Analysis of the mechanism of metabolic control of nitrate inhibition on nodules and autoregulation of nodulation in soybean.

• Project/Area Number: 18380049
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Niigata University
• Principal Investigator: OHYAMA Takuji Niigata University, 自然科学系, 教授 (30152268)
• Co-Investigator(Kenkyū-buntansha): SUEYOSHI Kuni 新潟大学, 自然科学系, 准教授 (10216278) ; OHTAKE Norikuni 新潟大学, 自然科学系, 准教授 (50313507)
• Project Period (FY): 2006 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥16,570,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥1,470,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2006: ¥10,200,000 (Direct Cost: ¥10,200,000)
• Keywords: ダイズ / 根粒 / 窒素固定 / 硝酸 / 葉 / 根 / 根粒超着生変異株 / 根粒形成自己制御 / 光合成産物 / アンモニア / グルタミン / 尿素

Research Abstract

ダイズの根粒生長と窒素固定活性の急速かつ可逆的な阻害が、アンモニア、尿素、グルタミンを培養液に投与した場合にも硝酸と同様に認められた。窒素化合物による根粒生長阻害は、^<13>Cと^<15>Nを用いた実験から光合成産物の根粒への移動低下が原因であると推定された。初生葉の葉柄から各種化合物を投与する実験で、グルタミンを投与したときに顕著な根粒形成の抑制が認められたことから、グルタミンが制御シグナルである可能性が示唆された。

