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Identification of Streptoooocus gordoniigenes involved in recruiting Porphyromonas gingivatiscens into a multispecies biofilm

Research Project

Project/Area Number 18592276
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Social dentistry
Research InstitutionOsaka University

Principal Investigator

KUBONIWA Masae  Osaka University, Department of Preventive Dentistry, Assistant Professor (00303983)

Co-Investigator(Kenkyū-buntansha) NAGATA Hideki  Osaka University, Department of Preventive Dentistry, Associate Professor (50260641)
KATAOKA Kosuke  Osaka University, Department of Preventive Dentistry, Assistant Professor (50283792)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,730,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2006: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsStreptococcus gordonii / Porphyromones gingivalis / biofilm / inter cellular signaling / intra cellular signaling / capsule / cell wall / quorum sensing / Fusobacterium nucleatum
Research Abstract

Dental plaque biofilm formation proceeds through a developmental pathway initiated by the attachment of pioneer organisms, such as S. gordonii, to tooth surfaces. Through a variety of synergistic interactions, pioneer organisms facilitate the colonization of later arrivals including P. gingivalis, a potential periodontal pathogen. We have investigated genes of S. gordonii required to support a heterotypic biofilm- community with P gingivalis. By screening a plasmid integration library of S gordonii, genes were identified that are crucial for the accumulation of planktonic R gingivalis cells into a multispecies biofilm. These genes were further investigated by specific mutation and complementation analyses. The biofilm-associated genes can be grouped into broad categories based on putative function as follows: i) intercellular or intracellular signaling (cbe and spxB), ii) cell wall integrity and maintenance of adhesive proteins (murE, msrA and atf), iii) extracellular capsule biosynthesis (pgsA and atf), iv) physiology (gdhA, ccmA and ntpB). In addition, a gene for a hypothetical protein was identified. Biofilm visualization and quantitation by confocal microscopy confirmed the role of these genes in the maturation of the multispecies community, including biofilm architectural development. The results suggest that S. gordonii governs the development of heterotypic oral biofilms through multiple genetic pathways.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (44 results)

All 2008 2007 2006

All Journal Article (24 results) (of which Peer Reviewed: 11 results) Presentation (20 results)

  • [Journal Article] Heterogenic virulence and related factors among clinical isolates of Porphyromonas gingivalis with type II fimbriae.2008

    • Author(s)
      Inaba. H., et. al.
    • Journal Title

      Oral Microbiology and Immunology 23

      Pages: 29-35

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] P. gingivalis accelerates gingival epithelial cell progression through the cell cycle.2008

    • Author(s)
      Kuboniwa, M., et. al.
    • Journal Title

      Microbes and Infection 10

      Pages: 122-128

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Porphyromonas gingivalis accelerates gingival epithelial cell progression through the cell cycle2008

    • Author(s)
      Kuboniwa, M
    • Journal Title

      Microbes Infect 10

      Pages: 122-128

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Heterogenic virulence and related factors among clinical isolates of Porphyromonas gingivalis with type II fimbriae2008

    • Author(s)
      Inaba, H
    • Journal Title

      Oral Microbiol Immunol 23

      Pages: 29-35

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] P. gingivalis accelerates gingival epithelial cell progression through the cell cycle.2008

    • Author(s)
      Kuboniwa, M., Y. Hasegawa, S. Mao, S. Shizukuishi, A. Amano, R. J. Lamont, and O. Yilmaz.
    • Journal Title

      Microbes and Infection 10

      Pages: 122-128

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Heterogenic virulence and related factors among clinical isolates of Porphyromonas gingivalis with type II fimbriae.2008

    • Author(s)
      Inaba, H., K. Nakano, T. Kato, R. Nomura, S. Kawai, M. Kuboniwa, K. Ishihara, T. Ooshima, and A. Amano.
    • Journal Title

      Oral Microbiology and Immunology 23

      Pages: 29-35

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Virulence of Porphyromonas gingivalis is altered by substitution of fimbria gene with different genotype.2007

    • Author(s)
      Kato, T., et. al.
    • Journal Title

      Cellular Microbiology 9

      Pages: 753-765

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Longitudinal study of the association between smoking as a periodontitis risk and salivary biomarkers related to periodontitis.2007

    • Author(s)
      Kibayashi, M., et. al.
    • Journal Title

      Journal of Periodontology 78

      Pages: 859-867

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Longitudinal study of the association between smoking as a periodontitis risk and salivary biomarkers related to periodontitis2007

    • Author(s)
      Kibayashi, M
    • Journal Title

      J Periodontol 78

      Pages: 859-867

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Virulence of Porphyromonas gingivalis is altered by substitution of fimbria gene with different genotype2007

    • Author(s)
      Kato, T
    • Journal Title

      Cell Microbiol 9

      Pages: 753-765

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Longitudinal study of the association between smoking as a periodontitis risk and salivary biomarkers related to periodontitis.2007

    • Author(s)
      Kibayashi, M., M. Tanaka, N. Nishida, M. Kuboniwa, K. Kataoka, H. Nagata, K. Nakayama, K. Morimoto, and S. Shizukuishi.
    • Journal Title

