Biomimetic fabrication of biomaterials using cell manipulation

• Project/Area Number: 18680039
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Osaka University
• Principal Investigator: MATSUMOTO Takuya Osaka University, 大学院・歯学研究科, 講師 (40324793)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥27,820,000 (Direct Cost: ¥21,400,000、Indirect Cost: ¥6,420,000); Fiscal Year 2008: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2007: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2006: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
• Keywords: バイオミメティック / 有機・無機複合材料 / ハイドロキシアパタイト / 細胞機能制御 / アミノ酸 / 生体吸収性 / 結晶成長

Research Abstract

本研究では生体内における硬組織発生を模倣したアプローチにより、無機単体、無機/有機複合体からなる新規機能性スキャフォールドの作製、また、スキャフォールド内における細胞の増殖、分化、三次元パターニングさらには組織化といった細胞操作をあらかじめin vitroにて行うことで無機/有機/細胞からなる生体組織様組織(プレティッシュ)の構築を試みた。その結果、塩基性タンパク質に対して親和性の高いアパタイト材料や含有アパタイト量が傾斜的に変化するゲル材料の作製に成功した。さらに、ゲル内細胞に対する外的刺激を応用することで、ゲル内細胞の配置、配向制御を達成し、これら細胞含有ゲルを利用した石灰化誘導により、細胞/有機/無機からなる三次元複合材料の作製を達成した。本研究で作製した材料は新しい硬組織再生、再建用材料として有望である。また、本研究で示したような生体擬似的アプローチによる材料の作製は新しい機能性材料創製において有効なシステムとなりうる。

