A Genetic Engineering Approach to the Development of Novel Anti-microbial Peptide Drugs from Human Lysozyme

• Project/Area Number: 18580124
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: Kagoshima University
• Principal Investigator: IBRAHIM Hisham Radwan Kagoshima University, 農学部, 准教授 (90274836)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,920,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2006: ¥2,100,000 (Direct Cost: ¥2,100,000)
• Keywords: 医薬品剤開発 / ヒトリゾチーム / 抗菌ペプチド / 酵母発現 / 抗感染機能 / 蛋白質工学 / 機能性食品 / タンパク構造と機能 / 機能性食品ペプチド

Research Abstract

生体防御という観点からは、ヒトリゾチームの抗菌性ペプチドの発現ができれば、生体防御の強化が可能な食品や、消化器系などの傷害の治療に有効な医薬品の開発が可能になるものと考えられ、非常に有用である。本研究により、ヒトリゾチームに新規なペプチドが含まれることが明らかにした。Pichiaにより産生させることができて、これらの新規抗菌ペプチドを摂取したり投与したりすることによって、潜在的な微生物病原体に起因する感染症又は消化器系炎症等の予防や治療を行うことが期待される。

Research Products

• [Journal Article] riclosan-Lysozyme Complex : A Promising Anti-microbial Macromolecule Stable against Photooxidative Damage (2009)
  • Author(s): Md. Imranul Hoq; T Aoki; Ibrahim H.R.
  • Journal Title: Food Res. Intl. 42 Pages: 298-306
  • Peer Reviewed
• [Journal Article] Improvement of Foaming property of Egg White Protein by Phosphorylation through Dry-heating in the Presence of Pyrophosphate (2009)
  • Author(s): Hayashi, Y., Nagano, S., Enomoto, H., Li, C.P., Sugimoto, Y., H., Ibrahim H.R., Hatta, H., Takeda, C., Aoki, T.
  • Journal Title: Food Chemistry 74(1)
  • Peer Reviewed
• [Journal Article] Triclosan-Lysozyme Complex : A Promising Anti-microbial Macromolecule Stable against Photooxidative Damage (2009)
  • Author(s): Hoq, M. I., Aoki, T., Ibrahim, H. R
  • Journal Title: Food Research International 42 Pages: 298-306
  • Peer Reviewed
• [Journal Article] Triclosan-lysozymecomplex as novel antimicrobial as phenolic drug-targeting molecule (2008)
  • Author(s): Hoq, M.I.; Mitsuno, K.; Tsujino, Y.; Aoki, T.;Ibrahim, H.R.
  • Journal Title: Intl. J. Biol. Macromol. 42(5) Pages: 468-477
  • Peer Reviewed
• [Journal Article] Improvement of Functional properties of Ovotransferrin by Phosphorylation through Dry-heating in the Presence of Pyrophosphate (2008)
  • Author(s): Hayashi, Y., Li, C.P., Enomoto, H., IbrahimH.R., Sugimoto, Y., Aoki, T.
  • Journal Title: Asian-Australian Journal of Animal Science 21(4) Pages: 596-602
  • Peer Reviewed
• [Journal Article] A New Potential for Anti-Infection Therapy (2008)
  • Author(s): Ibrahim, H.R.
  • Journal Title: INFORM 19(3) Pages: 189-192
  • Peer Reviewed
• [Journal Article] 卵白オボトランスフェリンの機能性 (2008)
  • Author(s): イブラヒムヒッシャム
  • Journal Title: FOODStyle21 21(9) Pages: 34-36
• [Journal Article] A New Potential for Anti-Infection Therapy by Using Lysozyme (2008)
  • Author(s): Ibrahim, H. R.
  • Journal Title: INFORM 19 Pages: 189-192
  • Peer Reviewed
• [Journal Article] Triclosan-lysozyme complex as novel antimicrobial macromolecule : a new potential of lysozyme as phenolic drug-targeting molecule (2008)
  • Author(s): Hoq, M. I., Mitsuno, K., Tsujino, Y., Aoki, T., Ibrahim. H. R