      Journal of Periodontology 78

      Pages: 859-867

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Virulence of Porphyromonas gingivalis is altered by substitution of fimbria gene with different genotype.2007

    • Author(s)
      Kato, T., S.Kawai, K.Nakano, H.Inaba, M.Kuboniwa, I.Nakagawa, K.Tsuda, H.Omori, T.Ooshima, T.Yoshimori, A.Amano.
    • Journal Title

      Cellular Microbiology 9

      Pages: 753-765

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Streptococcus gordonii utilizes several distinct gene functions to recruit Porphyromonas gingivalis into a mixed community.2006

    • Author(s)
      Kuboniwa, M., et. al.
    • Journal Title

      Molecular Microbiology 60

      Pages: 121-139

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Role of the Porphyromonas gingivalis InlJ protein in homotypic and heterotypic biofilm development.2006

    • Author(s)
      Capestany, C. A., et. al.
    • Journal Title

      Infection and Immunity 74

      Pages: 3002-3005

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] LuxS involvement in the regulation of genes coding for hemin and iron acquisition systems in Porphyromonas gingivalis2006

    • Author(s)
      James, C. E., et. al.
    • Journal Title

      Infection and Immunity 74

      Pages: 3834-3844

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Porphyromonas gingivalis Genes Involved in Community Development with Streptococcus gordonii.2006

    • Author(s)
      Simionato, M. R., et. al.
    • Journal Title

      Infection and Immunity 74

      Pages: 6419-6428

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Porphyromonas gingivalis genes involved in community development with Streptococcus gordonii2006

    • Author(s)
      Simionato, M R
    • Journal Title

      Infect Immun 74

      Pages: 6419-6428

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] LuxS involvement in the regulation of genes coding for hemin and iron acquisition systems in Porphyromonas gingivalis2006

    • Author(s)
      James, C K
    • Journal Title

      Infect Immun 74

      Pages: 3834-3844

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Role of the Porphyromonas gingivalis In1J protein in homotypic and heterotypic biofilm development2006

    • Author(s)
      Capestany, C A
    • Journal Title

      Infect Immun 74

      Pages: 3002-3005

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Streptococcus gordonii utilizes several distinct gene functions to recruit Porphyromonas gingivalis into a mixed community2006

    • Author(s)
      Kuboniwa, M
    • Journal Title

      Mol Microbiol 60

      Pages: 121-139

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Streptococcus gordonii utilizes several distinct gene functions to recruit Porphyromonas gingivalis into a mixed community.2006

    • Author(s)
      Kuboniwa, M., G.D.Tribble, C.E.James, A.O.Kilic, L.Tao, M.C.Herzberg, S.Shizukuishi, R.J.Lamont.
    • Journal Title

      Molecular Microbiology 60

      Pages: 121-139

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Role of the Porhyromonas gingivalis InIJ protein in homotypic and heterotypic biofilm development.2006

    • Author(s)
      Capestany, C.A., M.Kuboniwa, I.Y.Jung, Y.Park, G.D.Tribble, R.J.Lamont.
    • Journal Title

      Infection and Immunity 74

      Pages: 3002-3005

    • Related Report
      2006 Annual Research Report
  • [Journal Article] LuxS involvement in the regulation of genes coding for hemin and iron acquisition systems in Porphyromonas gingivalis2006

    • Author(s)
      James, C.E., Y.Hasegawa, Y.Park, V.Yeung, G.D.Tribble, M.Kuboniwa, D.R.Demuth, R.J.Lamont.
    • Journal Title

      Infection and Immunity 74

      Pages: 3834-3844

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Porphyromonas gingivalis Genes Involved in Community Development with Streptococcus gordonii.2006

    • Author(s)
      Simionato, M.R., C.M.Tucker, M.Kuboniwa, G.Lamont, D.R.Demuth, G.D.Tribble, R.J.Lamont.
    • Journal Title

      Infection and Immunity 74

      Pages: 6419-6428

    • Related Report
      2006 Annual Research Report
  • [Presentation] Effect of genotypes of Porphyromonas gingivalis fimbriae on biofilm for mation2007

    • Author(s)
      Kuboniwa, M., et. al.
    • Organizer
      第55回 国際歯科研究学会日本部会(JADR)総会・学術大会
    • Place of Presentation
      鶴見大学記念館
    • Year and Date
      2007-11-18
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Sugar alcohols alter periodontopathic biofilm development and vulnerability2007

    • Author(s)
      Hashino, E., et. al.
    • Organizer
      第55回 国際歯科研究学会日本部会(JADR)総会・学術大会
    • Place of Presentation
      鶴見大学記念館
    • Year and Date
      2007-11-18
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Inhibitory effect of human GAPDE peptide on Porphyromonas gingivalisinvasion2007

    • Author(s)
      Iwasaki, M., et. al.
    • Organizer
      第55回 国際歯科研究学会日本部会(JADR)総会・学術大会
    • Place of Presentation
      鶴見大学記念館
    • Year and Date
      2007-11-18
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effect of genotypes of Porphyromonas gingivalis fimbriae on biofilm formation2007

