Studies on the prevention of pneumonia by habitual voluntary exercise

• Project/Area Number: 18390183
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hygiene
• Research Institution: Kagoshima University
• Principal Investigator: TAKEUCHI Toru Kagoshima University, 大学院・医歯学総合研究科, 教授 (00188161)
• Co-Investigator(Kenkyū-buntansha): MATSUGUCHI Tetsuya 鹿児島大学, 大学院・医歯学総合研究科, 教授 (10303629) ; HORIUCHI Masahisa 鹿児島大学, 大学院・医歯学総合研究, 准教 (50264403) ; AOYAMA Kouji 鹿児島大学, 大学院・医歯学総合研究科, 講師 (70117472) ; KOMATSU Masaharu 鹿児島大学, 水産学部, 助教 (30325815)
• Research Collaborator: IZUMO Kimiko 鹿児島大学, 大学院・医歯学総合研究科, 大学院学生 ; MIYAHARA Emiko 鹿児島大学, 大学院・医歯学総合研究科, 大学院学生
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥16,700,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥2,100,000); Fiscal Year 2008: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2007: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2006: ¥7,600,000 (Direct Cost: ¥7,600,000)
• Keywords: 習慣的運動 / 自発運動 / 肺 / 肺炎 / 遺伝子発現 / 自然免疫 / 酸化ストレス / サーファクタント / 構造タンパク / 運動 / 活性酸素 / 代謝

Research Abstract

回転車を用いた長期間自発運動を行えるマウス実験システムを確立し、マウスを24週間飼育し、以下の結果を得た。
(1) マウスは1日平均5.6km走行した。
(2) 運動群は非運動群より摂食量は多かったが、内臓脂肪量は少なかった。
(3) 肺の遺伝子発現解析で、運動群は非運動群より構造タンパク並びに細胞外抗酸化酵素遺伝子の発現が高く、サーファクタント関連タンパク・細胞内抗酸化酵素・殺菌関連タンパクの遺伝子発現が低かった。
習慣的な自発運動は肺の遺伝子発現を変容させることが明らかになり、宿主の肺炎感受性や肺機能を修飾する可能性が示唆された。

Research Products

• [Journal Article] Reduction of orthodontic tooth movement by experimentally induced periodontal inflammation in mice European Journal of Oral Sciences (2009)
  • Author(s): Okamoto A, Ohnishi T, Bandow K, Kakimoto K, Chiba N, Maeda A, Fukunaga T, Miyawaki S, Matsuguchi T
  • Journal Title: 117 Pages: 238-247
  • Peer Reviewed
• [Journal Article] JNK activity is essential for Atf4 expression and late-stage osteoblast differentiation (2009)
  • Author(s): Matsuguchi T, Chiba N, Bandow K, Kakimoto K, Masuda A, Ohnishi T
  • Journal Title: J Bone Miner Res. 24(3) Pages: 398-410
  • Peer Reviewed
• [Journal Article] JNK activity is essential for Atf4 expression and late-stage osteoblast differentiation. (2009)
  • Author(s): Matsuguchi T, Chiba N, Bandow K, Kakimoto K, Masuda A, Ohnishi T.
  • Journal Title: J Bone Miner Res. 24 Pages: 398-410
  • Peer Reviewed
• [Journal Article] Oxygen induces mutation in a strict anaerobe, Prevotella melaninogenica (2008)
  • Author(s): Takumi S, Komatsu M, Aoyama K, Watanabe K, Takeuchi T
  • Journal Title: Free Radic Biol Med. 44(10) Pages: 1857-62
  • Peer Reviewed
• [Journal Article] 運動による健康保持増進 (2008)
  • Author(s): 竹内亨
  • Journal Title: 鹿児島県医師会報 Pages: 73-74
• [Journal Article] Serine/threonine kinase, Cot/Tpl2, regulates renal cell apoptosis in ischaemia/reperfusion injury (2008)
  • Author(s): Yaomura T, Tsuboi N, Urahama Y, Hobo A, Sugimoto K, Miyoshi J, Matsuguchi T, Reiji K, Matsuo S, Yuzawa Y.