Research Products

• [Journal Article] Biomimetic fabrication of fibrin/apatite composite material (2008)
  • Author(s): Yoh R, Matsumoto T, Sasaki J, Sohmura T
  • Journal Title: J Biomed Mater Res A 87 Pages: 222-228
• [Journal Article] Clinical evaluation of the efficacy of removing microorganisms to disinfect patient-derived dental impressions. (2008)
  • Author(s): Egusa H, Watamoto T, Matsumoto T, Abe K, Kobayashi M, Akashi Y, Yatani H
  • Journal Title: Int J Prosthodont. 21 Pages: 531-538
• [Journal Article] An analysis of the persistent presence of opportunistic pathogens on patient-derived dental impressions and gypsum casts. (2008)
  • Author(s): Egusa H, Watamoto T, Abe K, Kobayashi M, Kaneda Y, Ashida S, Matsumoto T, Yatani H
  • Journal Title: Int J Prostho 21 Pages: 62-68
• [Journal Article] Biomimetic fabrication of fibrin/apatite composite material (2008)
  • Author(s): Yoh R, Matsumoto T, Sasaki J, Sohmura T.
  • Journal Title: J Biomed Mater Res A 87 Pages: 222-228
  • Peer Reviewed
• [Journal Article] Functional control of myoblasts in a strained fibrin gel (2008)
  • Author(s): Matsumoto T, Sasaki J, Uddin MH, Egusa H, Sohmura T.
  • Journal Title: Tissue Eng part A 14 Pages: 737-737
  • Peer Reviewed
• [Journal Article] An analysis of the persistent presence of opportunistic pathogens on patient-derived dental impressions and gypsuin casts (2008)
  • Author(s): Egusa H, Watamoto T, Abe K, Kobayashi M, Kaneda Y, Ashida S, Matsumoto T, Yatani H.
  • Journal Title: Int J Prostho 21 Pages: 62-68
  • Peer Reviewed
• [Journal Article] Biomimetic fabrication of fibrin/apatite composite material. (2008)
  • Author(s): Yoh R, Matsumoto T, Sasaki J, Sohmura T.
  • Journal Title: J Biomed Mater Res A in press
  • Peer Reviewed
• [Journal Article] Three-dimensional cell and tissue patterning in a strained fibrin gel system (2007)
  • Author(s): Matsumoto T, Sasaki J, Alsberg E, Egusa H, Yatani H, Sohmura T
  • Journal Title: PLoS ONE 2
• [Journal Article] Engineering tumors with 3D scaffolds (2007)
  • Author(s): Fischbach C, Chen R, Matsumoto T, Schmelzle T, Brugge J, Polverini P, Mooney DJ
  • Journal Title: Nat Methods 4 Pages: 855-860
• [Journal Article] Mechanical strain regulates endothelial cell patterning in vitro (2007)
  • Author(s): Matsumoto T, Yung YC, Fischbach C, Kong HJ, Nakaoka R, Mooney DJ
  • Journal Title: Tissue Eng 13 Pages: 207-217
• [Journal Article] Modification of apatite materials for bone tissue engineering and drug delivery system (2007)
  • Author(s): Matsumoto T, Okazaki M, Nakahira A, Sasaki J, Egusa H, Sohmura T
  • Journal Title: Curr Med Chem 14 Pages: 2726-2733
• [Journal Article] Synthetic osteopontin-derived peptide SVVYGLR induce neovascularization in artificial one marrow scaffold materials (2007)
  • Author(s): Hamada Y, Egusa H, Kaneda Y, Hirata I, Kawaguchi N, Hirao T, Matsumoto T, Yao M, Daito K, Yatani H, Okazaki M, Matsuura N
  • Journal Title: Dent Mater J 26 Pages: 487-492
• [Journal Article] Engineering tumors with 3D scaffolds. (2007)
  • Author(s): Fischbach C, Chen R, Matsumoto T, Schmelzle T, Brugge J, Polverini P, Mooney DJ.
  • Journal Title: Nat Methods 4 Pages: 855-860
  • Peer Reviewed
• [Journal Article] Moddiffcation of apatite materials for bone tissue engineering and drug delivery system. (2007)
  • Author(s): Matsumoto T, Okazaki M, Nakahira A, Sasaki J, Egusa H, Sohmura T.
  • Journal Title: Curr Med Chem 14 Pages: 2726-2733
  • Peer Reviewed
• [Journal Article] Synthetic osteopontin-derived peptide SVVYGLR induce neovascularization in artificial one marrow scaffold materials (2007)
  • Author(s): Hamada Y, Egusa H, Kaneda Y, Hirata I, Kawaguchi N, Hirao T, Matsumoto T, Yao M, Daito K, Yatani H, Okazaki M, Matsuura N.
  • Journal Title: Dent Mater J 26 Pages: 187-492
  • Peer Reviewed
• [Journal Article] Different behavior of implanted hydroxyapatite depending on its size and crystallinity. (2006)
  • Author(s): Matsumoto T, Tamine K, Kagawa R, Hamada Y, Okazaki M, Takahashi J
  • Journal Title: J Ceram Soc Jap 114 Pages: 760-762
• [Journal Article] Role of acidic amino acid for regulating hydroxyapatite crystal growth. (2006)
  • Author(s): Matsumoto T, Okazaki M, Inoue M, Sasaki J, Hamada Y, Takahashi J
  • Journal Title: Dent Mater J 25 Pages: 360-364
  • NAID: 110004757990
• [Journal Article] New concept bioceramics composed of octacalcium phosphate (OCP) and dicarboxylic acid intercalated OCP via hydrothermal hot-pressing.
  • Author(s): Ishihara S, Matsumoto T, Onoki T, Sohmura T, Nakahira A
  • Journal Title: Mater Sci Eng C in press
• [Journal Article] New concept bioceramics composed of octacalcium phosphate(OCP)and dicarboxylic acid intercalated OCP via hydrothermal hot-pressing
  • Author(s): Shiho Ishihara, Takamasa Onoki, Takuya Matsumoto, Taiji Sohmura, Atsushi Nakahira
  • Journal Title: Mater Eng Sci C (In press)
  • Peer Reviewed
• [Presentation] Synthesis and characterization of hydroxyapatite containing glycine and serine (2008)
  • Author(s): Mohammad Hafiz UDDIN, Takuya MATSUMOTO, Taiji SOHMURA
  • Organizer: 日本歯科理工学会
  • Place of Presentation: 大阪
  • Year and Date: 2008-09-20
• [Presentation] Biomimetic cell containing scaffold for bone tissue engineering (2008)
  • Author(s): Ami Mizuno, Takuya Matsumoto, Shinsuke Yoshida, Jun-Ichi Sasaki, Taiji Sohmura
  • Organizer: IADR
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2008-07-04
• [Presentation] Modification of mechanical properties of fibrin gel. (2008)
  • Author(s): Kashiwagi M, Matsumoto T, Sasaki J, Sohmura T
  • Organizer: IADR
  • Place of Presentation: Toronto, Canada
• [Presentation] Function change of osteoblasts subjected to different mechanical stimulation (2008)
  • Author(s): Sasaki J, Matsumoto T, Egusa H, Sohmura T, Yatani H
  • Organizer: IADR
  • Place of Presentation: Toronto, Canada
• [Presentation] フィブリンゲル内での生体擬似的石灰化 (2007)
  • Author(s): 松本卓也
  • Organizer: 日本バイオマテリアル学会
  • Place of Presentation: 大阪
  • Year and Date: 2007-11-26
• [Presentation] 三次元ハイドロゲルを用いた骨芽細胞配向、機能制御 (2007)
  • Author(s): 佐々木 淳一、松本 卓也、江草宏、荘村 泰治、矢谷 博文
  • Organizer: 日本バイオマテリアル学会
  • Place of Presentation: 大阪
  • Year and Date: 2007-11-26
• [Presentation] Characterization of hydroxyapatite containing aspartic acid (2007)
  • Author(s): Uddin MH, Matsumoto T, Sohmura T.
  • Organizer: International Dental Materials Congress
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2007-11-24
• [Presentation] Control of patterning, patterning, proliferation, and differentiation of myoblasts in strained fibrin gels. (2007)
  • Author(s): Matsumoto T, Sasaki J, Egusa H. Sohmura T.
  • Organizer: Biomedical Engineering Society 2007 Fall Meeting
  • Place of Presentation: Los Angels, USA
  • Year and Date: 2007-09-28
• [Book] 生命歯科医学のカッティングエッジ 米田俊之編 (2008)
  • Author(s): 荘村泰治、寺岡文雄、松本卓也.
  • Publisher: 新たな修復用材料の造形とその表面修飾.
• [Book] Cell Instructive Polymers. "Tissue Engineering" Advances in Biochemical Engineering/Biotechnology series. Edited (2006)
  • Author(s): Matsumoto T, Mooney DJ
  • Publisher: Kaplan D. Springer, Berlin
• [Book] Advances in Biochemical Engineering/Biotechnology series. (2006)
  • Author(s): Matsumoto T, Mooney DJ
  • Total Pages: 243
  • Publisher: Cell Instructive Polymers
• [Remarks]
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• [Remarks]
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