Development of novel sensing method using gold-nanorod monolayer films

• Project/Area Number: 18310069
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Nanostructural science
• Research Institution: Kyushu University
• Principal Investigator: NIIDOME Yasuro Kyushu University, 大学院・工学研究院・応用化学部門(分子), 准教授 (50264081)
• Co-Investigator(Kenkyū-buntansha): HIDEYA Kawasaki 関西大学, 化学生命工学部, 准教授 (50322285)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥16,880,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥1,680,000); Fiscal Year 2008: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2007: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2006: ¥9,600,000 (Direct Cost: ¥9,600,000)
• Keywords: 金ナノロッド / 表面プラズモン / プラズモンセンサー / 単粒子薄膜 / 薄膜センサー / 配向制御

Research Abstract

リン脂質修飾金ナノロッドを凝集させ、さらに乾燥させた後に、溶液中に再分散できる条件を見いだした。リン脂質修飾金ナノロッドには過剰な界面活性剤や高分子を含まず、最小限のリン脂質とカチオン性界面活性剤でその分散安定性が保たれている状態である。このため、乾燥した金ナノロッドは金属光沢を示した。この状態で金ナノロッドを冷蔵庫に保管することで不可逆な凝集を抑制できることがわかった。これは新しい金属ナノ粒子の保管方法・表面修飾方法として他に例を見ないものであり、今後の学術的あるいは応用に向けた研究展開が期待される。
液液界面を利用した単粒子薄膜作製法により、金ナノロッドの凝集状態を制御できることを明らかにするとともに、高分子電解質で表面修飾した金ナノロッドをガラス基板に静電相互作用で固定する方法を確立した。基板に固定した金ナノロッドは明確な表面プラズモンバンドを示すことから、ナノロッドが孤立状態で固定できることが明らかになった。この金ナノロッドに抗体を固定し、更に抗原となるタンパク質を結合させた。それぞれのステップで表面プラズモンバンドの変化をモニターしたところ、抗体・抗原の吸着に伴って再現性の良いピークシフトが得られることを明らかにした。この実験によって得られたピークシフトは従来の報告にない大きなものであり、金ナノロッドセンサーの実用化に向けた大きな技術的進歩が得られた。また、検出感度も10-10 M のオーダーの下限を有しており、今後の最適化によって従来のセンサーを上回る検出感度を実現できることが明らかになった。

Research Products

• [Journal Article] Rapid Formation of Silver Shells on Gold Nanorods in a Micellar Solution of Hexadecyltrimethylammonium Chloride (2009)
  • Author(s): Yoshifumi Okuno, Koji Nishioka, Naotoshi Nakashima, Yasuro Niiodme
  • Journal Title: Chemistry Letters, 2009 Vol.38, No.1 Pages: 60-61
• [Journal Article] Photothermal Reshaping of Gold Nanorods Depends on the Passivating Layers of the Nanorod Surfaces (2008)
  • Author(s): Yukichi Horiguchi, Kanako Honda, Yuichi Kato, Naotoshi Nakashima, Yasuro Niiodme
  • Journal Title: Langmuir, 2008 Vol.24, No.20 Pages: 12026-12031
• [Journal Article] In vivo monitoring of intravenously injected gold nanorods using near-infrared light (2008)
  • Author(s): Takuro Niidome, Yasuyuki Akiyama, Kohei Shimoda, Takahito Kawano, Takeshi Mori, Yoshiki Katayama, and Yasuro Niiodme
  • Journal Title: Small, 2008 Vol.4, No.7 Pages: 1001-1007
• [Journal Article] Photoinduced Release of Oligonucleotide-conjugated Silica-coated Gold Nanorods Accompanied by Moderate Morphological Changes (2008)
  • Author(s): Yukichi Horiguchi, Shuji Yamashia, Takuro Niidome, Naotoshi Nakashima, Yasuro Niiodme
  • Journal Title: Chemistry Letters, 2008 Vol.37, No.7 Pages: 718-719
  • NAID: 10021082084
• [Journal Article] Surface Modification of Gold Nanorods using Layer-by-Layer Technique for Cellular Uptake (2008)
  • Author(s): Hironobu Takahashi, Takuro Niidome, Takahito Kawasno, Yasuro Niiodme, and Sunao Yamada
  • Journal Title: Journal of Nanoparticle Research, 2008 Vol.10, No.1 Pages: 221-228
• [Journal Article] Formation of Gold Nanorod-Myoglobin Aggregates by Electrostatic Interactions and Their Photochemical Properties (2008)
  • Author(s): Rumiko Kitagawa, Kanako Honda, Hirofumi Kawazumi, Yasuro Niiodme, Naotoshi Nakashima, and Sunao Yamada