  • Journal Title: Nephrology (Carlton) 13(5) Pages: 397-404
  • Peer Reviewed
• [Journal Article] Oxygen induces mutation in a strict anaerobe, Prevotella melaninogenica. (2008)
  • Author(s): Takumi S, Komatsu M, Aoyama K, Watanabe K, Takeuchi T.
  • Journal Title: Free Radic Biol Med. 44 Pages: 1857-62
  • Peer Reviewed
• [Journal Article] Serine/threonine kinase, Cot/Tpl2, regulates renal cell apoptos is in ischaemia/reperfusion injury. (2008)
  • Author(s): Yaomura T, Tsuboi N, Urahama Y, Hobo A, Sugimoto K, Miyoshi J, Matsuguchi T, Reiji K, Matsuo S, Yuzawa Y
  • Journal Title: Nephrology 13 Pages: 397-404
  • Peer Reviewed
• [Journal Article] Oxygen induces mutation in a strict anaerobe, Prevotella melaninogenica (2008)
  • Author(s): Takumi S, Komatsu M, Aoyama K, Watanabe K, Takeuchi T.
  • Journal Title: Free Radical Biology and Medicine 44 Pages: 1857-1862
  • Peer Reviewed
• [Journal Article] Expression of chemokine receptor CCR4 and its ligands (CCL17 and CCL22) in murine contact hypersensitivity (2007)
  • Author(s): Kusumoto M, Xu B, Shi M, Matsuyama T, Aoyama K, Takeuchi T
  • Journal Title: J Interferon Cytokine Res. 27(11) Pages: 901-10
  • Peer Reviewed
• [Journal Article] Involvement of reactive oxygen species in Microcystin-LR-induced cytogenotoxicity (2007)
  • Author(s): Nong Q, Komatsu M, Izumo K, Indo HP, Xu B, Aoyama K, Majima HJ, Horiuchi M, Morimoto K, Takeuchi T
  • Journal Title: Free Radic Res. 41(12) Pages: 1326-37
  • Peer Reviewed
• [Journal Article] Lack of lymphoid chemokines CCL19 and CCL21 enhances allergic airway inflammation in mice (2007)
  • Author(s): Xu B, Aoyama K, Kusumoto M, Matsuzawa A, Butcher EC, Michie SA, Matsuyama T, Takeuchi T
  • Journal Title: Int Immunol 19(6) Pages: 775-84
  • Peer Reviewed
• [Journal Article] Copper-transporting P-type ATPase, ATP7A, confers multidrug resistance and its expression is related to resistance to SN-38 in clinical colon cancer (2007)
  • Author(s): Owatari S, Akune S, Komatsu M, Ikeda R, Firth SD, Che XF, Yamamoto M, Tsujikawa K, Kitazono M, Ishizawa T, Takeuchi T
  • Journal Title: Cancer Res. 67(10) Pages: 4860-8
  • Peer Reviewed
• [Journal Article] Involvement of mitogen-activated protein kinase signaling pathways in microcystin-LR-induced apoptosis after its selective uptake mediated by OATP1B1 and OATP1B3 (2007)
  • Author(s): Komatsu M, Furukawa T, Ikeda R, Takumi S, Nong Q, Aoyama K, Akiyama S, Keppler D, Takeuchi T
  • Journal Title: Toxicol Sci. 97(2) Pages: 407-16
  • Peer Reviewed
• [Journal Article] Ceramide inhibits LPS-induced production of IL-5, IL-10, and IL-13 from mast cells. (2007)
  • Author(s): Chiba N, Masuda A, Yoshikai Y, Matsuguchi T
  • Journal Title: J Cell Physiol. 213(1) Pages: 126-36
  • Peer Reviewed
• [Journal Article] Effects of Anaerobic Exercise and Aerobic Exercise on Biomarkersof Oxidative Stress (2007)
  • Author(s): Shi M, Wang X, Yamanaka T, Ogita F, Nakatani K, Takeuchi T