Research Products

• [Journal Article] The autoregulation of nodulation mechanism is related to leaf development (2008)
  • Author(s): Sayuri Ito, Taichi Kato, Norikuni Ohtake, Kuni Sueyoshi, Takuji Ohyama
  • Journal Title: Plant Cell Physiology 49 Pages: 121-125
  • NAID: 10027495411
  • Peer Reviewed
• [Journal Article] The autoregulation of nodulation mechanism is related to leaf development. (2008)
  • Author(s): Sayuri ITO, Taichi KATO, Norikuni OHTAKE, Kuni SUEYOSHI and Takuji OHYAMA
  • Journal Title: Plant Cell Physiology 49 Pages: 121-125
  • NAID: 10027495411
  • Peer Reviewed
• [Journal Article] Characteristics of initial growth of hypernodulation soybean mutants, NOD1-3, NOD2-4, and NOD3-7, affected by inoculation of bradyrhizobia and nitrate supply (2007)
  • Author(s): Sayuri Ito, Norikuni Ohtake, Kuni Sueyoshi, Takuji Ohyama
  • Journal Title: Soil Sci. Plant Nutr 53 Pages: 66-71
  • NAID: 110006532365
  • Peer Reviewed
• [Journal Article] Characteristics of initial growth of hypernodulation soybean mutants, NOD1-3, NOD2-4 and NOD3-7, affected by inoculation of bardyrhizobia and nitrate supply. (2007)
  • Author(s): Sayuri ITO, Norikumi OHTAKE, Kuni SUEYOSHI and Takuji OHYAMA
  • Journal Title: Soil Sci. Plant Nuutr. 53 Pages: 66-71
  • Peer Reviewed
• [Journal Article] omparison of initial growth of hypernodulation soybean mutants, NOD1-3, NOD2-4, and NOD3-7, and their parent cv (2006)
  • Author(s): Sayuri Ito, Norikuni Ohtake, Kuni Sueyoshi, Takuji Ohyama
  • Journal Title: Williams. Bull. Facul. Agric. Niigata Univ 59 Pages: 39-43
• [Journal Article] 硝酸イオンによるダイズ根粒の肥大生長と窒素固定活性の阻害機構 (2006)
  • Author(s): 伊藤小百合、大竹憲邦、末吉邦、大山卓爾
  • Journal Title: 化学と生物 44 Pages: 752-756
  • NAID: 10018329479
• [Journal Article] Allocation of photosynthetic products in hypernodulation mutant of soybean NOD1-3 in early stage of nodule formation (2006)
  • Author(s): Sayuri Ito, Norikuni Ohtake, Kuni Sueyoshi, Takuji Ohyama
  • Journal Title: Bull. Facul. Agric. Niigata Univ 59 Pages: 33-38
  • NAID: 110004748831
• [Journal Article] Allocation of photosynthetic products in soybean during early stages of nodule formation (2006)
  • Author(s): Sayuri Ito, Norikuni Ohtake, Kuni Sueyoshi, Takuji Ohyama
  • Journal Title: Soil Sci. Plant Nutr 52 Pages: 438-443
  • NAID: 110006251049
  • Peer Reviewed
• [Journal Article] Comparison of initial growth of hypernodulation soybean mutants, NOD1-3, NOD2-4, and NOD3-7, and their parent cv (2006)
  • Author(s): Sayuri Ito, Norikuni Ohtake, Kuni Sueyoshi, Takuji Ohyama
  • Journal Title: Williams. Bull. Facul. Agric. Niigata Univ 59 Pages: 39-43
• [Journal Article] Allocation of photosynthetic products in soybean during early stages of nodule formation (2006)
  • Author(s): Sayuri ITO, Norikuni OHTAKE, Kuni SUEYOSHI, Takuji OHYAMA
  • Journal Title: Soil Sci. Plant Nutr. 52 Pages: 438-443
  • NAID: 110006251049
• [Journal Article] Allocation of Photosynthetic Products in Hypernodulation Mutant of Soybean NOD1-3 in the Early Stage of Nodule Formation (2006)
  • Author(s): Sayuri ITO, Norikuni OHTAKE, Kuni SUEYOSHI, Takuji OHYAMA
  • Journal Title: Bull. Facul. Agric. Niigata Univ. 59・1 Pages: 33-38
  • NAID: 110004748831
• [Journal Article] Comparison of initial growth of hypernodulation soybean mutants, NOD1-3, NOD2-4 and NOD3-7, and their parent cv. Williams (2006)
  • Author(s): Sayuri ITO, Norikuni OHTAKE, Kuni SUEYOHSI, Takuji OHYAMA
  • Journal Title: Bull. Facul. Agric. Niigata Univ. 59・1 Pages: 38-43
  • NAID: 110004748832
• [Journal Article] 硝酸イオンによるダイズ根粒の肥大生長と窒素固定活性の阻害構造 (2006)
  • Author(s): 大山卓爾, 伊藤小百合, 大竹憲邦, 末吉邦
  • Journal Title: 化学と生物 44・11 Pages: 752-756
• [Presentation] 窒素、光合成産物によるダイズ根粒の形成と生長の制御 (2009)
  • Author(s): 加藤太一・伊藤小百合・佐々木誉臣・大竹憲邦・末吉邦・大山卓爾
  • Organizer: 植物微生物研究会第19回研究交流会
  • Place of Presentation: 松本
• [Presentation] 明暗期におけるダイズ根粒と根の生長に及ぼす硝酸の効果 (2009)
  • Author(s): 齋藤明徳・伊藤小百合・七夕高也・田嶋誠也・大竹憲邦・末吉邦・大山卓爾
  • Organizer: 植物微生物研究会第19回研究交流会
  • Place of Presentation: 松本
• [Presentation] 窒素、光合成産物によるダイズ根粒の形成と生長の制御 (2009)
  • Author(s): 加藤太一・伊藤小百合・佐々木誉臣・大竹憲邦・末吉邦・大山卓爾
  • Organizer: 植物微生物研究会第19回研究交流会
