Effects of relaxin on collagen metabolism by human periodontal ligament cells

• Project/Area Number: 18592252
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Nihon University
• Principal Investigator: YAMAGUCHI Masaru Nihon University, 松戸歯学部, 准教授 (60333100)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,060,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥660,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: Relaxin / ヒト歯根膜線維芽細胞 / 細胞外マトリックス代謝 / 伸展力 / relaxin / 細胞外マトリックス

Research Abstract

伸展された歯根膜細胞にrelaxin刺激を行った結果, Col-Iタンパク産生量およびmRNA発現量はrelaxin濃度依存的に減少が認められたが, MMP-1の発現量は濃度依存的に増加が認められた。relaxinが伸展されたヒト歯根膜細胞におけるCol-IとMMP-1の発現に影響を与えたことより, このホルモンが矯正治療後の後戻りを防止するのに有効である可能性が示唆された

Research Products

• [Journal Article] Relaxin modulates collagen type I and metalloproteinase-1 expression by human periodontal ligament cells (2008)
  • Author(s): Takano M, Yamaguchi M, Nakajima R, Kojima T, Kasai K.
  • Journal Title: J Oral Bioscience 50(4) Pages: 222-229
  • Peer Reviewed
• [Journal Article] Immunohistochemical localization of relaxin in rat periodontal ligament, dental pulp tissue, and midpalatal suture (2008)
  • Author(s): Yamaguchi M, Kubo R, Utsunomiya T, Yamamoto H, Kasai K.
  • Journal Title: Int J Oral-Med Sci 7 Pages: 35-39
  • NAID: 130000411391
• [Journal Article] Immunohistochemical localization of relaxin in rat periodontal ligament, dental pulp tissue, and midpalatal suture (2008)
  • Author(s): Yamaguchi M, Kubo R, Utsunomiva T. Yamamoto H., et al.
  • Journal Title: Int J Oral-Med Sci 7 Pages: 35-39
  • NAID: 130000411391
  • Peer Reviewed
• [Journal Article] Relaxin modulates collagen type I and metalloproteinase-1expression by human periodontal ligament cells (2008)
  • Author(s): Takano M, Yamaguchi M, Nakajima R, Kojima T, Kasai K.
  • Journal Title: J Oral Bioscience 50 (4) Pages: 222-229
  • Peer Reviewed
• [Journal Article] Effects of compression force on fibroblast growth factor-2 and receptor activator of nuclear factor kappa B ligand production by periodontal ligament cells in vitro. (2008)
  • Author(s): Nakajima R, Yamaguchi M, Kojima T, Takano M, Kasai K.
  • Journal Title: Journal of Periodontal Research 43 Pages: 168-173
  • Peer Reviewed
• [Journal Article] The effects of orthodontic mechanics on the dental pulp. (2007)
  • Author(s): Yamaguchi M, Kasai K.
  • Journal Title: Seminars in Orthodontics 13 Pages: 272-280
  • Peer Reviewed
• [Journal Article] Effects of relaxin on collagen type I released by stretched human periodontal ligament cells
  • Author(s): Takano M, Yamaguchi M, Nakajima R, Fujita S, Kojima T, Kasai K.
  • Journal Title: Orthod Craniofacial Res (in press)
  • Peer Reviewed
• [Journal Article] Histopathological Study of Inhibitory Effects for Relapse by Relaxin after Expansion in Rat Midpalatal Suture
  • Author(s): Kubo R, Utsunomiya T, Yamaguchi M.
  • Journal Title: Int J Oral-Med Sci (in press)
  • NAID: 130000411431
  • Peer Reviewed
• [Presentation] Relaxinがヒト歯根膜由来細胞のコラーゲン代謝に及ぼす影響 (2008)
  • Author(s): 高野真知、山口大、中嶋亮、藤田祥仁、児島格、葛西一貴,
  • Organizer: 第50回歯科基礎医学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2008-09-23
• [Presentation] Relaxin modulates collagen type I and matrix metalloproteinase-1 expression by human periodontal ligament cells (2008)
  • Author(s): Takano M, Yamaguchi M, Nakajima R, Kojima T, Kasai K.
  • Organizer: 86th General Session and Exhibition of the International Association for Dental Reserch
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2008-07-04
• [Presentation] Relaxin modulates collagen type I and matrix metalloproteinase-1 expression by human periodontal ligament cells. (2008)
  • Author(s): Takano M, Yamaguchi M, Nakajima R, Kojima T, Kasai K
  • Organizer: 86th General Session and Exhibition of the International Association for Dental Reserch
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2008-07-04
• [Presentation] ヒト歯根膜由来細胞におけるLGR7、LGR8発現の検討 (2007)
  • Author(s): 高野 真知、中嶋 亮、児島 格 山口 大、葛西 一貴
  • Organizer: 第66回日本矯正歯科学会大会
  • Place of Presentation: 大阪
  • Year and Date: 2007-09-21
• [Presentation] ヒト歯根膜由来細胞におけるLGR7,LGR8発現の検討 (2007)
  • Author(s): 高野真知、中嶋亮、児島格、山口大、葛西一貴
  • Organizer: 第66回日本矯正歯科学会大会
  • Place of Presentation: 大阪
  • Year and Date: 2007-09-20
• [Presentation] ラット正中口蓋縫合拡大によるrelaxin発現とType I collagen 発現の変化について (2007)
  • Author(s): 久保利枝、山口大、宇都宮忠彦、山本浩嗣、葛西一貴
  • Organizer: 第66回日本矯正歯科学会大会
  • Place of Presentation: 大阪
  • Year and Date: 2007-09-20
• [Presentation] ラット正中口蓋縫合拡大によるrelaxin発現の変化について (2006)
  • Author(s): 久保利枝、山口大, 兼川美香, 宇都宮忠彦、山本浩嗣、葛西一貴
  • Organizer: 第65回日本矯正歯科学会大会
  • Place of Presentation: 札幌
  • Year and Date: 2006-09-14