Studies on speciation of black flies and filaria transmission in Southeast Asia

• Project/Area Number: 18406011
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Oita University
• Principal Investigator: TAKAOKA Hiroyuki Oita University, 医学部, 教授 (00094152)
• Co-Investigator(Kenkyū-buntansha): OTSUKA Yasushi 大分大学, 医学部, 助教 (00244161) ; FUKUDA Masako 大分大学, 総合科学研究支援センター, 助教 (00156788)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥5,270,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥870,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: 感染症 / 昆虫 / 進化 / 寄生虫 / 生物多様性 / ブユ / タイ / フィリピン / マレーシア:フィリピン / 国際研究者交流 / タイ:フィリピン

Research Abstract

東南アジアにおける吸血性昆虫ブユ(現在300種超, ブユ属の13亜属に分類)の1亜属および他の2亜属の7種グループの構成種(計75種)について, ミトコンドリア16S rRNA遺伝子の塩基配列を決め, 系統樹を作成し, グループ別に地理的分布も考慮して種分化の推定を行った. その結果, 多くのグループにおいて, 狭い地域内における種分化が示唆された. また, タイ北部において, 3種のブユが, 各々, 異なる3種のフィラリアを媒介していることを明らかにした.

Research Products

• [Journal Article] Simulium(Gomphostilbia)hoiseni sp. nov. (Diptera : Simuliidae) : a new species from Peninsular Malaysia (2008)
  • Author(s): Takaoka, H.
  • Journal Title: Med. Entomol. Zool 59 Pages: 9-14
  • Peer Reviewed
• [Journal Article] Taxonomic revision of tuberosum species-group of Simulium(Simulium)in Sabah and Sarawak, Malaysia(Diptera : Simuliidae) (2008)
  • Author(s): Takaoka, H.
  • Journal Title: Med. Entomol. Zool 59 Pages: 55-80
  • NAID: 110006812765
  • Peer Reviewed
• [Journal Article] Seasonal and diurnal biting activities and zoonotic filarial infections of two Simulium species(Diptera : Simuliidae)in northern Thailand (2008)
  • Author(s): Ishii, Y., Choochote, W., Bain, O., Fukuda, M., Otsuka, Y., Takaoka, H.
  • Journal Title: Parasite 15 Pages: 121-129
  • Peer Reviewed
• [Journal Article] A new species of Simulium (Gomphostilbia) (Diptera : Simuliidae)from Palawan Island, the Philippines. (2008)
  • Author(s): Takaoka, H., Tenedero, V. F.
  • Journal Title: Med. Entomol. Zool. 59 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Simulium (Gomphostilbia)hoiseni sp. nov. (Diptera : Simuliidae) : a new species from Peninsular Malaysia. (2008)
  • Author(s): Takaoka, H.
  • Journal Title: Med. Entomol. Zool. 59 Pages: 9-14
  • Peer Reviewed
• [Journal Article] Taxonomic revision of tuberosum species-group of Simulium(Simulium)in Sabah and Sarawak, Malaysia (Diptera : Simuliidae). (2008)
  • Author(s): Takaoka, H.
  • Journal Title: Med. Entomol. Zool. 59 Pages: 55-80
  • NAID: 110006812765
  • Peer Reviewed
• [Journal Article] Four new species of Simulium (Gomphostilbia)(Diptera : Simuliidae)from Sarawak, Malaysia. (2008)
  • Author(s): Takaoka, H.
  • Journal Title: Med. Entomol. Zool. 59 Pages: 181-211
  • NAID: 110007004893
  • Peer Reviewed
• [Journal Article] Eleven new species and one new record of black flies (Diptera : Simuliidae)from Bhutan. (2008)
  • Author(s): Takaoka, H., Somboon, P.
  • Journal Title: Med. Entomol. Zool. 59 Pages: 213-262
  • NAID: 110007004894
  • Peer Reviewed
• [Journal Article] Simulium(Gomphostilbia)rarum sp. nov., a new species of black fly (Diptera : Simuliidae) from Palawan Island, the Philippines. (2008)
  • Author(s): Takaoka, H., Tenedero, V. F.
  • Journal Title: Med. Entomol. Zool. 59 Pages: 319-324
  • NAID: 110007081446
  • Peer Reviewed
• [Journal Article] New species and record of black flies (Diptera : Simuliidae)from Samar, the Philippines. (2008)
  • Author(s): Takaoka, H., Tenedero, V. F.
  • Journal Title: Med. Entomol. Zool. 59 Pages: 325-336
  • NAID: 110007081444
  • Peer Reviewed
• [Journal Article] Infective larvae of five Onchocerca species from experimentally infected Simulium species in an area of zoonotic onchocerciasis in Japan (2008)
