Analysis of ion channels associated with auditory sensation and construction of a model for hereditary hearing impairment

• Project/Area Number: 18390460
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Nagoya City University
• Principal Investigator: SHIMADA Shoichji (SHIMADA Shoichi) Nagoya City University, 大学院・医学系研究科, 教授 (20216063)
• Co-Investigator(Kenkyū-buntansha): MURAKAMI Shingo 名古屋市立大学, 大学院・医学研究科, 教授 (80157750) ; UGAWA Shinya 名古屋市立大学, 大学院・医学研究科, 教授 (20326135) ; ISHIDA Yusuke 大阪大学, 大学院・医学系研究科, 講師 (30381809)
• Project Period (FY): 2006 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥17,000,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥3,000,000); Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2008: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2007: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2006: ¥4,000,000 (Direct Cost: ¥4,000,000)
• Keywords: 聴覚 / 内耳 / 有毛細胞 / イオンチャネル / ノックアウトマウス

Research Abstract

We examined whether amiloride-sensitive proton-gated cation channels ASICs (acid-sensing ion channels) are expressed in the stereocilia of mouse cochlear hair cells. In situ hybridization studies revealed that ASIC1b was expressed in the inner and outer hair cells. Fluorescent immunohistochemistry and immunogold electron microscopic studies demonstrated that ASIC1b was located at the insertions of stereocilia into the hair cells. Our findings provide a novel molecular key to the understanding of cochkear physiology and pathophysiology.

