• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification of human cardiomyogenic factor

Research Project

Project/Area Number 18390116
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionNational Research Institute for Child Health and Development

Principal Investigator

UMEZAWA Akihiro  National Research Institute for Child Health and Development, 生殖・細胞医療研究部, 部長 (70213486)

Co-Investigator(Kenkyū-buntansha) HATA Junichi  国立成育医療センター(研究所), 名誉総長 (90051614)
MAKIHO Hatsune  国立成育医療センター(研究所), 生殖細胞医療研究部, 共同研究員 (90392498)
SAI Syoko  国立成育医療センター(研究所), 生殖細胞医療研究部, 共同研究員 (80392497)
AKUTSU Hidenori  国立成育医療センター(研究所), 生殖細胞医療研究部・生殖技術研究室, 室長 (50347225)
NASU Michiyo  国立成育医療センター(研究所), 生殖細胞医療研究部, 共同研究員 (80014116)
KURODA Masahiko  東京医科大学, 病理学研究室, 准教授 (80251304)
寺井 政憲  国立成育医療センター(研究所), 生殖・細胞医療研究部, 共同研究員 (70359917)
Project Period (FY) 2006 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥18,470,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2009: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2008: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2007: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywords循環器 / 移植 / 再生医療 / 高血圧 / 液性因子 / バイオインフォマティックス / 移植・再生医療 / 循環器・高血圧
Research Abstract

The critical event in heart formation is commitment of mesodermal cells to a cardiomyogenic fate and their migration into anterolateral regions of the embryo during gastrulation. In this process, morphogenic movements and cardiac fate determination are regulated by cytokines. These secreted proteins play important roles in induction of cardiac transcription factors and differentiation of cardiomyocytes in amphibians and avians. Cardiomyogenic signals, indeed activate expression of cardiac specific transcriptional factors (Csx/Nkx2.5, Gata4, Mef2c), and these transcriptional factors activate expression of circulating hormones and cardiac specific proteins. Wnt family proteins have also been implicated in embryonic development, and cardiomyogenesis. In Drosophila, 'wingless', a homologue of vertebrate Wnt is involved in expression of 'tinman', through 'armadillo', and drives heart development. In vertebrates, however, Wnt1/3a, which activates the canonical Wnt/b-catenin signaling pathway leading to stabilization of b-catenin through inactivation of glycogen synthase kinase-3b, inhibits cardiomyocytic differentiation from cardiac mesoderm. Wnt11 promotes cardiac differentiation via the non-canonical pathway in Xenopus and murine embryonic cell lines. The secretion of Wnt inhibitors prevents Wnt3a secreted by the neural tube from inhibiting heart formation. In this study, we performed GeneChip analysis to identify multiple extracellular determinants, and evaluated the statistical significance of differential gene expression by NIA array analysis (http://lgsun.grc.nia.nih.gov/ANOVA/), a web-based tool for microarray data analysis. We found that Grem1 enhances the determined path to cardiomyogenesis, and that inhibition of the BMP signaling pathway is, at least in part, involved in initial determination of Grem1-promoted cardiomyogenesis.

Report

(6 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report   Self-evaluation Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (8 results)

All 2009 2008 2007 Other

All Journal Article (4 results) Remarks (2 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Gremlin enhances the determined path to cardiomyogenesis.2008

    • Author(s)
      Kami D, Shiojima I, Makino H, Matsumoto K, Takahashi Y, Ishii R, Naito AT, Toyoda M, Saito H, Watanabe M, Komuro I, Umezawa A
    • Journal Title

      PLoS One 3(6)

    • Related Report
      2009 Final Research Report
  • [Journal Article] IGFBP-4 is an inhibitor of canonical Wnt signalling required for cardiogenesis.2008

    • Author(s)
      Zhu W, Shiojima I, Ito Y, Li Z, Ikeda H, Yoshida M, Naito AT, Nishi J, Ueno H, Umezawa A, Minamino T, Nagai T, Kikuchi A, Asashima M, Komuro I
    • Journal Title

      Nature 454(7202)

      Pages: 345-349

    • NAID

      120007069172

    • Related Report
      2009 Final Research Report
  • [Journal Article] Gremlin enhances the determined path to cardiomyogenesis2008

    • Author(s)
      Kami D, Shiojima I, Makino H, Matsumoto K, Takahashi Y, Ishii R, Naito AT, Toyoda M, Saito H, Watanabe M Komuro I, Umezawa A
    • Journal Title

      PLoS One 3(6)

    • Related Report
      2008 Self-evaluation Report
  • [Journal Article] Single-cell-derived mesenchymal stem cells overexpressing Csx/Nkx2.5 and GATA4 undergo the stochastic cardiomyogenic fate and behave like transient amplifying cells.2007

    • Author(s)
      YamadaY, et al.
    • Journal Title

      Experimental Cell Reserch 313-4

      Pages: 698-706

    • Related Report
      2006 Annual Research Report
  • [Remarks]

    • URL

      http://www.nch.go.jp/reproduction/index.html

    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://www.nch.go.jp/reproduction/link.html

    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] 心筋細胞分化誘導促進剤及びその使用方法ピオグリタゾンを用いる2009

    • Inventor(s)
      三好俊一郎, 梅澤明弘, 小川聡
    • Industrial Property Rights Holder
      財団法人ヒューマンサイエンス振興財団
    • Filing Date
      2009-07-16
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] 心筋細胞分化誘導促進剤及びその使用方法テルミサルタン、バルサルタンを用いる2007

    • Inventor(s)
      三好俊一郎, 梅澤明弘, 小川聡
    • Industrial Property Rights Holder
      財団法人ヒューマンサイエンス振興財団
    • Filing Date
      2007-12-06
    • Related Report
      2009 Final Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi