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Functional Control by Modulating the Nucleic Acid Structure Based on Mismatch Base Pair Stabilization

Research Project

Project/Area Number 18105006
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Chemistry related to living body
Research InstitutionOsaka University

Principal Investigator

NAKATANI Kazuhiko  Osaka University, 産業科学研究所, 教授 (70237303)

Co-Investigator(Kenkyū-buntansha) DOHNO Chikara  大阪大学, 産業科学研究所, 准教授 (60420395)
HAGIHARA Masaki  大阪大学, 産業科学研究所, 助教 (40403000)
TAKEI Fumie  大阪大学, 産業科学研究所, 助教 (30252711)
ZHOU Daーyang  大阪大学, 産業科学研究所, 助教 (00324848)
Project Period (FY) 2006 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥113,100,000 (Direct Cost: ¥87,000,000、Indirect Cost: ¥26,100,000)
Fiscal Year 2010: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2009: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
Fiscal Year 2008: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2007: ¥45,890,000 (Direct Cost: ¥35,300,000、Indirect Cost: ¥10,590,000)
Fiscal Year 2006: ¥30,810,000 (Direct Cost: ¥23,700,000、Indirect Cost: ¥7,110,000)
Keywords核酸 / 光制御 / ミスマッチ / 分子認識 / DNAナノ構造 / ナノテクノロジー / DNA / 分子 / 高次構造 / 制御 / ヘアピンループ / 会合体 / 光異性化 / 核酸構造 / 機能発現 / 生体認識 / 機能化学 / 光応答 / RNA / 塩基対 / スイッチ / 機能制御
Research Abstract

We have established novel concept that small synthetic ligand, which selectively binds to DNA mismatch, could control higher order structures of DNA. DNA hybridization could be reversibly controlled by newly developed photoresponsive mismatch binding ligand, which allowed us to modulate various DNA-based functions depending on the ligand-specified DNA structures.

Report

(6 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Self-evaluation Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (58 results)

All 2011 2010 2009 2008 2007 2006 Other

All Journal Article (35 results) (of which Peer Reviewed: 25 results) Presentation (10 results) Book (2 results) Remarks (5 results) Patent(Industrial Property Rights) (6 results)

  • [Journal Article] Ligand-Assisted Complex of Two DNA Hairpin Loops.2011

    • Author(s)
      Hong, C.; Hagihara, M.; Nakatani, K.Angew.
    • Journal Title

      Chem.Int.Ed. 50

      Pages: 4390-4393

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ligand Inducible Assembly of a DNA Tetrahedron.2011

    • Author(s)
      Dohno, C.; Atsumi, H.; Nakatani, K.
    • Journal Title

      Chem.Commun. 47

      Pages: 3499-3501

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ligand-Assisted Complex of Two DNA Hairpin Loops.2011

    • Author(s)
      Hong, C.; Hagihara, M.; Nakatani, K.
    • Journal Title

      Angewandte Chemi International Edition

      Volume: (印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ligand Inducible Assembly of a DNA Tetrahedron.2011

    • Author(s)
      Dohno, C.; Atsumi, H.; Nakatani, K.
    • Journal Title

      Chemical Communication

      Volume: (印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Antisense-Induced Guanine Quadruplexes Inhibit Reverse Transcription by HIV-1 Reverse Transcriptase.2010

    • Author(s)
      Hagihara, M.; Yamauchi, L.; Seo, A.; Yoneda, K.; Senda, M.; Nakatani, K.
    • Journal Title

      J.Am.Chem.Soc. 132

      Pages: 11171-11178

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Non-covalent assembly of TEMPO radicals pair-wise embedded on a DNA duplex.2010

    • Author(s)
      Atsumi, H.; Maekawa, K.; Nakazawa, S.; Shiomi, D.; Sato, K.; Kitagawa, M.; Takui, T., Nakatani, K.
    • Journal Title

      Chem.Lett.(Editor's Choice) 39

      Pages: 556-557

    • NAID

      10027181105

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fluorescent Indicator-Displacement Assay for Ligand-RNA Interactions2010

    • Author(s)
      Zhang, J.; Umemoto, S.; Nakatani, K.
    • Journal Title

      J.Am.Chem.Soc. 132

      Pages: 3660-3661

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Programmed Assembly of Organic Radicals on DNA.2010

    • Author(s)
      Maekawa, K.; Nakazawa, S.; Atsumi, H.; Shiomi, D.; Sato, K.; Kitagawa, M.; Takui, T.; Nakatani, K.
    • Journal Title

