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Investigation of membrane protein functions by changing curvature and asymmetric lipid membrane of vesicles

Research Project

Project/Area Number 18H02092
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 37010:Bio-related chemistry
Research InstitutionGunma University

Principal Investigator

Koki Kamiya  群馬大学, 大学院理工学府, 助教 (70612315)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2020: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Keywordsリポソーム / 人工細胞膜 / マイクロデバイス / リン脂質非対称膜 / 人工細胞モデル / ガングリオシド / 膜タンパク質 / 人工細胞膜モデル / エキソサイトーシス / ベシクル
Outline of Final Research Achievements

Cell membranes of eukaryotic cells were composed of an asymmetric lipid bilayer. In this study, to understand biological phenomena involved in the asymmetric lipid bilayer, we generated an automatic device of cell-sized asymmetric lipid vesicles, nano-sized asymmetric lipid vesicles, vesicles-in-a-vesicle with asymmetric lipid membranes, and cell-sized asymmetric lipid vesicles using a fusion between nano-sized lipid vesicles and a planar lipid bilayer.

Academic Significance and Societal Importance of the Research Achievements

ナノサイズからマイクロサイズのリン脂質非対称膜リポソームの作製を成功している。このリン脂質非対称膜リポソームを用いて、リン脂質の非対称の生命現象への関与が詳細に観察できると考える。また、ナノサイズリポソームは、新しいドラッグデリバリーシステムの担体として利用可能である。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (32 results)

All 2021 2020 2019 2018 Other

All Journal Article (10 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 9 results,  Open Access: 4 results) Presentation (20 results) (of which Int'l Joint Research: 5 results,  Invited: 8 results) Remarks (2 results)

  • [Journal Article] Formation of nano-sized lipid vesicles with asymmetric lipid components using a pulsed-jet flow method2021

    • Author(s)
      Kamiya Koki、Osaki Toshihisa、Takeuchi Shoji
    • Journal Title

      Sensors and Actuators B: Chemical

      Volume: 327 Pages: 128917-128917

    • DOI

      10.1016/j.snb.2020.128917

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Investigation of Fusion between Nanosized Lipid Vesicles and a Lipid Monolayer Toward Formation of Giant Lipid Vesicles with Various Kinds of Biomolecules2021

    • Author(s)
      Kamiya Koki、Arisaka Chika、Suzuki Masato
    • Journal Title

      Micromachines

      Volume: 12 Issue: 2 Pages: 133-133

    • DOI

      10.3390/mi12020133

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of Artificial Cell Models Using Microfluidic Technology and Synthetic Biology2020

    • Author(s)
      Kamiya Koki
    • Journal Title

      Micromachines

      Volume: 11 Issue: 6 Pages: 559-559

    • DOI

      10.3390/mi11060559

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular and functional analysis of pore-forming toxin Monalysin from entomopathogenic bacterium Pseudomonas entomophila2020

    • Author(s)
      2.Nonaka S†, Salim E†, Kamiya K*, Hori A, Nainu F, Asri RM, Masyita A, Nishiuchii T, Takeuchi S, Kodera N*, Kuraishi T*
    • Journal Title

      Front. Immunol.

      Volume: 11 Pages: 520-520

    • DOI

      10.3389/fimmu.2020.00520

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Formation of vesicles-in-a-vesicle with asymmetric lipid components using a pulsed-jet flow method2019

    • Author(s)
      Kamiya Koki、Osaki Toshihisa、Takeuchi Shoji
    • Journal Title

      RSC Advances

      Volume: 9 Issue: 52 Pages: 30071-30075

    • DOI

      10.1039/c9ra04622d

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Automatic generation system of cell-sized liposomes2019

    • Author(s)
      Gotanda Masahide、Kamiya Koki、Osaki Toshihisa、Miki Norihisa、Takeuchi Shoji
    • Journal Title

      Sensors and Actuators B: Chemical

      Volume: 292 Pages: 57-63

    • DOI

      10.1016/j.snb.2019.04.096

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] リン脂質非対称膜リポソームの形成と機能観察2019

    • Author(s)
      神谷厚輝
    • Journal Title

      化学と工業

      Volume: 72 Pages: 1049-1050

    • Related Report
      2019 Annual Research Report
  • [Journal Article] 特集 生体膜のバイオロジー Ⅴ.新技術 マイクロデバイスを利用した人工脂質二重膜によるタンパク質機能解析2018

    • Author(s)
      神谷 厚輝、竹内 昌治
    • Journal Title

      生体の科学

      Volume: 69 Issue: 3 Pages: 252-257

    • DOI

      10.11477/mf.2425200805

    • ISSN
      0370-9531, 1883-5503
    • Year and Date
      2018-06-15
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Asymmetric lipid artificial membrane formation and biomolecule interaction investigation2018

    • Author(s)
      神谷 厚輝、竹内 昌治
    • Journal Title

      生化学

      Volume: 90 Issue: 2 Pages: 225-229

    • DOI

      10.14952/SEIKAGAKU.2018.900225

    • NAID

      40021617559

    • ISSN
      0037-1017
    • Year and Date
      2018-04-25
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Engineering of Cell-sized Liposomes2018