  • Journal Title: Environmental Health and Preventive Medicine 12 Pages: 202-208
  • Peer Reviewed
• [Journal Article] Essential role of GATA transcriptional factors in the activation of mast cells (2007)
  • Author(s): Masuda A, Hashimoto K, Yokoi T, Doi T, Kodama T, Kume H, Ohno K, Matsuguchi T
  • Journal Title: J Immunol. 178(1) Pages: 360-8
  • Peer Reviewed
• [Journal Article] Low-intensity pulsed ultrasound (LIPUS) induces RANKL, MCP-1, and MIP-1beta expression in osteoblasts through the angiotensin II type 1 receptor (2007)
  • Author(s): Bandow K, Nishikawa Y, Ohnishi T, Kakimoto K, Soejima K, Iwabuchi S, Kuroe K, Matsuguchi T
  • Journal Title: J Cell Physiol 211(2) Pages: 392-8
  • Peer Reviewed
• [Journal Article] Effects of Anaerobic Exercise and Aerobic Exercise on Biomarkers of Oxidative Stress (2007)
  • Author(s): Shi M, Wang X, Yamanaka T, Ogita F, Nakatani K, Takeuchi T.
  • Journal Title: Environmental Health and Preventive Medicine 12 Pages: 202-208
  • Peer Reviewed
• [Journal Article] Involvement of reactive oxygen species in MCLR-induced cytogenotoxicity (2007)
  • Author(s): Nong QQ, Komatsu M, Izumo K, Indo PH, Xu BH, Aoyama K, Majima HJ, Horiuchi M, Morimoto K, Takeuchi T.
  • Journal Title: Free Radical Eesearch 41 Pages: 1326-1337
  • Peer Reviewed
• [Journal Article] Generation of a novel fluorescent product, monochlorofluorescein from dichloro- fluorescin by photo-irradiation (2006)
  • Author(s): Matsugo S, Sasai M, Shinmori H, Yasui F, Takeuchi M, Takeuchi T
  • Journal Title: Free Radic Res. 40(9) Pages: 959-65
  • Peer Reviewed
• [Journal Article] 4-Nitroquinoline 1-oxide forms 8-hydroxydeoxyguanosine in human fibroblasts through reactive oxygen species (2006)
  • Author(s): Arima Y, Nishigori C, Takeuchi T, Oka S, Morimoto K, Utani A, Miyachi Y
  • Journal Title: Toxicol Sci. 91(2) Pages: 382-92
  • Peer Reviewed
• [Journal Article] Mature hepatocyte growth factor/scatter factor on the surface of human granulocytes is released by a mechanism involving activated factor Xa (2006)
  • Author(s): Ohnishi T, Kakimoto K, Bandow K, Lowenstein CJ, Daikuhara Y, Matsuguchi T
  • Journal Title: J Immunol. 176(11) Pages: 6945-53
  • Peer Reviewed
• [Journal Article] 4-Nitroquinoline 1-oxide forms 8-hydroxydeoxyguanosine in human fibroblasts through reactive oxygen species. (2006)
  • Author(s): Arima Y., Nishigori C., Takeuchi T., Oka S., Morimoto K., Utani A., Miyachi Y.
  • Journal Title: Toxicological Sciences 91・2 Pages: 382-392
• [Journal Article] Generation of a novel fluorescent product, monochlorofluorescein from dichlorofluorescin by photo-irradiation. (2006)
  • Author(s): Matsugo S., Sasai M., Shinmori H., Yasui F., Takeuchi M., Takeuchi T.
  • Journal Title: Free Radical Research 40・9 Pages: 959-965
• [Journal Article] Contact sensitizer potassium dichromate alters lymphocyte populations in draining lymph nodes and blood in mice.