      Chem.Commun. 46

      Pages: 1247-1249

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Non-covalent assembly of TEMPO radicals pair-wise embedded on a DNA duplex.2010

    • Author(s)
      Atsumi, H.; Maekawa, K.; Nakazawa, S.; Shiomi, D.; Sato, K.; Kitagawa, M.; Takui, T., Nakatani, K.
    • Journal Title

      Chemistry Letters

      Volume: (印刷中) Pages: 556-557

    • NAID

      10027181105

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Recognition of Mismatched Base Pairs in DNA2009

    • Author(s)
      Nakatani, K.
    • Journal Title

      Bull.Chem.Soc.Jpn.(総説) 82

      Pages: 1055-1069

    • NAID

      10025321391

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Secondary Structure-Inducible Ligand Fluorescence Coupled with PCR2009

    • Author(s)
      Takei, F.; Igarashi, M.; Hagihara, M.; Oka, Y.; Soya, Y.; Nakatani, K.
    • Journal Title

      Angew.Chem.Int.Ed. 48

      Pages: 7822-7824

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Light-Driven, Supramolecular Optical Switch2009

    • Author(s)
      Uno, S.; Dohno, C.; Bittermann, H.; Malinovskii, V.L.; Haner, R.; Nakatani, K.
    • Journal Title

      Angew.Chem.Int.Ed. 48

      Pages: 7362-7365

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Small molecule affecting the replication of trinucleotide repeat d(GAA)n2009

    • Author(s)
      He, H.; Hagihara, M.; Nakatani, K.
    • Journal Title

      Chem.Eur.J. 15

      Pages: 10641-10648

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Photoswitchable Unsymmetrical Ligand for DNA Hetero-Mismatches2009

    • Author(s)
      Dohno, C.; Yamamoto, T.; Nakatani, K.
    • Journal Title

      Eur.J.Org.Chem.

      Pages: 4051-4058

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] RNA Aptamers That Reversibly Bind Photoresponsive Azobenzene-Containing Peptides2009

    • Author(s)
      Hayashi, G.; Hagihara, M.; Nakatani, K.
    • Journal Title

      Chem.Eur.J. 15

      Pages: 424-432

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The effect of linker length on binding affinity of a photoawitchable molecular glue for DNA2009

    • Author(s)
      C.Dohno, et.al.
    • Journal Title

      Bioorg.Med.Chem. 17

      Pages: 2536-2543

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photoawitchable Unaymmetrical Ligand for DNA Hetero-Miamatches2009

    • Author(s)
      C.Dohno, et.al.
    • Journal Title

      Eur.J.Org.Chem.

      Pages: 4051-4058

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Light-Driven, Supramolecular Optical Switch2009

    • Author(s)
      S.Uno, et.al.
    • Journal Title

      Angew.Chem.Int.Ed. 48

      Pages: 7362-7365

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Small Molecule Affecting the Replication of Trinucleotide Repeat d(GAA)n.2009

    • Author(s)
      Hanging He, Masaki Hagihara, Kazuhiko Nakatani
    • Journal Title

      Chemistry-A European Journal 15巻

      Pages: 10641-10648

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Secondary Structure-Inducible Ligand Fluorescence Coupled with PCR2009

    • Author(s)
      Fumie Takei, Masako Igarashi, Masaki Hagihara, Yoshimi Oka, Yoehihiro Soya, Kazuhiko Nakatani
    • Journal Title

      Angewandte Chemie International Edition 48巻

      Pages: 7822-7824

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RNA Aptamers That Reversibly Bind Photoresponsive Azobenzene Containing Peptides2009

    • Author(s)
      Hayashi G.; Hagihara M.; Nakatani K.
    • Journal Title

      Chem. Eur. J. 15

      Pages: 424-432

    • Related Report
      2008 Self-evaluation Report
  • [Journal Article] DNA Labeling by Ligand Inducible Secondary Structure2008

    • Author(s)
      Peng T.; He H.; Hagihara M.; Nakatani K.
    • Journal Title

      ChemBioChem 9

      Pages: 1893-1897

    • Related Report
      2008 Self-evaluation Report
  • [Journal Article] Photoswitchable Molecular Glue for DNA2007

    • Author(s)
      Dohno, C.; Uno, S.; Nakatani
    • Journal Title

      K.J.Am.Chem.Soc. 129

      Pages: 11898-11899

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Photoregulation of a Peptide-RNA Interaction on a Gold Surface2007

    • Author(s)
      Hayashi, G.; Hagihara, M.; Dohno, C.; Nakatani, K.
    • Journal Title