  • Author(s): Fukuda, M., Takaoka, H., Uni, S., Bain, O.
  • Journal Title: Parasite 15 Pages: 111-119
  • Peer Reviewed
• [Journal Article] Seasonal and diurnal biting activities and zoonotic filarial infections of two Simulium species (Diptera : Simuliidae)in northern Thailand (2008)
  • Author(s): Ishii. Y., Choochote, W. Bain, O., Fukuda, M., Otsuka, Y., Takaoka, H.
  • Journal Title: Parasite 15 Pages: 121-129
  • Peer Reviewed
• [Journal Article] A new species of Simulium (Gomphostilbia)(Diptera : Simuliidae)from Taiwan. (2008)
  • Author(s): Huang, Y-T., Takaoka, H.
  • Journal Title: Med. Entomol. Zool. 59 Pages: 171-179
  • NAID: 110007004892
  • Peer Reviewed
• [Journal Article] Description of the female of Simulium(Simulium) puliense (Diptera : Simuliidae)from Taiwan. (2008)
  • Author(s): Huang, Y-T., Takaoka, H.
  • Journal Title: Med. Entomol. Zool. 59 Pages: 305-308
  • NAID: 110007081450
  • Peer Reviewed
• [Journal Article] Simulium(Simulium)pingtungense, a new species of black fly (Diptera : Simuliidae)from Taiwan. (2008)
  • Author(s): Huang, Y-T., Takaoka, H.
  • Journal Title: Med. Entomol. Zool. 59 Pages: 309-317
  • NAID: 110007081448
  • Peer Reviewed
• [Journal Article] A new species of Simulium(Simulium)from Luzon Island, the Philippines(Diptera : Simuliidae) (2007)
  • Author(s): Takaoka, H.
  • Journal Title: Med. Entomol. Zool 58 Pages: 11-17
  • NAID: 110006274043
  • Peer Reviewed
• [Journal Article] Phylogenetic analysis of three subgenera : Asiosimulium, Daviesellum and Wallacellum, of the genus Simulium s. l. endemic in the Oriental Region (2007)
  • Author(s): Otsuka, Y., Aoki, C., Choochote, W., De las Llagas, L., Takaoka, H.
  • Journal Title: Med. Entomol. Zool 58 Pages: 329-333
  • NAID: 110006571286
  • Peer Reviewed
• [Journal Article] A new species of Simulium (Simulium) from Luzon Island, the Philippines (Diptera: Simuliidae). (2007)
  • Author(s): Takaoka, H.
  • Journal Title: Medical Entomology and Zoology 58 Pages: 11-17
  • NAID: 110006274043
  • Peer Reviewed
• [Journal Article] Two new and four newly recorded species of Simulium (Diptera: Simuliidae) from Mindoro Island, the Philippines. (2007)
  • Author(s): Takaoka, H.
  • Journal Title: Medical Entomology and Zoology 58 Pages: 29-43
  • NAID: 110006274045
  • Peer Reviewed
• [Journal Article] A new species of the multistriatum species-group of Simulium (Simulium) (Diptera: Simuliidae) from Northern Thailand. (2007)
  • Author(s): Takaoka, H.
  • Journal Title: Tropical Medicine and Health 35 Pages: 19-22
  • NAID: 10019492579
  • Peer Reviewed
• [Journal Article] A new species of Simulium (Simulium) from Negros Island, the Philippines (Diptera: Simuliidae). (2007)
  • Author(s): Takaoka, H.
  • Journal Title: Medical Entomology and Zoology 58 Pages: 89-97
  • NAID: 110006350436
  • Peer Reviewed
• [Journal Article] Two new and one newly recorded species of Simulium (Diptera: Simuliidae) from Palawan Island, the Philippines. (2007)
  • Author(s): Takaoka, H.
  • Journal Title: Medical Entomology and Zoology 58 Pages: 207-228
  • NAID: 110006436719
  • Peer Reviewed
• [Journal Article] Two new species of Simulium (Simulium) from Sabah, Malaysia (Diptera: Simuliidae). (2007)
  • Author(s): Takaoka, H.
  • Journal Title: Medical Entomology and Zoology 58 Pages: 291-301
  • NAID: 110006571282
  • Peer Reviewed
• [Journal Article] Phylogenetic analysis of three subgenera: Asiosimulium, Daviesellum and Wallacellum, 0f the genus Simulium s.l. endemic in the Oriental Region. (2007)
  • Author(s): Otsuka, Y.
  • Journal Title: Medical Entomology and Zoology 58 Pages: 329-333
  • Peer Reviewed