  • Author(s): Aoyama K., Xu BH., Cook R.E., Kusumoto M., Shi M., Michie S.A., Takeuchi T.
  • Journal Title: Toxicology Mechanisms and Methods (in press)
• [Journal Article] Involvement of mitogen-activated protein kinase signaling pathways in microcystin-LR-induced apoptosis after its selective uptake mediated by OATP1B1 and OATP1B3.
  • Author(s): Komatsu M., Furukawa T., Ikeda R., Takumi S., Nong Q., Aoyama K., Akiyama S., Keppler D., Takeuchi T.
  • Journal Title: Toxicological Sciences (in press)
• [Journal Article] Lack of lymphoid chemokines CCL19 and CCL21 enhances allergic airway inflammation in mice.
  • Author(s): Xu BH, Aoyama K, Kusumoto M, Matsuzawa A, Butcher EC, Michie SA, Matsuyama T, Takeuchi T.
  • Journal Title: International Immunology (in press)
• [Journal Article] Copper-transporting P-type ATPase, ATP7A, Confers Multidrug Resistance and its Expression is Related to Resistance to SN-38 in Clinical Colon Cancer.
  • Author(s): Owatari S., Akune S, Komatsu M, Ikeda R, Firth SD, Che XF, Yamamoto M, Tsujikawa K, Kitazono M, Ishizawa T, Takeuchi T, Aikou T, Mercer JFB, Akiyama S, Furukawa T.
  • Journal Title: Cancer Research (in press)
• [Presentation] 自発運動が肺がん発症に与える影響 : 化学発がんモデルマウスを用いた検討 (2009)
  • Author(s): 出雲公子、堀内正久、川口博明、竹下温子、青山公治、竹内亨
  • Organizer: 第79回日本衛生学会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-30
• [Presentation] 長期の習慣的な運動が血中アミノ酸に与える影響 (2009)
  • Author(s): 竹下温子、堀内正久、出雲公子、青山公治、竹内亨
  • Organizer: 第79回日本衛生学会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-30
• [Presentation] 変異原物質による薬剤耐性菌の誘発に関する研究 (2009)
  • Author(s): 宮原恵祢子,内匠正太,宮之原弘晃,西順一郎,吉家清貴,小田紘,小松正治,青山公治,堀内正久,竹内亨
  • Organizer: 第79回日本衛生学会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-30
• [Presentation] 変異原物質による薬剤耐性菌の誘発に関する研究 (2009)
  • Author(s): 宮原恵祢子, 内匠正太, 宮之原弘晃, 西順一郎, 吉家清貴, 小田紘, 小松正治, 青山公治, 堀内正久, 竹内亨
  • Organizer: 日本衛生学会
  • Place of Presentation: 東京(北里大学)
  • Year and Date: 2009-03-30
• [Presentation] 長期の習慣的な運動が血中アミノ酸に与える影響 (2009)
  • Author(s): 竹下温子, 堀内正久, 出雲公子, 青山公治, 竹内亨
  • Organizer: 日本衛生学会
  • Place of Presentation: 東京(北里大学)
  • Year and Date: 2009-03-30
• [Presentation] 自発運動が肺がん発症に与える影響 : 化学発がんモデルマウスを用いた検討 (2009)
  • Author(s): 出雲公子, 堀内正久, 川口博明, 竹下温子, 青山公治, 竹内亨
  • Organizer: 日本衛生学会
  • Place of Presentation: 東京(北里大学)
  • Year and Date: 2009-03-30
