Construction of Blood-Compatible Surface Based on Phase SeparationProperty of Glycoconjugated Polymer

• Project/Area Number: 18300158
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Hokkaido University
• Principal Investigator: KAKUCHI Toyoji Hokkaido University, 大学院・工学研究科, 教授 (80113538)
• Co-Investigator(Kenkyū-buntansha): SATOH Toshifumi 北海道大学, 大学院・工学研究科, 准教授 (80291235) ; SAKAI Ryosuke 北海道大学, 大学院・工学研究科, 助教 (90507196) ; 加我 晴生 独立行政法人産業技術総合研究所, ゲノムファクトリー研究部門, 主任研究員 (20356752) ; 鳴海 敦 山形大学, 大学院・理工学研究科, 助教 (60443975)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥17,390,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥2,190,000); Fiscal Year 2008: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2007: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2006: ¥7,900,000 (Direct Cost: ¥7,900,000)
• Keywords: リビングラジカル重合 / 糖鎖ポリマー / コーティング / 高分子ミセル / ミクロ相分離構造 / 抗血栓性 / 生体適合性 / マルトオリゴ糖 / ポリマーミセル / ミクロ不均質構造

Research Abstract

ヒト・動物由来の医用材料の使用は牛海綿状脳症 (BSE) やC型肝炎、さらに未知感染症に繋がるおそれがあるため、現在医用材料として用いることが強く規制されている。従って、動物由来の原料に依存しない安全な代替合成医用材料の開発が強く求められている。本研究では、糖鎖末端ポリマーを精密合成し、このポリマーを抗血栓性コーティング材料として応用することを目指した。抗血栓性に対して最も重要な因子である糖鎖ナノスポットの構造は任意に調節可能であることを明らかにした。