      J.Am.Chem.Soc. 129

      Pages: 8678-8679

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Photoswitchable Molecular Glue for DNA2007

    • Author(s)
      Dohno C.; Uno S.; Nakatani K.
    • Journal Title

      J. Am. Chem. Soc. 129

      Pages: 11898-11899

    • Related Report
      2008 Self-evaluation Report
  • [Journal Article] Photoregulation of a Peptide RNA Interaction on a Gold Surface2007

    • Author(s)
      Hayashi G.; Hagihara M.; Dohno C.; Nakatani K.
    • Journal Title

      J. Am. Chem. Soc. 129

      Pages: 8678-8679

    • Related Report
      2008 Self-evaluation Report
  • [Journal Article] Photoswichable Molecular Glue for DNA2007

    • Author(s)
      Dohno, C., Uno, S., Nakatani, K
    • Journal Title

      J. Am. Chem. Soc 129

      Pages: 11898-11899

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photoregulation of a Peptide-RNA Interaction on a Gold Surface2007

    • Author(s)
      Hayashi, G., Hagihara, M., Dohno, C., Nakatani, K.
    • Journal Title

      J. Am. Chem. Soc 129

      Pages: 8678-8679

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of L-DNA-Tagged PCR Products by Surface Plasmon Resonance Imaging2007

    • Author(s)
      Hayashi, G., Hagihara, M., Kobori, A., Nakatani,K.
    • Journal Title

      ChemBioChem 8

      Pages: 169-169

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Evaluation of mismatch-binding ligands as inhibitors for Rev-RRE interaction2006

    • Author(s)
      Nakatani, K., Horie, S., Goto, Y., Kobori, A., Hagihara,S.
    • Journal Title

      Bioorg.Med.Chem. 14

      Pages: 5384-5384

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Mismatch binding ligands function as molecular glue for DNA2006

    • Author(s)
      Peng, T., Dohno, C., Nakatani,K
    • Journal Title

      Angew.Chem.Int.Ed. 45

      Pages: 5623-5623

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Control of DNA hybridization by photoswitchable molecular glue.

    • Author(s)
      Dohno, C.; Nakatani, K.
    • Journal Title

      Chem.Soc.Rev.(総説) in press.

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Bidirectional Control of Gold Nanoparticle Assembly by Turning On and Off DNA Hybridization with Thermally Degradable Molecular Glue

    • Author(s)
      Peng, T., Dohno, C., Nakatani, K.
    • Journal Title

      ChemBioChem (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Allele Specific C-Bulge Probes with One Unique Fluorescent Molecule Discriminate the Single Nucleotide Polymorphism in DNA

    • Author(s)
      Takei, F., Suda, H., Hagihara, M., Zhang, J., Kobori, A., Nakatani,K.
    • Journal Title

      Chem.Eur.J. (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Exploiting Small Molecule Binding to DNA for the Detection of Single-Nucleotide Mismatches and Their Base Environment

    • Author(s)
      Li, X., Song, H., Nakatani, K., Kraatz, H.-B.
    • Journal Title

      Anal.Chem. (in press)

    • Related Report
      2006 Annual Research Report
  • [Presentation] DNA・RNA に結合する小分子現状と課題2011

    • Author(s)
      中谷和彦
    • Organizer
      第13回生命化学研究会「分子で拓く生命化学」
    • Place of Presentation
      仙台(招待講演)
    • Year and Date
      2011-01-08
    • Related Report
      2010 Final Research Report
  • [Presentation] A Light-Driven, Light-switching DNA Device2010

    • Author(s)
      中谷和彦
    • Organizer
      DNA Photonics Symposium at the PacifiChem2010
    • Place of Presentation
      Honolulu, USA(招待講演)
    • Year and Date
      2010-12-17
    • Related Report
      2010 Final Research Report
  • [Presentation] Small molecules binding to the hairpin secondary structure of trinucleotide repeat sequences, Nucleic Acid Based2010

    • Author(s)
      中谷和彦
    • Organizer
      Therapeutics Symposium at the PacifiChem2010
    • Place of Presentation
      Honolulu, USA(招待講演)
    • Year and Date
      2010-12-16
    • Related Report
      2010 Final Research Report
  • [Presentation] Small molecules binding to the hairpin secondary structure of trinucleotide repeat sequences2010

    • Author(s)
      Kazuhiko Nakatani
    • Organizer
      Nucleic Acid Based Therapeutics Pacifichem, Honolulu
    • Place of Presentation
      Honolulu, Hawaii
    • Year and Date
      2010-12-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] ホルミル基含有修飾核酸によるDNAメチル化の識別2010