• [Presentation] 習慣的運動が発がんに影響を与えるか? : 回転車付きケージと肺がん易発症マウスを用いた評価モデルの確立 第2報 (2008)
  • Author(s): 堀内正久、出雲公子、川口博明、青山公治、竹内亨
  • Organizer: 第6回日本予防医学会
  • Place of Presentation: 東京
  • Year and Date: 2008-11-29
• [Presentation] 習慣的運動が発がんに影響を与えるか? : 回転車付きケージと肺がん易発症マウスを用いた評価モデルの確立 第2報 (2008)
  • Author(s): 堀内正久, 出雲公子, 川口博明, 青山公治, 竹内亨
  • Organizer: 日本予防医学会
  • Place of Presentation: 東京(東京医科大学)
  • Year and Date: 2008-11-29
• [Presentation] 習慣的運動による遺伝子発現の変動 (2008)
  • Author(s): 米澤千花、出雲公子、坂東健二郎、小松正治、青山公治、堀内正久、松口徹也、竹内亨
  • Organizer: 第78回日本衛生学会
  • Place of Presentation: 熊本
  • Year and Date: 2008-03-31
• [Presentation] 習慣的運動による遺伝子発現の変動 (2008)
  • Author(s): 米澤千花、出雲公子、坂東健二郎、小松正治、青山公治、堀内正久、松口徹也、竹内亨
  • Organizer: 第78回日本衛生学会
  • Place of Presentation: 熊本市
  • Year and Date: 2008-03-31
• [Presentation] Environmental mutagens as a possible cause for the emergence of drug resistant bacteria (2007)
  • Author(s): Nishie M, Takumi S, Miyanohara H, Nishi J, Yoshiie K, Oda h, Komatsu M, Aoyama K, Horiuchi M, Kishi F, Takeuchi M, Takeuchi T
  • Organizer: 1st Asian Conference on Environmental Mutagens/36^<th> Annual Meeting of the Japanese Environmental Mutagen Society
  • Place of Presentation: 北九州
  • Year and Date: 2007-11-29
• [Presentation] 習慣的運動が癌の発症に影響を与えるか- : 回転車付きケージと肺癌易発症マウスを用いた評価、モデルの確立 (2007)
  • Author(s): 堀内正久、出雲公子、川口博明、小松正治、青山公治、竹内亨
  • Organizer: 第5回日本予防医学会
  • Place of Presentation: 鹿児島
  • Year and Date: 2007-11-24
• [Presentation] 抗老化サプリメント、Dehydroepi-androsterone(DHEA)の安全性に関する研究 (2007)
  • Author(s): 出雲公子、小松正治、青山公治、坂東健二郎、堀内正久、松口徹也、竹内亨
  • Organizer: 第77回日本衛生学会
  • Place of Presentation: 大阪
  • Year and Date: 2007-03-26
• [Book] 森林と運動療法, 森林医学, (森本・宮崎・平野編)(東京) (2006)
  • Author(s): 青山公治, 竹内亨, 古野純典
  • Publisher: 朝倉書店
• [Book] 環境保健, 産業衛生, New予防医学・公衆衛生学 改訂第2版(岸・古野・小泉・大前編) (2006)
  • Author(s): 竹内亨
  • Publisher: 南江堂
• [Book] 酸素高感受性生体システム(嫌気性菌)における酸化ストレス(分担), 酸化ストレス-フリーラジカル医学生物学の最前線 ver.2, 別冊医学のあゆみ, (吉川編) (2006)
  • Author(s): 竹内亨
  • Publisher: 医歯薬出版
• [Book] 酸素高感受性生体システム(嫌気性菌)における酸化ストレス(分担),吉川編 : 酸化ストレス-フリーラジカル医学生物学の最前線 ver.2,59-62, 別冊医学のあゆみ, (2006)
  • Author(s): 竹内享(分担執筆)
  • Total Pages: 450
  • Publisher: 医歯薬出版
• [Remarks] 研究成果一部を以下のサイトに掲載した。
  • URL: http://www.kufm.kagoshima-u.ac.jp/%7Eeisei001/Research%20project.htm
• [Remarks]
  • URL: http://www.kufm.kagoshima-u.ac.jp/%7Eeisei001/Research%20project.htm