    • Author(s)
      Kuboniwa, M., A. Amano, T. Kato, M. Iwasaki, E. Hashino and S. Shizukuishi
    • Organizer
      第55回 国際歯科研究学会日本部会(JADR)総会・学術大会
    • Place of Presentation
      鶴見大学記念館
    • Year and Date
      2007-11-18
    • Related Report
      2007 Annual Research Report
  • [Presentation] Inhibitory effect of human GAPDH peptide on Porphyromonas gingivalis invasion2007

    • Author(s)
      Iwasaki, M., M. Kuboniwa, H. Nagata, E. Hashino, N. Nishida and S. Shizukuishi
    • Organizer
      第55回 国際歯科研究学会日本部会(JADR)総会・学術大会
    • Place of Presentation
      鶴見大学記念館
    • Year and Date
      2007-11-18
    • Related Report
      2007 Annual Research Report
  • [Presentation] Porphyromonas gingivalis accelerates cell cycle progression in gingival epithelial cells2007

    • Author(s)
      Kuboniwa, M., et. al.
    • Organizer
      85th General Session and Exhibition of the IADR/AADR/CADR
    • Place of Presentation
      New Orleans, LA, USA
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effect of genotypes of Porphyromonas gingivalis fimbriae on biofilm formation2007

    • Author(s)
      Kuboniwa, M
    • Organizer
      55th Annual Meeting of Japanese Association for Dental Research
    • Place of Presentation
      Yokohama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Sugar alcohols alter periodontopathic biofilm development and vulnerability2007

    • Author(s)
      Hashino, E
    • Organizer
      55th Annual Meeting of Japanese Association for Dental Research
    • Place of Presentation
      Yokohama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Inhibitory effect of human GAPDH peptide on Porphyromonas gingivalis invasion2007

    • Author(s)
      Iwasaki, M
    • Organizer
      55th Annual Meeting of Japanese Association for Dental Research
    • Place of Presentation
      Yokohama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Porphyromonas gingivalis accelerates cell cycle progression in gingival epithelial cells2007

    • Author(s)
      Kuboniwa, M
    • Organizer
      85th General Session & Exhibition of the IADR
    • Place of Presentation
      New Orleans
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The Clp Stress Response System and Porphyromonas gingivalis Invasion2007

    • Author(s)
      Capestany, C A
    • Organizer
      85th General Session & Exhibition of the IADR
    • Place of Presentation
      New Orleans
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 予防歯科診療における禁煙支援および誘導症例の検討2006

    • Author(s)
      山上千夏, ら
    • Organizer
      第17回日本口腔衛生学会近畿・中国・四国地方会総会
    • Place of Presentation
      和歌山県歯科医師会館
    • Year and Date
      2006-06-18
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] An examination case of a program in dental clinic to support patients to stop smoking2006

    • Author(s)
      Yamagami, C
    • Organizer
      17th regional meeting of Japanese Society for Dental Health
    • Place of Presentation
      Wakayama
    • Year and Date
      2006-06-18
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Streptococcus gordonii Uses Several Distinct Gene Functions to Recruit Porphyromonas gingivalis to Biofilms.2006

    • Author(s)
      Kuboniwa, M., et. al.
    • Organizer
      106th General Meeting of the American Society for Microbiology
    • Place of Presentation
      Orlando, FL, USA
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] P. gingivalis Interactions with Human Placental Cells and Tissues2006

    • Author(s)
      Katz, J., et. al.
    • Organizer
      84th General Session & Exhibition of the IADR
    • Place of Presentation
      Brisbane, Australia
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Longitudinal Study of Association of Alcohol Consumption and ALDH2 Genotypes wity Periodontal Disease Progression2006

    • Author(s)
      Tanaka, M., et. al.
    • Organizer
      7 th Congress of Asian Academy of Preventive Dentistry
    • Place of Presentation
      Okayama, Japan
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Analysis of Porphyromonas gingivalis Genes Involved in Mixed Biofilm Formation2006

    • Author(s)
      Tucker, C M
    • Organizer
      35th Annual Meeting and Exhibition of the AADR
    • Place of Presentation
      Orlando
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Streptococcus gordonii Uses Several Distinct Gene Functions to Recruit Porphyromonas gingivalis to Biofilms2006

    • Author(s)
      Kuboniwa, M
    • Organizer
      106th General Meeting of the ASM
    • Place of Presentation
      Orlando
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] gingivalis Interactions with Human Placental Cells and Tissues2006

    • Author(s)
      Katz, J P
    • Organizer
      84th General Session & Exhibition of the IADR
    • Place of Presentation
      Brisbane
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Longitudinal Study of Association of Alcohol Consumption and ALDH2 Genotypes with Periodontal Disease Progression2006

    • Author(s)
      Tanaka, M
    • Organizer
      7th Congress of Asian Academy of Preventive Dentistry
    • Place of Presentation
      Okayama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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