Research Products

• [Journal Article] P2X(2)- and P2X(3)-positive fibers in fungiform papillae originate from the chorda tympani but not the trigeminal nerve in rats and mice. (2009)
  • Author(s): Ishida Y, Ugawa S, Ueda T, Yamada T, Shibata Y, Hondoh A, Inoue K, Yu Y, Shimada S.
  • Journal Title: J Comp Neurol. 514 Pages: 131-144
  • Peer Reviewed
• [Journal Article] P2X(2)- and P2X(3)-positive fibers in fungiform papillae originate from the chorda tympani but not the trigeminal nerve in rats and mice. (2009)
  • Author(s): Ishida Y, et al.
  • Journal Title: J Comp Neurol Volume: 514 Pages: 131-144
  • Peer Reviewed
• [Journal Article] TRPV3, a thermosensitive channel is expressed in mouse distal colon epithelium. (2009)
  • Author(s): Ueda T, et al.
  • Journal Title: Biochem Biophys Res Commun Volume: 383 Pages: 130-134
  • Peer Reviewed
• [Journal Article] Upregulation of the P2X7 receptor after cryogenic injury to rat brain. (2009)
  • Author(s): Yu Y, et al.
  • Journal Title: Neuroreport Volume: 20 Pages: 393-397
  • Peer Reviewed
• [Journal Article] The CaV3.1 T-type Ca2+channel contributes to voltage-dependent calcium currents in rat outer hair cells. (2008)
  • Author(s): Inagaki A, Ugawa S, Yamamura H, Murakami S, Shimada S.
  • Journal Title: Brain Res. 1201 Pages: 68-77
  • Peer Reviewed
• [Journal Article] Hypotonic stimuli enhance proton-gated currents of acid-sensing ion channel-lb. (2008)
  • Author(s): Ugawa S, Ishida Y, Ueda T, Yu Y, Shimada S.
  • Journal Title: Biochem Biophys Res Commun. 367 Pages: 530-534
  • Peer Reviewed
• [Journal Article] Hypotonic stimuli enhance protein-gated currents of acid-sensing ion hannel-lb (2008)
  • Author(s): Ugawa S, Ishida Y, Ueda T, Yu Y, Shimada S
  • Journal Title: Biochem Biophys Res Commun 367 Pages: 530-534
  • Peer Reviewed
• [Journal Article] The CaV3.1 T-type Ca2+channel contributes to voltage-dependent calcium currents in rat outer hair cells (2008)
  • Author(s): Inagaki A, Ugawa S, Yamamura H, Murakami S, Shimada S
  • Journal Title: Brain Res 1201 Pages: 68-77
  • Peer Reviewed
• [Journal Article] Hypotonic stimuli enhance proton-gated currents of acid-sensing ion channel-lb. (2008)
  • Author(s): Ugawa S, et al.
  • Journal Title: Biochem Biophys Res Commun 367 Pages: 530-534
  • Peer Reviewed
• [Journal Article] The CaV3.1 T-type Ca2+channel contributes to voltage-dependent calcium currents in rat outer hair cells. (2008)
  • Author(s): Inagaki A, et al
  • Journal Title: Brain Res 1201 Pages: 68-77
  • Peer Reviewed
• [Journal Article] Cellular localization of P2X7 receptor mRNA in the rat brain. (2008)
  • Author(s): Yu Y, et al.
  • Journal Title: Brain Res 1194 Pages: 45-55
  • Peer Reviewed
• [Journal Article] Nafamostat mesilate reversibly blocks acid-sensing ion channel currents. (2007)
  • Author(s): Ugawa S, Ishida Y, Ueda T, Inoue K, Nagao M, Shimada S.
  • Journal Title: Biochem Biophys Res Commun. 363 Pages: 203-208
  • Peer Reviewed
• [Journal Article] Nafamostat mesilate reversibly blocks acid-sensing ion channel currents (2007)
  • Author(s): Ugawa S, Ishida Y, Ueda T, Inoue K, Nagao M, Shimada S
  • Journal Title: Biochem Biophys Res Commun 363 Pages: 203-208
  • Peer Reviewed
• [Journal Article] Nafamostat mesilate reversibly blocks acid-sensing ion channel currents. (2007)
  • Author(s): Ugawa S, et. al.
  • Journal Title: Biochem Biophys Res Commun 363 Pages: 203-208
  • Peer Reviewed
• [Journal Article] Acid-sensing ion channel-lb in the stereocilia of mammalian cochlear hair cells. (2006)
  • Author(s): Ugawa S, Inagaki A, Yamamura H, Ueda T, Ishida Y, Kajita K, Shimizu H, Shimada S.
  • Journal Title: Neuroreport. 17 Pages: 1235-1239
  • Peer Reviewed
• [Journal Article] Acid sensing ion channel-lb in the stereocilia of mammalian cochlear hair cells (2006)
  • Author(s): Ugawa S, Inagaki A, Yamamura H, Ueda T, Ishida Y, Kajita K, Shimizu H, Shimada S
  • Journal Title: Neuroreport 17 Pages: 1235-1239
  • Peer Reviewed
• [Journal Article] Acid-sensing ion channel-1b in the stereocilia of mammalian cochlear hair cells. (2006)
  • Author(s): Ugawa S., et al.
  • Journal Title: Neuroreport 17 Pages: 1235-1239
  • NAID: 110007409647
• [Journal Article] A novel spliced variant of the epithelial Na+ channel delta-subunit in the human brain. (2006)
  • Author(s): Yamamura H., et al.
  • Journal Title: Biochem Biophys Res Commun 349 Pages: 317-321
• [Presentation] 内耳蝸牛有毛細胞に発現する酸感受性イオンチャネルASIClbの生理的役割 (2010)
  • Author(s): 鵜川眞也、植田高史
  • Organizer: 日本解剖学会第115回全国学術集会
  • Place of Presentation: 岩手
  • Year and Date: 2010-03-28
• [Presentation] 低浸透圧刺激は酸感受性イオンチャンネル-1bのプロトン開口電流を増強する (2008)
  • Author(s): 鵜川眞也、石田雄介、植田高史、島田昌一
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-09
• [Presentation] マウス蝸牛有毛細胞における酸感受性イオンチャネル(ASIC)1bの発現 (2007)
  • Author(s): 鵜川眞也、石田雄介、植田高史、島田昌一
  • Organizer: 第50回日本神経化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-10
• [Presentation] Hair cell-ASIC is a candidate for the mechano-electric transduction channel of mammalian hair cells. (2006)
  • Author(s): 鵜川眞也、石田雄介、島田昌一
  • Organizer: 日本生物学的精神医学会第28回・日本神経精神薬理学会第36回・日本神経化学第49回大会合同年会
  • Place of Presentation: 名古屋
  • Year and Date: 2006-09-14