179
  • DOI: 10.1016/j.cca.2017.08.033
  • Peer Reviewed / Open Access
• [Journal Article] Ubiquitination in Periodontal Disease: A Review (2017)
  • Author(s): Tsuchida S, Satoh M, Takiwaki M, Nomura F
  • Journal Title: Int J Mol Sci Volume: 18 Issue: 7 Pages: 1476-1476
  • DOI: 10.3390/ijms18071476
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Immune-complex level of cofilin-1 in sera is associated with cancer progression and poor prognosis in pancreatic cancer. (2017)
  • Author(s): Satoh M, Takano S, Sogawa K, Noda K, Yoshitomi H, Ishibashi M, Mogushi K, Takizawa H, Otsuka M, Shimizu H, Miyazaki M, Nomura F.
  • Journal Title: Cancer Sci. Volume: 印刷中 Issue: 4 Pages: 795-803
  • DOI: 10.1111/cas.13181
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 臍帯血移植後に多発性脳出血を来したトキソプラズマ関連中枢神経系血管炎 (2020)
  • Author(s): 太田 貴徳，楠元 大岳，上野稔幸、上原康文、杉尾 康浩，田宮 貞史、 彦坂 健児，野呂瀬 一美，大野 裕樹
  • Organizer: 第42回造血細胞移植学会総会
• [Presentation] Traditional Kampo formula (漢方) Scutellaria-Coptis (瀉心湯)is protective against Toxoplasma infection via synergistic immunomodulation. (2019)
  • Author(s): Shinjyo N, Hikosaka K, Yoshida H, Norose K.
  • Organizer: 第84回日本インターフェロン・サイトカイン学会学術集会
• [Presentation] PCR targeting the cytochrome c oxidase subunit 1 gene for clinical diagnosis of toxoplasmosis. (2019)
  • Author(s): Hikosaka K, Feng X, Koyama Y, Norose K.
  • Organizer: 4th Africa international biotechnology and biomedical conference, AIBBC 2019
  • Int'l Joint Research
• [Presentation] 多摩川河口域に飛来する野鳥の腸管寄生虫感染状況の解明 (2019)
  • Author(s): 彦坂健児、田中咲帆、馮 雪、篠田渉平、LiLi、Li Kexin, 野呂瀬 一美
  • Organizer: 第８８回日本寄生虫学会大会
• [Presentation] 自家末梢血幹細胞移植後に発症したトキソプラズマ症の検討－臨床所見およびST合剤予防投与の効果－ (2018)
  • Author(s): 北原茉莉、廣島由紀、植松望武、數本弘子、宍戸努、貝梅紘子、桐原健彦、武田航、植木俊充、住昌彦、上野真由美、市川直明、彦坂健児、野呂瀬一美、小林光
  • Organizer: 第40回 日本造血細胞移植学会総会
• [Presentation] 頚部リンパ節炎が長期間持続した急性トキソプラズマ症の一例 (2018)
  • Author(s): 松本佑慈、加勢田富士子、豊田一弘、村田昌之、野呂瀬一美、古庄憲浩
  • Organizer: 第92回日本感染症学会総会
• [Presentation] 造血幹細胞移植後にトキソプラズマ脳炎を発症したMDS/AMLの一例 (2018)
  • Author(s): 山村綾子、井上明威、徳永賢治、松野直史、野呂瀬一美、松岡雅雄
  • Organizer: 第40回造血細胞移植学会総会
• [Presentation] ディープシーケンスによるマラリア原虫のアトバコン耐性に関わる点変異の解析 (2018)
  • Author(s): 彦坂健児、馮 雪、本間 一、松崎素道、元岡大佑、中村昇太、野呂瀬一美、北 潔
  • Organizer: 第87回日本寄生虫学会大会
• [Presentation] Strongyloidiasis in Japan: Current status and perspectives (2018)
  • Author(s): 生野 博、石川 敬、野呂瀬一美
  • Organizer: 第87回日本寄生虫学会大会
• [Presentation] 眼トキソプラズマ症：マウスモデルにおける前房水中サイトカイン・ケモカイン解析―髄液および血清との比較 (2017)
  • Author(s): 李 可きん、馮 雪、彦坂健児、野呂瀬一美
  • Organizer: 第86回日本寄生虫学会大会
• [Presentation] シトクロムc酸化酵素サブユニット１遺伝子を標的としたPCRによる新規トキソプラズマ病診断法の検討 (2017)
  • Author(s): 馮 雪、Kexin Li, 野呂瀬一美、彦坂健児
  • Organizer: 第86回日本寄生虫学会大会
• [Presentation] 法医解剖前CTにおいて小腸内に条虫の存在が示唆された無鉤条虫症の1例 (2017)
  • Author(s): 星岡佑美、吉田真衣子、槇野陽介、彦坂健児、野呂瀬一美、岩瀬博太郎
  • Organizer: 第28回日本臨床寄生虫学会大会
• [Presentation] 全身性エリテマトーデス(SLE)再燃時に播種性トキソプラズマ症の診断に至った一例 (2017)
  • Author(s): 古矢裕樹、鈴木一正、彦坂健児、古田俊介、池田啓、中島裕史、野呂瀬一美
  • Organizer: 第28回日本臨床寄生虫学会大会
• [Presentation] Sulfamethoxazole-Trimethoprim(ST合剤)の脱感作により治療を継続できたトキソプラズマ脳炎(TE)の一例 (2017)
  • Author(s): 宮田順之、吉村幸浩、彦坂健児、野呂瀬一美、立川夏夫
  • Organizer: 第31回日本AIDS学会学術集会
• [Presentation] 本邦における糞線虫症の現状と展望 (2017)
  • Author(s): 生野 博、石川 敬、市村禎宏、野呂瀬一美
  • Organizer: Conbio 2017 生命科学系学会合同年次大会
• [Book] 今日の診断指針 (2020)
  • Author(s): 野呂瀬 一美
  • Total Pages: 2079
  • Publisher: 医学書院
  • ISBN: 9784260038089
• [Remarks] 千葉大学大学院・医学研究院 中核研究部門 生命機能治療学研究講座 感染生体防御学
  • URL: http://www.m.chiba-u.ac.jp/class/infection-hostdefense/
• [Remarks] 感染生体防御学
  • URL: http://www.m.chiba-u.ac.jp/class/infection-hostdefense/