| Project/Area Number |
18591085
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Ehime University (2008-2009)
Dokkyo Medical University (2006-2007) |
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Principal Investigator |
EGUCHI Mariko Ehime University, 医学部・附属病院, 講師 (40420781)
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| Co-Investigator(Kenkyū-buntansha) |
EGUCHI Minenori 愛媛大学, 医学系研究科, 講師 (50420782)
YAMAGATA Tetsuya 獨協医科大学, 医学部, 准教授 (30424047)
MAKI Kazuhiro 獨協医科大学, 医学部, 講師 (50337391)
SASAKI Ko 獨協医科大学, 医学部, 講師 (60282638)
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| Project Period (FY) |
2006 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,360,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥660,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | TEL / ETV6 / TEL-TRKC / 転写因子 / 造血細胞分化 / ES細胞 / トランスジェニックマウス / 赤血球分化 / 巨核球分化 |
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| Research Abstract |
Murine embryonic stem (ES) cells expressing wild type TEL and TEL-TRKC fusion gene, identified as an oncogenic factor in both leukemia and infantile fibrosarcoma, were established. The ES cells were differentiated into hematopoietic cells and analyzed in detail to clarify its function necessary for tumorigenesis. ES cells expressing wild type TEL had the tendency to remain in immature hematopoietic cells, whereas TEL-TRKC expressing ES cells could not differentiate into hemangioblast, a common precursor of endothelial and blood cells, and their capacity to produce hematopoietic stem cells were severely impaired. Mesoderm derived, immature cells increased instead of blood cells, which might be the cancer stem cells for TEL-TRKC harboring fibrosarcoma. Interacting protein to TEL is now being investigated for its association with tumorigenesis.
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