  • Journal Title: Japanese Journal of Applied Physics, 2008 Vol.47, No.2 Pages: 1374-1376
• [Journal Article] Photoinduced Release of Oligonucleotide-conjugated Silica-coated Gold Nanorods Accompanied by Moderate Morphological Changes (2008)
  • Author(s): Yukichi Horiguchi, Shuji Yamashia, Takuro Niidome, Naotoshi Nakashima, Yasuro Niidome
  • Journal Title: Chemistry Letters 37 Pages: 718-719
  • NAID: 10021082084
  • Peer Reviewed
• [Journal Article] In vivo monitoring of intravenously injected gold nanorods using near-infrared light (2008)
  • Author(s): Takuro Niidome, Yasuyuki Akiyama, Kohei Shimoda, Takahito Kawano, Takeshi Mori, Yoshiki Katayama, Yasuro Niidome
  • Journal Title: Small 4 Pages: 1001-1007
  • Peer Reviewed
• [Journal Article] Photothermal Reshaping of Gold Nanorods Depends on the Passivating Layers of the Nanorod Surfaces (2008)
  • Author(s): Yukichi Horiguchi, Kanako Honda, Yuichi Kato, Naotoshi Nakashima, Yasuro Niidome
  • Journal Title: Langmuir 24 Pages: 12026-12031
  • Peer Reviewed
• [Journal Article] Photochemical Reactions of Ketones to Synthesize Gold Nanorods (2007)
  • Author(s): Koji Nishioka, Yasuro Niiodme, Sunao Yamada
  • Journal Title: Langmuir, 2007 Vol.23, No.20 Pages: 10353-10356
• [Journal Article] Yasuro Niiodme, Kanako Honda, Keisuke Higashimoto, Hirofumi Kawazumi, Sunao Yamada, Naotoshi Nakashima, Yoshihiro Sasaki, Yoshihiko Ishida, Jun-ichi Kikuchi (2007)
  • Author(s): Surface Modification of Gold Nanorods with Synthetic Cationic Lipids
  • Journal Title: Chemical Communications, 2007 No.36 Pages: 3777-3779
• [Journal Article] Expression of Plasmid DNA Released from DNA Conjugates of Gold Nanorods (2007)
  • Author(s): Yukichi Horiguchi, Takuro Niidome, Sunao Yamada, Naotoshi Nakashima, and Yasuro Niiodme
  • Journal Title: Chemistry Letters, 2007 Vol.36, No.7 Pages: 952-953
  • NAID: 10019746014
• [Journal Article] Extraction of Hexadecyltrimethylammonium Bromide from Gold Nanorod Solutions : Adsorption of Gold Nanorods on Anionic Glass Surfaces (2007)
  • Author(s): Kanako Honda, Hirofumi Kawazumi, Sunao Yamada, Naotoshi Nakashima, and Yasuro Niiodme
  • Journal Title: Transaction of Material Research Society of Japan, 2007 Vol.32, No.2 Pages: 421-424
• [Journal Article] Stable Incorporation of Gold Nanorods into N-isopropylacrylamide Hydrogels and Their Rapid Shrinkage Induced by Near-IR Laser Irradiation (2007)
  • Author(s): Atsushi Shiotani, Takeshi Mori, Takuro Niidome, Yasuro Niiodme, and Yoshiki Katayama
  • Journal Title: Langmuir, 2007 Vol.23, No.7 Pages: 4012-4018
• [Journal Article] Photochemical Reactions of Ketones to Synthesize Gold Nanorods (2007)
  • Author(s): Koji Nishioka, Yasuro Niidome, Sunao Yamada
  • Journal Title: Langmuir 23 Pages: 10353-10356
  • Peer Reviewed
• [Journal Article] Surface Modification of Gold Nanorods with Synthetic Cationic Lipids (2007)
  • Author(s): Yasuro Niidome, Kanako Honda, 他7名
  • Journal Title: Chemical Communications Pages: 3777-3779
  • Peer Reviewed
• [Journal Article] Expression of Plasmid DNA Released from DNA Conjugates of Gold Nanorods (2007)
  • Author(s): Yukichi Horiguchi, Yasuro Niidome, 他3名
  • Journal Title: Chemistry Letters 36 Pages: 952-953
  • NAID: 10019746014
  • Peer Reviewed