    • Author(s)
      柴田知範
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      近畿大学(大阪府)
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] RNA四重鎖構造を介した遺伝子発現制御2010

    • Author(s)
      萩原正規、中谷和彦
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      近畿大学 大阪
    • Year and Date
      2010-03-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] DNA cross-link generated by a novel modified DNA containing a formyl group2009

    • Author(s)
      柴田知範
    • Organizer
      The sixth International Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      高山市民文化会館(岐阜県)
    • Year and Date
      2009-09-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] 核酸二次構造安定性を大きく変化させる新規光応答性小分子リガンド2009

    • Author(s)
      堂野主税
    • Organizer
      第24回生体機能関連化学シンポジウム
    • Place of Presentation
      九州大学(福岡県)
    • Year and Date
      2009-09-13
    • Related Report
      2009 Annual Research Report
  • [Presentation] mRNAの非翻訳領域に存在する四重鎖構造2009

    • Author(s)
      萩原正規、中谷和彦
    • Organizer
      日本ケミカルバイオロジー研究会 第4回年会
    • Place of Presentation
      神戸市産業振興センター
    • Year and Date
      2009-05-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] Controlling Hybridization by Molecular Glue for DNA2008

    • Author(s)
      中谷和彦
    • Organizer
      17^<th> International Conference on Organic Synthesis
    • Place of Presentation
      Dejon, Korea
    • Related Report
      2008 Self-evaluation Report
  • [Book] ゲノム化学2007

    • Author(s)
      齋藤烈、杉山弘、中谷和彦編
    • Total Pages
      214
    • Publisher
      化学同人
    • Related Report
      2010 Final Research Report
  • [Book] ゲノム化学2007

    • Author(s)
      齋藤烈、杉山弘、中谷和彦
    • Publisher
      化学同人
    • Related Report
      2008 Self-evaluation Report
  • [Remarks] ホームページ

    • URL

      http://www.sanken.osaka-u.ac.jp/labs/rbc/

    • Related Report
      2010 Final Research Report
  • [Remarks] 第25回日本化学会学術賞 中谷和彦

    • Related Report
      2008 Self-evaluation Report
  • [Remarks] 第40回市村学術賞貢献賞 中谷和彦

    • Related Report
      2008 Self-evaluation Report
  • [Remarks] 第26回大阪科学賞 中谷和彦

    • Related Report
      2008 Self-evaluation Report
  • [Remarks]

    • URL

      http://www.sanken.osaka-u.ac.jp/labs/rbc

    • Related Report
      2008 Self-evaluation Report
  • [Patent(Industrial Property Rights)] 一塩基多型を検出する方法および一塩基多型を検出するための試薬キット2010

    • Inventor(s)
      中谷和彦、武井史恵、萩原正規
    • Industrial Property Rights Holder
      大阪大学
    • Industrial Property Number
      2010-054658
    • Filing Date
      2010-03-11
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] 核酸と被験物質との結合親和性を測定するための組成物及びその利用2008

    • Inventor(s)
      中谷和彦、張 錦華、梅本詩織、笹岡眞一、和崎隆博
    • Industrial Property Rights Holder
      大阪大学、日東化成株式会社
    • Industrial Property Number
      2009-519347
    • Filing Date
      2008-07-30
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] DNA二本鎖形成制御2006

    • Inventor(s)
      中谷和彦、Peng Tao、堂野主税
    • Industrial Property Rights Holder
      大阪大学
    • Industrial Property Number
      2006-162265
    • Filing Date
      2006-06-12
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] DNA二本鎖形成制御2006

    • Inventor(s)
      中谷ほか
    • Industrial Property Rights Holder
      大阪大学
    • Filing Date
      2006-06-12
    • Related Report
      2006 Annual Research Report
  • [Patent(Industrial Property Rights)] 核酸の増幅反応に用いるプライマ-の5'末端に結合して用いるDNA 断片およびその利用

    • Inventor(s)
      中谷和彦、武井史恵、萩原正規
    • Industrial Property Rights Holder
      大阪大学
    • Related Report
      2008 Self-evaluation Report
  • [Patent(Industrial Property Rights)] 核酸の増幅反応に用いる繰り返し配列結合プライマ-及びその利用

    • Inventor(s)
      中谷和彦、Peng Tao、萩原正規
    • Industrial Property Rights Holder
      大阪大学
    • Related Report
      2008 Self-evaluation Report

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Published: 2006-04-01   Modified: 2016-04-21  

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