Research Products

• [Journal Article] Aqueous Self-Assembly of Polystyrene Chains End-Functionalized withs-Cyclodextrin (2009)
  • Author(s): Cristiano Giacomelli, Vanessa Schmidt,Jean-Luc Putaux, Atsushi Narumi, Toyoji Kakuchi, Redouane Borsali
  • Journal Title: Biomacromolecules 10(2) Pages: 449-453
  • Peer Reviewed
• [Journal Article] Aqueous Self-Assembly of Polystyrene Chains End-Functionalized with b -Cyclodextrin (2009)
  • Author(s): Cristiano Giacomelli
  • Journal Title: Biomacromolecules 10(2) Pages: 449-453
  • Peer Reviewed
• [Journal Article] Synthesis of Glycoconjugated Branched Macromolecular Architectures (2008)
  • Author(s): Atsushi Narumi, Toyoji Kakuchi
  • Journal Title: Polym. J 40(5) Pages: 383-397
  • NAID: 10021117332
  • Peer Reviewed
• [Journal Article] Chiropticalswitching system based on thehost-guest interaction between metalcations and poly(phenylacetylene) sbearing polycarbohydrate ionophore (2008)
  • Author(s): Otsuka, I., Sakai, R., Kakuchi, R., Satoh, T., Kakuchi, T
  • Journal Title: Eur. Polym. J. 44 Pages: 2971-2979
  • Peer Reviewed
• [Journal Article] Chiroptical switching system based on the host-guest interaction between metal cations and poly (phenylacetylene) sbearing polycarbohydrate ionophore (2008)
  • Author(s): lssei Otsuka
  • Journal Title: European Polymer Journal 44(9) Pages: 2971-2979
  • Peer Reviewed
• [Journal Article] Synthesis of glycoconjugated branched macromolecular architectures (2008)
  • Author(s): Atsushi Narumi
  • Journal Title: Polymer Journal 40(5) Pages: 38-397
  • NAID: 10021117332
  • Peer Reviewed
• [Journal Article] Thermochemical transformation of glucose to 1, 6-anhydroglucose in high-temperature steam (2008)
  • Author(s): Masahide Sasaki
  • Journal Title: Carbohydrate Research 343(5) Pages: 1185-1191
  • Peer Reviewed
• [Journal Article] Encapsulation-ReleaseProperty of AmphiphilicHyperbranched D-Glucan as a Unimolecular Reverse Micelle Polymer (2007)
  • Author(s): Kitajyo, Y., Imai, T., Sakai, Y., Tamaki,M., Tani, H., Takahashi, K., Narumi, A.,Kaga, H., Kaneko, N., Satoh, T. and Kakuchi, T
  • Journal Title: Polymer 48(5) Pages: 1237-1244
  • Peer Reviewed
• [Journal Article] A Unimolecular Nanocapsule. Encapsulation Propertyof Amphiphilic Polymer Based on Hyperbranched Polythreitol (2007)
  • Author(s): Kitajyo, Y., Nawa, Y., Tamaki, M., Tani,H., Takahashi, K., Kaga, H., Satoh and T, Kakuchi, T
  • Journal Title: Polymer 48(16) Pages: 4683-4690
  • Peer Reviewed
• [Journal Article] Synthesis, Characterization,and Functionalization ofHyperbranched Poly(3,4-epoxycyclohexanemethanol) (2007)
  • Author(s): Kitajyo, Y., Kinugawa, Y., Tamaki, M.,Kaga, H., Kaneko, N., Satoh, T. and Kakuchi, T
  • Journal Title: Macromolecules 40(26) Pages: 9313-9321
  • Peer Reviewed
• [Journal Article] Amphiphilic Hyperbranched Polysaccharide. Synthesis of Hyperbranched D-Glucan with L-Leucme Moiety as a Unimolecular Reverse Micelle Possessing an Encapsulation-Release Property toward Dye Molecules (2007)
  • Author(s): Yoshikazu Kitajyo
  • Journal Title: Polymer 48 Pages: 1237-1244
  • Peer Reviewed
• [Journal Article] Synthesis of Hyperbranched Carbohydrate Polymers by Ring-Opening Multibranching Polymerization of Anhydro Sugar (2007)
  • Author(s): Toshifumi Satoh
  • Journal Title: Macromolecular Bioscience 7 Pages: 999-1009
  • Peer Reviewed
• [Journal Article] Synthesis, Characterization, and Functionalization of Hyperbranched Poly(3,4-epoxycyclohexanemethanol) (2007)
  • Author(s): Yoshikazu Kitajyo
  • Journal Title: Macromolecules 40 Pages: 9313-9321
  • Peer Reviewed
• [Journal Article] Synthesisof polystyrene microgel with glucose ashydrophilic segment via controlledfree-radical polymerization (2006)
  • Author(s): Narumi, A., Kaga, H., Miura, Y., Satoh,T., Kaneko, N., Kakuchi, T
  • Journal Title: Polymer 47(7) Pages: 2269-2273
  • Peer Reviewed
• [Journal Article] Polystyrene Microgel Amphiphiles with Maltohexaose.Synthesis, Characterization, and Potential Applications (2006)
  • Author(s): Narumi, A., Kaga, H., Miura, Y.,Otsuka, I., Satoh, T., Kaneko, N., Kakuchi, T