• [Journal Article] Extraction of Hexadecyltrimethylammonium Bromide from Gold Nanorod Solutions : Adsorption of Gold Nanorods on Anionic Glass Surfaces (2007)
  • Author(s): K.Honda, H.Kawazumi, S.Yamada, N.Nakashima, Y.Niidome
  • Journal Title: Transaction of Material Research Society of Japan (In press)
  • NAID: 130007922763
• [Journal Article] Surface Modification of Gold Nanorods using Layer-by-Layer Technique for Cellular Uptake (2007)
  • Author(s): H.Takahashi, T.Niidome, T.Kawasno, Y.Niidome, S.Yamada
  • Journal Title: Journal of Nanoparticle Research (In press)
• [Journal Article] Spatio-Selective Surface Modification of Glass Assisted by Laser-Induced Deposition of Gold Nanoparticles (2006)
  • Author(s): Hironobu Takahashi, Yasuro Niiodme, Hideyuki Hisanabe, Keita Kuroiwa, and Sunao Yamada
  • Journal Title: Thin Solid Films, 2006 Vol.515, Nos.4-5 Pages: 1618-1622
• [Journal Article] Pulsed-Laser Induced Flocculation of Carbon Nanotubes Solubilized by an Anthracene-Carrying Polymer (2006)
  • Author(s): Kaori Narimatsu, Yasuro Niiodme, and Naotoshi Nakashima
  • Journal Title: Chemical Physics Letters, 2006 Vol.429, No.4-6 Pages: 488-491
• [Journal Article] 金ナノ粒子レーザ誘起固定化の制御とセンシングへの応用 (2006)
  • Author(s): 本夛加菜子、新留 康郎、河済博文、山田淳
  • Journal Title: 分析化学、2006 Vol.55, No.9 Pages: 675-679
• [Journal Article] PEG-Modified Gold Nanorods with a Stealth Character for in vivo application (2006)
  • Author(s): Takuro Niidome, Masato Yamagata, Yuri Okamoto, Yasuyuki Akiyama, Hironobu Takahashi, Takahito Kawano, Yoshiki Katayama, and Yasuro Niiodme
  • Journal Title: Journal of Controlled Release, 2006 Vol.114, No.3 Pages: 343-347
• [Journal Article] Photothermal Reshaping of Gold Nanorods Prevents Further Cell Death (2006)
  • Author(s): Hironobu Takahashi, Takuro Niidome, Ayuko Nariai, Yasuro Niiodme, and Sunao Yamada
  • Journal Title: Nanotechnology, 2006 Vol.17, No.17 Pages: 4431-4435
• [Journal Article] End-to-End Assemblies of Gold Nanorods Adsorbed on a Glass Substrate Modified with Polyanion Polymers (2006)
  • Author(s): Kanako Honda, Yasuro Niiodme, Naotoshi Nakashima, Hirofumi Kawazumi, and Sunao Yamada
  • Journal Title: Chemistry Letters, 2006 Vol.35, No.8 Pages: 852-853
• [Journal Article] Deposition of Indium Oxide Thin Films Assisted by Gold Nanoparticles in Cyclohexane (2006)
  • Author(s): Yasuro Niiodme, Hideyuki Hisanabe, Takahiro Kawasawa, and Sunao Yamada
  • Journal Title: Thin Solid Films, 2006 Vol.513, Nos.1-2 Pages: 60-63
• [Journal Article] Heat-induced Morphological Control of Gold Nanoparticle Films for Surface-Enhanced Raman Scattering (SERS) Measurements (2006)
  • Author(s): Mototsugu Suzuki, Yasuro Niiodme, and Sunao Yamada
  • Journal Title: Colloid and Surfaces A, 2006 Vol.284-285 Pages: 388-394
• [Journal Article] Two-dimensional Aggregates of Gold Nanorods Prepared at a Hexane-Water Interface in the Presence of Additional Amphiphilic Molecules (2006)
  • Author(s): Yasuro Niiodme, Minami Yamaguchi, Hironobu Takahashi, and Sunao Yamada
  • Journal Title: Transactions of the Materials Research Society of Japan, 2006 Vol.30, No.2 Pages: 571-574
• [Journal Article] Gold Nanorod-Sensitized Cell Death : Microscopic Observation of Single Living Cells Irradiated by Pulsed Near-Infrared Laser Light in the Presence of Gold Nanorods (2006)