    • Author(s)
      KAMIYA Koki、TAKEUCHI Shoji
    • Journal Title

      Seibutsu Butsuri

      Volume: 58 Issue: 6 Pages: 291-296

    • DOI

      10.2142/biophys.58.291

    • NAID

      130007521711

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] Observation of GM1 flip-flop on cell-sized asymmetric GM1 liposomes prepared by nano-sized liposome fusion method2021

    • Author(s)
      Msato Suzuki, Koki Kamiya
    • Organizer
      65th Biophysical Society Annual Meeting
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 両親媒性タンパク質を用いたリン脂質-タンパク質小胞の構築2021

    • Author(s)
      鈴木允人、神谷厚輝
    • Organizer
      日本化学会 第101春季年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ナノサイズリポソーム融合による細胞サイズGM1非対称膜リポソームの構築2020

    • Author(s)
      鈴木允人、神谷厚輝
    • Organizer
      第58回日本生物物理学会 年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ナノサイズリポソーム融合により作製したGM1非対称膜リポソームにおけるフリップ・フロップの観察2020

    • Author(s)
      鈴木允人、神谷厚輝
    • Organizer
      細胞を創る研究会13.0
    • Related Report
      2020 Annual Research Report
  • [Presentation] 複雑な生命現象の理解に向けた高次な人工細胞膜の創成2020

    • Author(s)
      神谷厚輝
    • Organizer
      細胞を創る研究会13.0
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Asymmetric lipid vesicle generation for creating complex artificial cells2019

    • Author(s)
      Koki Kamiya
    • Organizer
      MARSS2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Asymmetric Lipid Vesicle Formation Using Microfluidic Technologies2019

    • Author(s)
      Koki Kamiya
    • Organizer
      he 3rd Joint-Symposium on Chemistry between Xiamen University and Gunma University
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] マイクロデバイスによる細胞膜模倣型人工細胞膜の創成と機能観察2019

    • Author(s)
      神谷厚輝
    • Organizer
      化学とマイクロ・ナノシステム学会 第39回研究会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 複雑な生命現象の理解に向けた細胞膜模倣型人工細胞膜の創成2019

    • Author(s)
      神谷厚輝
    • Organizer
      群馬大学第26回生命科学セミナー
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 真核細胞の細胞膜を模倣した人工細胞膜の創成2019

    • Author(s)
      神谷厚輝
    • Organizer
      第92回日本生化学会大会 シンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Insertion Measurement of OmpLA Protein into Lipid Bilayer using Ion Current Measurement Device2019

    • Author(s)
      Seren Ohnishi and Koki Kamiya
    • Organizer
      MicroTAS2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] リン脂質非対称膜小胞封入リポソームによる小胞の膜融合観察2019

    • Author(s)
      神谷厚輝、大崎寿久、竹内昌治
    • Organizer
      「細胞を創る」研究会12.0
    • Related Report
      2019 Annual Research Report
  • [Presentation] 脂質組成によるナノポア形成タンパク質の再構成挙動の検討2019

    • Author(s)
      大西瀬蓮、神谷厚輝
    • Organizer
      「細胞を創る」研究会12.0
    • Related Report
      2019 Annual Research Report
  • [Presentation] 脂質電荷による人工膜へのポアタンパク質再構成の制御2019

    • Author(s)
      大西瀬蓮、神谷厚輝
    • Organizer
      日本化学会関東支部群馬地区研究交流会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 細胞膜環境を模倣した人工細胞膜の創出2019

    • Author(s)
      神谷厚輝
    • Organizer
      日本化学会 第99春季年会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Automatic Planar Asymmetric Lipid Bilayer Membrane Formation Toward Biological High-Throughput Assay2018

    • Author(s)
      Masahide Gotanda, Koki Kamiya, Toshihisa Osaki, Satoshi Fujii, Nobuo Misawa, Norihisa Miki, and Shoji Takeuchi
    • Organizer
      40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society(EMBC’18)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Formation of giant vesicle containing small vesicles with asymmetric lipid membranes2018

    • Author(s)
      Koki Kamiya, Toshihisa Osaki, Shoji Takeuchi
    • Organizer
      第56回日本生物物理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 人工細胞膜によるイオンチャネル計測とその応用2018

    • Author(s)
      神谷厚輝
    • Organizer
      Nanion・東京女子医大イオンチャネルフォーラム2018
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 小胞輸送を模倣したリン脂質非対称膜の融合観察2018

    • Author(s)
      神谷厚輝、大崎寿久、竹内昌治
    • Organizer
      化学とマイクロ・ナノシステム学会第38 回研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 速度制御された液滴 接触により形成される脂質二重膜の評価2018

    • Author(s)
      五反田真秀、神谷厚輝、大崎寿久、三木則尚、竹内昌治
    • Organizer
      第 9 回マイクロ・ナノ工学シンポジ ウム
    • Related Report
      2018 Annual Research Report
  • [Remarks] 群馬大学 大学院理工学府 分子科学部門 神谷研究室

    • URL

      http://kamiya.chem-bio.st.gunma-u.ac.jp/index2.html

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
  • [Remarks] 群馬大学 大学院理工学府 分子科学部門 神谷研究室

    • URL

      http://kamiya.chem-bio.st.gunma-u.ac.jp/index2.html

    • Related Report
      2018 Annual Research Report

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

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