  • Place of Presentation: 長野県松本市
• [Presentation] 明暗期におけるダイズ根粒と根の生長に及ぼす硝酸の効果 (2009)
  • Author(s): 齋藤明徳・伊藤小百合・七夕高也・田嶋誠也・大竹憲邦・末吉邦・大山卓爾
  • Organizer: 植物微生物研究会第19回研究交流会
  • Place of Presentation: 長野県松本市
• [Presentation] ダイス根粒超着生変異株における根粒以外の形質の調査 (2008)
  • Author(s): 加藤太一, 伊藤小百合, 大竹憲邦, 末吉邦, 大山卓爾
  • Organizer: 日本土壌肥料学会2008年度愛知大会
  • Place of Presentation: 名古屋市立大学
  • Year and Date: 2008-09-09
• [Presentation] 硝酸によるダイス根粒の肥大生長阻害について〈光および根の伸長との関連〉 (2008)
  • Author(s): 斎藤明徳, 木村拓也, 大竹憲邦, 末吉邦, 大山卓爾
  • Organizer: 日本土壌肥料学会2008年度愛知大会
  • Place of Presentation: 名古屋市立大学
  • Year and Date: 2008-09-09
• [Presentation] ダイズ根粒超着生変異株における根粒以外の形質の調査 (2008)
  • Author(s): 加藤太一、伊藤小百合、大竹憲邦、末吉邦、大山卓爾
  • Organizer: 日本土壌肥料学会2008年度愛知大会
• [Presentation] 硝酸によるダイズ根粒の肥大生長阻害について<光および根の伸長との関連> (2008)
  • Author(s): 斎藤明徳、木村拓也、大竹憲邦、末吉邦、大山卓爾
  • Organizer: 日本土壌肥料学会2008年度愛知大会
• [Presentation] Nitrogen fixation research progress Nova Scientific Publishers Inc (2008)
  • Author(s): Takuji OHYAMA, Norikuni OHTAKE, Kuni SUEYOSHI, Kaushal TEWARI, oshihiko TAKAHASHI, Sayuri ITO, Toshikazu NISHIWAKI, Yoshifumi NAGUMO, Satomi ISHI,and Takashi SATO
• [Presentation] ダイズ葉柄から供与した植物ホルモンの移動と根粒形成に及ぼす影響 (2007)
  • Author(s): 種田祐士、伊藤小百合、大竹憲邦、末吉 邦、大山卓爾
  • Organizer: 日本土壌肥料学会2007年度東京大会
  • Place of Presentation: 東京農業大学世田谷キャンパス
  • Year and Date: 2007-08-23
• [Presentation] Comparison of leaf growth of hypernodulation soybean mutants, NOD1-3, NOD2-4, and NOD3-7 with their parent cv (2007)
  • Author(s): Sayuri Ito, Norikuni Ohtake, Kuni Sueyoshi, Takuji Ohyama
  • Organizer: Williams. 15th International Congress on Nitrogen Fixation
  • Place of Presentation: Cape Town
• [Presentation] Mechanism of quick and reversible inhibition of soybean nodule growth and nitrogen fixation activity by nitrate and its metabolites (2007)
  • Author(s): Takuji Ohyama, Akihiko Yamazaki, Natsumi Yamashita, Takuya Kimura, Sayuri Ito, Norikuni Ohtake, Kuni Sueyoshi
  • Organizer: 15th International Congress on Nitrogen Fixation
  • Place of Presentation: Cape Town
• [Presentation] ダイズ葉柄から供与した植物ホルモンの移動と根粒形成に及ぼす影響 (2007)
  • Author(s): 種田祐士、伊藤小百合、大竹憲邦、末吉邦、大山卓爾
  • Organizer: 日本土壌肥料学会2007年度東京大会
• [Book] Nitrogen fixation and metabolism of soybean (2009)
  • Author(s): Takuji OHYAMA, Norikuni OHTAKE, Kuni SUEYOSHI, Kaushal TEWARI, Yoshihiko TAKAHASHI, Sayuri ITO, Toshikazu NISHIWAKI, Yoshifumi NAGUMO, Satomi ISHII, Takashi SATO
  • Publisher: Nova Scientific Publishers Inc
• [Book] Nitrogen Fixation and Metabolism in Soybean Plants. (2009)
  • Author(s): Takuji OHYAMA, Norikuni OHTAKE, Kuni SUEYOSHI, Kaushal TEWARI, Yoshihiko TAKAHASHI, Sayuri ITO, Toshikazu NISHIWAKI, Yoshifumi NAGUMO, Satomi ISHII, Takashi SATO
  • Total Pages: 131
  • Publisher: Nova Science Publishers, Inc.(New York)
• [Book] Biological Nitrogen Fixation: Towards Poverty Alleviation through Sustainable Agriculture (2008)
  • Author(s): Takuji OHYAMA, Akihiko YAMAZAKI, Natsumi YAMASHITA, Takuya KIMURA, Sayuri ITO, Norikuni OHTAKE, Kuni SUEYOSHI
  • Publisher: Springer
• [Book] Biological Nitrogen Fixation: Towards Poverty Alleviation through Sustainable Agriculture (2008)
  • Author(s): Sayuri ITO, Norikuni OHTAKE, Kuni SUEYOSHI. Takuji OHYAMA
  • Publisher: Springer
• [Book] Nova Scientific Publishers Inc (2008)
  • Author(s): akuji OHYAMA, Norikuni OHTAKE, Kuni SUEYOSHI, Kaushal TEWARI, Yoshihiko TAKAHASHI, Sayuri ITO, Toshikazu NISHIWAKI, Yoshifumi NAGUMO, Satomi ISHII, Takashi SATO
  • Publisher: Nitrogen fixation research progress
• [Book] Nitrogen Fixation Research Progress. (2008)
  • Author(s): Takuji OHYAMA, Norikuni OHTAKE, Kuni SUEYOSHI, Kaushal TEWARI, Yoshihiko TAKAHASHI, Sayuri ITO, Toshikazu NISHIWAKI, Yoshifumi NAGUMO, Satomi ISHII, Takashi SATO
  • Total Pages: 94
  • Publisher: Nova Science Publishers, Inc.
• [Book] Biological Nitrogen Fixation : Towards Poverty Alleviation through Sustainable Agriculture. (2008)
  • Author(s): Takuji OHYAMA, Akihiko YAMAZAKI, Natsumi YAMASHITA, Takuya KIMURA, Sayuri ITO, Norikuni OHTAKE, Kuni SUEYOSHI
  • Total Pages: 6
  • Publisher: Springer
• [Remarks] 総説(1) 大山卓爾、伊藤小百合、大竹憲邦、末吉邦、化学と生物、44(11), 2006,752-756