  • Journal Title: Biomacromolecules 7(5) Pages: 1496-1501
  • Peer Reviewed
• [Journal Article] End-functionalization of polystyrene by malto-oligosaccharide generating aggregation-tunable polymeric reverse micelle (2006)
  • Author(s): Narumi, A., Miura, Y., Otsuka, I.,Yamane, S., Kitajyo, Y., Satoh, T.,Hirao, A., Kaneko, N., Kaga, H., Kakuchi, T
  • Journal Title: Journal of Polymer Science, Part A : Polymer Chemistry 44(16) Pages: 4864-4879
  • Peer Reviewed
• [Journal Article] Glycoconjugated polymer : synthesis and characterization of poly(vinylsaccharide)-block-polystyrene-block-poly(vinyl saccharide) as an amphiphilic ABA triblock copolymer (2006)
  • Author(s): Narumi, A., Otsuka, I., Matsuda, T.,Miura, Y., Satoh, T., Kaneko, N., Kaga,H., Kakuchi, T.
  • Journal Title: J. Polym. Sci., Part A : Polym. Chem 44(13) Pages: 3978-3985
  • Peer Reviewed
• [Journal Article] Polystyrene Microgel Amphiphiles with Maltohexaose as Hydrophilic Segment. Synthesis, Characterization, and Potential Applications (2006)
  • Author(s): Atsushi Narumi
  • Journal Title: Biomacromolecules 7・5 Pages: 1496-1501
• [Journal Article] End-Functionalization of Polystyrenes by Malto-oligosaccharides Generating Aggregation-Tunable Polymeric Reverse Micelle (2006)
  • Author(s): Atsushi Narumi
  • Journal Title: J. Polym. Sci. Part A : Polym. Chem. 44・16 Pages: 4864-4879
• [Journal Article] Glycoconjugated Polymer : Synthesis and Characterization of Poly(vinyl saccharide)-block-polystyrene-block-poly(vinyl saccharide) as an Amphiphilic ABA Triblock Copolymer (2006)
  • Author(s): Atsushi Narumi
  • Journal Title: J. Polym. Sci. Part A : Polym. Chem. 44・13 Pages: 1117-1124
• [Journal Article] "Synthesis of Polystyrene Microgel with Glucose as Hydrophilic Segment via Controlled Radical Polymerization (2006)
  • Author(s): Atsushi Narumi
  • Journal Title: Polymer 47・7 Pages: 2269-2273
• [Journal Article] Synthesis of Well-defined Syndiotactic Poly(Methyl Methacrylate) with Low Temperature Atom Transfer Radical Polymerization in Fluoroalcohol (2006)
  • Author(s): Yutaka Miura
  • Journal Title: J. Polym. Sci. Part A : Polym. Chem. 44・4 Pages: 1436-1446
• [Journal Article] Synthesis and Photocrosslinking Reaction of N-Allylcalbamoylmenthyl Cellulose leading to Hydrogel (2006)
  • Author(s): Xiande Shen
  • Journal Title: Polym. Bull. 56・2-3 Pages: 137-143
• [Presentation] マルトオリゴ糖を側鎖に有するポリフェニルアセチレンのキラル特性 (2008)
  • Author(s): Barasa Eric Barack
  • Organizer: 第57回高分子学会年次大会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2008-05-28
• [Presentation] ラクトースを外殻に持つ両親媒性ハイパーブランチ糖鎖高分子の合成と分子捕捉能 (2008)
  • Author(s): 佐藤敏文
  • Organizer: 第57回高分子学会年次大会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2008-05-28
• [Presentation] Synthesis and characterization of poly (p-phenylene ethynylene) bearing malto-hexaose (2008)
  • Author(s): R. Konosu・T. Hongo・I. Otsuka・A. Narumi・T. Satoh・A. Deffieux・A. Hirao・T. Kakuchi
  • Organizer: 235th American Chemical Society National Meeting
  • Place of Presentation: Morial Convention Center-Hall A, New Orleans, LA, USA
  • Year and Date: 2008-04-06
• [Presentation] Synthesis and characterization of poly (p-phenylene ethynylene) bearing malto-hexaose (2008)
  • Author(s): Ryo Konosu
  • Organizer: 235th American Chemical Society National Meeting
  • Place of Presentation: Mori al Convention Center New Orleans, LA, USA
  • Year and Date: 2008-04-06
• [Presentation] Synthesis of hyperbranched Carbohydrate Polymer, Taiwan-Japan International Symposium on Organic Chemistry and Molecular Science (2008)
  • Author(s): Toyoji Kakuchi
  • Organizer: National Taipei University ofTechnology Taipei
  • Place of Presentation: Taiwan
• [Presentation] マルトオリゴ糖を側鎖に有するポリフェニルアセチレンの合成とキラル特性 (2007)
  • Author(s): 本郷孝剛・大塚一世・堺井亮介・鳴海敦・佐藤敏文・Alain Deffieux・平尾明・覚知豊次
  • Organizer: 第56回高分子討論会
  • Place of Presentation: 名古屋工業大学
• [Presentation] マルトヘキサオースを側鎖に有するπ共役高分子の合成とレクチン認識能 (2007)
  • Author(s): 鴻巣亮・本郷孝剛・大塚一世・鳴海敦・佐藤敏文・Alain Deffieux・平尾明・覚知豊次
  • Organizer: 第56回高分子討論会
  • Place of Presentation: 名古屋工業大学