  • Author(s): Hironobu Takahashi, Takuro Niidome, Ayuko Nariai, Yasuro Niidome, and Sunao Yamada
  • Journal Title: Chemistry Letters, 2006 Vol.35, No.5 Pages: 500-501
  • NAID: 10017409831
• [Journal Article] Stabilizing of plasmid DNA in vivo by PEG-modified cationic gold nanoparticles and the gene expression assisted with electrical pulses (2006)
  • Author(s): Takahiro Kawano, Masato Yamagata, Hironobu Takahashi, Yasuro Niiodme, Sunao Yamada, Yoshiki atayama, and Takuro Niidome
  • Journal Title: Journal of Controlled Release, 2006 Vol.111, No.3 Pages: 382-389
• [Journal Article] End-to-End Assemblies of Gold Nanorods Adsorbed on a Glass Substrate Modified with Polyanion Polymers (2006)
  • Author(s): K.Honda, Y.Niidome, N.Nakashima, H.Kawazumi, S.Yamada
  • Journal Title: Chemistry Letters 35・7 Pages: 832-853
  • NAID: 10018187327
• [Journal Article] 金ナノ粒子レーザ誘起固定化の制御とセンシングへの応用 (2006)
  • Author(s): 本多加菜子, 新留康郎, 河済博文, 山田淳
  • Journal Title: 分析化学 55・9 Pages: 675-679
• [Journal Article] PEG-Modified Gold Nanorods with a Stealth Character for in vivo application (2006)
  • Author(s): T.Niidome, M.Yamagata, Y.Okamoto, Y.Akiyama, H.Takahashi, T.Kawano, Y.Katayama, Y.Niidome
  • Journal Title: Journal of Controlled Release 114・3 Pages: 343-347
• [Journal Article] Two-dimensional Aggregates of Gold Nanorods Prepared at a Hexane-Water Interface in the Presence of Additional Amphiphilic Molecules (2006)
  • Author(s): Y.Niidome, M.Yamaguchi, H.Takahashi, S.Yamada
  • Journal Title: Transactions of the Materials Research Society of Japan 30・2 Pages: 571-574
• [Presentation] Photoinduced Release of Oligonucleotide from Silica-coated Gold Nanorods (2008)
  • Author(s): Yasuro Niiodme、Yukichi Horiguchi、Takuro Niidome
  • Organizer: International Symposium on Molecular and System Life Sciences
  • Year and Date: 2008-12-11
• [Presentation] Gold Nanorod-modified Substrates for Localized Plasmon Resonance Sensing of Biomolecules (2008)
  • Author(s): Yasuro Niiodme、Kanako Honda、Yuki Nakamura、Naotoshi Nakashima
  • Organizer: MRS 2008 Fall Meeting
  • Year and Date: 2008-12-02
• [Presentation] Preparation and application of anisotropic metal nanoparticles (2008)
  • Author(s): Yasuro Niiodme
  • Organizer: 理研シンポジウム
  • Year and Date: 2008-11-10
• [Presentation] Preparation of Dried Gold nanorods by the Addition of 6-Amino-1-hexanethiol Hydrochloride (2008)
  • Author(s): Kanako Honda、Hirofumi Kawazumi、Naotoshi Nakashima、Yasuro Niiodme
  • Organizer: 82nd Colloid and Surface Science Symposium
  • Year and Date: 2008-06-15
• [Presentation] Pulsed-Laser Triggered Release of DNA from Gold Nanorod-DNA Conjugates (2008)
  • Author(s): Yukichi Horiguchi、Takuro Niidome、Naotoshi Nakashima、Yasuro Niiodme
  • Organizer: 82nd Colloid and Surface Science Symposium
  • Year and Date: 2008-06-15
• [Presentation] Photochemical synthesis of gold nanorods (2008)
  • Author(s): Yasuro Niiodme、Koji Nishioka
  • Organizer: 82nd Colloid and Surface Science Symposium
  • Year and Date: 2008-06-15
• [Presentation] Photochemical synthesis of gold nanorods (2008)
  • Author(s): Yasuro Niidome
  • Organizer: 82nd Colloid and Surface Science Symposium
  • Place of Presentation: North Calorina, 米国
  • Year and Date: 2008-06-15
• [Presentation] Photoinduced Release of Plasmid DNA from Gold nanorod Conjugates (2007)