  • Journal Title: Intl. J. Biol. Macromol. 42 Pages: 468-477
  • Peer Reviewed
• [Journal Article] 卵白オボトランスフェリンの機能性 (2008)
  • Author(s): イブラヒム ヒツシャム
  • Journal Title: FOODStyle21 12 Pages: 34-36
• [Journal Article] Triclosan-lysozyme complex as novel antimicrobial macromolecule: a new potential of lysozyme as phenolic drug-targeting molecule (2008)
  • Author(s): Hoq, M.I; Mitsuno, K.; Tsujino, Y.; Aski, T.; Ibrahim, H.R.
  • Journal Title: Intl. J. Biol. Macromol. (Accepted)10-MAR-2008(発行中)
  • Peer Reviewed
• [Journal Article] Ovotransferrin Possesses SOD-like Superoxide Anion Scavenging Activity that is Promoted by Copper and Manganese Binding (2007)
  • Author(s): Ibrahim, H.R.; Hoq, M.I.; Aoki, T.
  • Journal Title: Intl. J. Biol. Macromol. 41(5) Pages: 631-640
  • Peer Reviewed
• [Journal Article] Antimicrobial peptides derived from hen egg lysozyme with inhibitory effect against Bacillus species (2007)
  • Author(s): Abdou, A.M., Higashiguchi, S., Aboueleinin, A.M., Kim, M. and Ibrahim, H.R.
  • Journal Title: Food Control 18 Pages: 173-178
  • Peer Reviewed
• [Journal Article] Effect Of Saccharomyces cerevisiae on Cell Wall Constituents Digestion in Sheep Fed Berseem(Trifolium alexandrinum)Hay and Cellulase Activity (2007)
  • Author(s): El-waziry, A.M. and Ibrahim, H.R.
  • Journal Title: Australian Journal of Basic and Applied Sciences 1(4) Pages: 379-385
  • Peer Reviewed
• [Journal Article] Ovotransferrin possesses SOD-like superoxide anion scavenging activity that is promoted by copper and manganese binding (2007)
  • Author(s): Ibrahim, H.R.; Hoq, M.I; Aoki, T.
  • Journal Title: Intl. J. Biol. Macromol. 41 Pages: 631-640
  • Peer Reviewed
• [Journal Article] Effect of Saccharomyces cerevisiae on cell wall constituents digestion in sheep fed Berseem(Trifolium alexandrinum)hay and cellulase activity (2007)
  • Author(s): El-waziry, A.M.; Ibrahim, H.R.
  • Journal Title: Australian Journal of Basic and Applied Sciences 1 Pages: 379-385
  • Peer Reviewed
• [Journal Article] Ovotransferrin is a Redox-dependent Autoprocessing Protein Incorporating Four Consensus Self-cleaving Motifs Flanking the Two Kringles (2006)
  • Author(s): Ibrahim, H.R.; Haraguchi, T.; Aoki, T.
  • Journal Title: Biochim. Biophys. Acta 1760(3) Pages: 347-355
  • Peer Reviewed
• [Journal Article] Ovotransferrin is a redox-dependent autoprocessing protein incorporating four consensus selfcleaving motifs flanking the two kringles (2006)
  • Author(s): Ibrahim, H.R., Haraguchi, T., Aoki, T.
  • Journal Title: Biochim. Biophys. Acta 1760 Pages: 347-355
• [Journal Article] Antimicrobial peptides derived from hen egg lysozyme with inhibitory effect against Bacillus species (2006)
  • Author(s): Abdou, A.M., Higashiguchi, S., Abou eleinin, A.M., Kim, M., Ibrahim.H.R.
  • Journal Title: Food Control (in press)
• [Presentation] Squeezing New Therapeutic PeptidesOut of Egg Albumin (2009)
  • Author(s): Hisham Ibrahim
  • Organizer: 第100回 AOCSのBioactive Peptides in Human Health and Diseases国際シンポジウム