• [Journal Article] Choochote, W. A new species of Simulium(Nevermannia)from northern Thailand(Diptera : Simuliidae) (2006)
  • Author(s): Takaoka, H.
  • Journal Title: Med. Entomol. Zool 57 Pages: 83-92
  • Peer Reviewed
• [Journal Article] Four new species of Simulium(Simulium)from Luzon Island, Philippines(Diptera : Simuliidae) (2006)
  • Author(s): Takaoka, H.
  • Journal Title: Med. Entomol. Zool 57 Pages: 287-307
  • NAID: 110006197521
  • Peer Reviewed
• [Journal Article] A new species of Simulium (Nevermannia) from northern Thailand (Diptera : Simuliidae). (2006)
  • Author(s): Takaoka, H.
  • Journal Title: Medical Entomology and Zoology 57(2) Pages: 83-92
  • NAID: 110004799587
• [Journal Article] A new species of Simulium (Simulium) (Diptera : Simuliidae) from Luzon Island, Philippines. (2006)
  • Author(s): Takaoka, H.
  • Journal Title: Medical Entomology and Zoology 57(2) Pages: 99-103
  • NAID: 110004799589
• [Journal Article] A new species of the griseifrons species-group of Simulium (Simulium) (Diptera : Simuliidae) in northern Thailand. (2006)
  • Author(s): Takaoka, H.
  • Journal Title: Medical Entomology and Zoology 57(2) Pages: 115-124
  • NAID: 110004799592
• [Journal Article] A new species of Simulium (Gomphostilbia) (Diptera : Simuliidae) from northern Thailand. (2006)
  • Author(s): Takaoka, H.
  • Journal Title: Medical Entomology and Zoology 57(3) Pages: 229-233
  • NAID: 110004793088
• [Journal Article] Four new species of Simulium (Simulium) from Luzon Island, Philippines (Diptera : Simuliidae). (2006)
  • Author(s): Takaoka, H.
  • Journal Title: Medical Entomology and Zoology 57(4) Pages: 287-307
  • NAID: 110006197521
• [Journal Article] Revised description of Simulium (Simulium) forcipatum Delfinado, and description of three new related species from Luzon Island, Philippines (Diptera : Simuliidae). (2006)
  • Author(s): Takaoka, H.
  • Journal Title: Medical Entomology and Zoology 57(4) Pages: 309-326
  • NAID: 110006197522
• [Journal Article] Three new species of Simulium (Wallacellum) from Luzon Island, Philippines (Diptera : Simuliidae). (2006)
  • Author(s): Takaoka, H.
  • Journal Title: Medical Entomology and Zoology 57(4) Pages: 327-346
  • NAID: 110006197523
• [Presentation] フィリピン国サマール島で採集されたブユ属の新種および新記録種について (2008)
  • Author(s): 高岡宏行, 大塚靖
  • Organizer: 第61回日本寄生虫学会南日本支部大会・第58回日本衛生動物学会南日本支部大会・合同大会
  • Place of Presentation: 沖縄県那覇市
  • Year and Date: 2008-11-01
• [Presentation] マレーシア国サバ州およびサラワク州におけるSimulium属Simulium 亜属 tuberosum種グループに含まれる種の分類学的検討 (2008)
  • Author(s): 高岡宏行, 青木千春
  • Organizer: 第60回日本衛生動物学会大会
  • Place of Presentation: 栃木県下野市
  • Year and Date: 2008-04-19
• [Presentation] ヒトスジシマカのタイ集団のミトコンドリア遺伝子配列の多型について (2008)
  • Author(s): 大塚靖, 高岡宏行
  • Organizer: 第60回日本衛生動物学会大会
  • Place of Presentation: 栃木県下野市
  • Year and Date: 2008-04-19
• [Presentation] マレーシア国サバ州およびサラワク州におけるSimulium属Simulium亜属tuberosum種グループに含まれる種の分類学的検討 (2008)
  • Author(s): 高岡宏行, 青木千春
  • Organizer: 第60回日本衛生動物学会大会
  • Place of Presentation: 栃木県下野市
  • Year and Date: 2008-04-18
• [Presentation] タイ北部における人吸血性ブユ2種Simulium nodosum及びS. asakoae雌成虫のフィラリア自然感染率 (2007)
  • Author(s): 高岡 宏行
  • Organizer: 第60回日本寄生虫学会・第57回日本衛生動物学会南日本支部合同大会
  • Place of Presentation: 熊本市
  • Year and Date: 2007-10-27
• [Presentation] タイ北部における人吸血性ブユ2種Simulium nodosum及びS. asakoae雌成虫のフィラリア自然感染率 (2007)
  • Author(s): 石井美光, Wej Choochote, 福田昌子, 高岡宏行
  • Organizer: 第60回日本寄生虫学会・第57回日本衛生動物学会南日本大会・合同大会
  • Place of Presentation: 熊本県熊本市
  • Year and Date: 2007-10-17
• [Presentation] フィリピンにおいて見い出されたブユ属ウォレスブユ亜属の3新種について (2006)
  • Author(s): 高岡宏行, 大塚靖, 福田昌子, 青木千春
  • Organizer: 第59回日本寄生虫学会南日本支部大会・第56回日本衛生動物学会南日本支部大会・合同大会
  • Place of Presentation: 福岡県福岡市
  • Year and Date: 2006-10-28