  • Author(s): Yukichi Horiguchi
  • Organizer: MRS 2007 Fall Metting
  • Year and Date: 2007-11-27
• [Presentation] Distribution and Aggregation of Gold Nanorods in Living Cells (2007)
  • Author(s): Niidome Yasuro
  • Organizer: Materials Research Society, 2007 Fall Meeting
  • Place of Presentation: Boston, 米国
  • Year and Date: 2007-11-26
• [Presentation] Formation of Gold Nanorod-Myoglobin and-Nanorod Aggregates by Electrostatic Interactions and Photochemical Properties
  • Author(s): Kanako Honda 、Rumiko Kitagawa、Yasuro Niiodme、Sunao Yamada
  • Organizer: Fourth International Conference on Molecular Electronics and Bioelectronics
• [Book] 金ナノテクノロジー : その基礎と応用(春田正毅編集、金ナノロッドの合成) (2009)
  • Author(s): 新留康郎
  • Publisher: シーエムシー出版
• [Book] ナノ粒子の創成と応用展開(米澤徹編集、金ナノロッドの合成と構造) (2008)
  • Author(s): 新留康郎
  • Publisher: フロンティア出版
• [Book] 5章, 金ナノロッドの合成 (2008)
  • Author(s): 新留 康郎
  • Total Pages: 9
  • Publisher: シーエムシー出版
• [Book] Handai Nanophotonics Vol.3, Chapter 20, "Gold nanorods : application of bioscience and medicine", Elsevier (2007)
  • Author(s): Yasuro Niiodme and Takuro Niidome
• [Book] 第5版実験化学講座、(20-1巻、12章4節、ナノ粒子分析試薬) (2007)
  • Author(s): 山田 淳、新留 康郎
  • Publisher: 丸善
• [Book] Chapter 20, Gold nanorods: application of bioscience and medicine, Handai Nanophotonics (2007)
  • Author(s): Yasuro Niidome, Takuro Niidome
  • Total Pages: 10
  • Publisher: Elsevier
• [Book] プラズモンナノ材料の設計と応用技術(山田 淳監修、第15章金ナノロッドのフォトニクス) (2006)
  • Author(s): 新留康郎
  • Publisher: シーエムシー出版
• [Book] Spectroscopy and Photoreactions of Gold Nanorods in Living Cells and Organisms(Yasuro Niiodme and Takuro Niidome (Chapter review))
  • Author(s): Molecular Nano Dynamics, Ed. H. Fukumura, Wiley
  • Publisher: (in press.)
• [Remarks]
  • URL: http://www.nanorod.net
• [Remarks]
  • URL: http://www.nanorod.net
• [Remarks]
  • URL: http://www.nanorod.net
• [Patent(Industrial Property Rights)] 金微粒子とその製造方法、およびその用途 (2008)
  • Inventor(s): 新留琢郎、新留 康郎、大賀 晃、森 健、片山佳樹
  • Industrial Property Rights Holder: 国立大学法人九州大学、三菱マテリアル株式会社、大日本塗料株式会社
  • Industrial Property Number: 2008-171300
  • Filing Date: 2008-06-30
• [Patent(Industrial Property Rights)] 分析用チップとその製造方法およびその分析方法 (2008)
  • Inventor(s): 新留 康郎、本夛加菜子、新留琢郎
  • Industrial Property Rights Holder: 新留 康郎、新留琢郎、三菱マテリアル、大日本塗料
  • Industrial Property Number: 2008-118414
  • Filing Date: 2008-04-20
• [Patent(Industrial Property Rights)] 金属ナノ材料の製造方法およびそれにより得られる金属ナノ材料 (2008)
  • Inventor(s): 新留 康郎、西岡宏司、奥野嘉文
  • Industrial Property Rights Holder: 国立大学法人九州大学
  • Industrial Property Number: 2008-023265
  • Filing Date: 2008-02-01
• [Patent(Industrial Property Rights)] 金属ナノ材料の製造方法およびそれにより得られる金属ナノ材料 (2008)
  • Inventor(s): 新留康郎, 奥野嘉文, 西岡宏司
  • Industrial Property Rights Holder: 九州大学
  • Industrial Property Number: 2008-023265
  • Filing Date: 2008-11-06
• [Patent(Industrial Property Rights)] 金属微粒子の表面処理方法とその金属微粒子の乾燥体ないし分散液 (2007)
  • Inventor(s): 新留 康郎、本夛加菜子
  • Industrial Property Rights Holder: 九州大学、三菱マテリアル、大日本塗料
  • Industrial Property Number: 2007-302938
  • Filing Date: 2007-11-22
• [Patent(Industrial Property Rights)] 金属微粒子の表面処理方法とその金属微粒子の乾燥体ないし分散液 (2007)
  • Inventor(s): 新留康郎, 本歩夛加菜子
  • Industrial Property Rights Holder: 九州大学、三菱マテリアル、大日本塗料
  • Industrial Property Number: 2007-302938
  • Filing Date: 2007-11-22