  • Place of Presentation: 国際、Orlando, USA
  • Year and Date: 2009-05-05
• [Presentation] ヒトリゾチーム由来抗菌ペプチドの発見 (2009)
  • Author(s): Hisham Ibrahim
  • Organizer: 日本農芸化学会ー2009年度大会
  • Place of Presentation: 国内、福岡市
  • Year and Date: 2009-03-28
• [Presentation] リゾチームとトリクロサンの相互作用とその抗菌活性及び抗酸化機能 (2009)
  • Author(s): Hisham Ibrahim、イムラヌル ホク
  • Organizer: 日本農芸化学会ー2009年度大会
  • Place of Presentation: 国内、福岡市
  • Year and Date: 2009-03-28
• [Presentation] 体内消化シミュレーションによる卵白の機能性ペプチドの解明 (2009)
  • Author(s): Hisham Ibrahim、 中野彰宣
  • Organizer: 日本農芸化学会ー2009年度大会
  • Place of Presentation: 国内、福岡市
  • Year and Date: 2009-03-28
• [Presentation] サツマイモ発酵に共存する新規プロバイオティクスの抗菌および抗酸化機能の解明 (2009)
  • Author(s): Hisham Ibrahim、 藤岡観
  • Organizer: 日本農芸化学会ー2009年度大会
  • Place of Presentation: 国内、福岡市
  • Year and Date: 2009-03-28
• [Presentation] Multiplex Biopeptides of C-type Lysozymes : Emerging Therapeutic Potential (2009)
  • Author(s): Hisham Ibrahim
  • Organizer: 第237回 ACSのBiopeptides Disease Risk Reduction 国際シンポジウム
  • Place of Presentation: 国際、Salt Lake, USA
  • Year and Date: 2009-03-23
• [Presentation] Multiplex Biopeptides of C-type Lysozymes : Emerging Therapeutic Potential (2009)
  • Author(s): Ibrahim. H. R.
  • Organizer: International Symposium “Biopeptides and Specialty Proteins for Health Promotion and Disease Risk Reduction"
  • Place of Presentation: ソルトレーク(米国)
• [Presentation] Discovery of Functional Sectrets of Hen's Egg Ovotransferrin : A New Potential for Human Health (2008)
  • Author(s): Hisham Ibrahim
  • Organizer: 第99回AOCSのAdvances and Challenges toward Health Benefits of Proteins 国際シンポジウム
  • Place of Presentation: 国際、Salt Lake,USA
  • Year and Date: 2008-05-21
• [Presentation] The Discovery of Functional Sectrets of Hen's Egg Ovotransferrin : A New Potential for Human Health (2008)
  • Author(s): Ibrahim. H. R.
  • Organizer: International Symposium “Advances and Challenges toward Health Benefits of Proteins"
  • Place of Presentation: シアトル(米国)
• [Presentation] オボトランスフェリンの機能ミステリーの解明 : ヒト治療のために (2006)
  • Author(s): Hisham Ibrahim
  • Organizer: 第3回食品蛋白質 シンポジウム
  • Place of Presentation: 国内、山口市
  • Year and Date: 2006-08-19
• [Presentation] Strategy for Novel Anti-infection and Anti-oxidant Polyphenols-protein Complex : An example with Lysozyme
  • Author(s): Hisham Ibrahim
  • Organizer: 第98回AOCSののタンパク質およびその生成物質 国際シンポジウム
  • Place of Presentation: 国際、Quebec, Canada
• [Patent(Industrial Property Rights)] リゾチームおよびトリクロサンの新規抗菌剤の製造方法 (2008)
  • Inventor(s): ヒッシャムラドワンイブラヒム
  • Industrial Property Rights Holder: 株式会社マンダム
  • Filing Date: 2008-09-10
• [Patent(Industrial Property Rights)] 抗菌組成物、その製造方法及び該抗菌組成物を含有する抗菌剤 (2008)
  • Inventor(s): ヒッシャムラドワン イブラヒム
  • Industrial Property Rights Holder: 株式会社ファーマフーズ
  • Acquisition Date: 2008-11-21
• [Patent(Industrial Property Rights)] 抗菌剤およびその製造方法、化粧料ならびに医薬品 (2008)
  • Inventor(s): ヒッシャムラドワン イブラヒム三野浩二 辻野義雄
  • Industrial Property Rights Holder: 国立大学法人鹿児島大学と株式会社マンダム
  • Filing Date: 2008-09-10