Function and dynamic regulation of animal specific RNA modifications
Project/Area Number |
18H02094
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Review Section |
Basic Section 37020:Chemistry and chemical methodology of biomolecules-related
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Research Institution | The University of Tokyo |
Principal Investigator |
Suzuki Takeo 東京大学, 大学院工学系研究科(工学部), 講師 (90533125)
|
Project Period (FY) |
2018-04-01 – 2022-03-31
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Project Status |
Completed (Fiscal Year 2021)
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Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2021: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2020: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
|
Keywords | tRNA / 生合成 / デコーディング / RNA修飾 / アンチコドン / RNA / 修飾 / ミトコンドリア |
Outline of Final Research Achievements |
We determined modifying genes of queuosine derivatives found in the wobble position of some animal tRNAs. Knocking out each gene in cultured cells revealed the elevation of protein aggregates in cytoplasm, suggesting that the hypomodification of the modification by gene KO altered decoding ability of corresponding tRNA. To deeply understand the molecular and physiological function of these modifications, further investigations will be required.
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Academic Significance and Societal Importance of the Research Achievements |
RNA修飾のヒトや動物個体における機能は詳細が明らかでないものが多く、今回、修飾酵素の新たな特定を通じて、修飾機能の解明に迫った。特にコドン解読という、あらゆる生命に共通するプロセスにおいて当該修飾の特徴的な役割を提唱したことは一定の意義がある。また今回特定した遺伝子の中に、遺伝子異常が神経変性疾患と相関する例を見出しているため、病態の理解や疾患の診断や治療への応用が期待される。
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Complete chemical structures of human mitochondrial tRNAs2020
Author(s)
Suzuki Takeo、Yashiro Yuka、Kikuchi Ittoku、Ishigami Yuma、Saito Hironori、Matsuzawa Ikuya、Okada Shunpei、Mito Mari、Iwasaki Shintaro、Ma Ding、Zhao Xuewei、Asano Kana、Lin Huan、Kirino Yohei、Sakaguchi Yuriko、Suzuki Tsutomu
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Journal Title
Nature Communications
Volume: 11
Issue: 1
Pages: 1-15
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Mammalian NSUN2 introduces 5-methylcytidines into mitochondrial tRNAs2019
Author(s)
Shinoda, S., Kitagawa, S., Nakagawa, S., Wei, F. Y., Tomizawa, K., Araki, K., Araki, M., Suzuki, T., Suzuki, T.
Organizer
21st RNA meeting
Related Report
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[Presentation] Cap-specific terminal N6-methylation of RNA by an RNA polymerase II-associated methyltransferase2019
Author(s)
Akichika, S., Hirano, S., Shichino, Y., Suzuki, T., Nishimasu, H., Ishitani, R., Sugita, A., Hirose, Y., Iwasaki, S., Nureki, O., Suzuki, T.
Organizer
20th Takeda Science Foundation Symposium on Bioscience, RNA Neobiology
Related Report
Int'l Joint Research
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[Presentation] ヒト疾患に関わるtRNAタウリン修飾の生合成と代謝制御2018
Author(s)
Suzuki, T., Asano, K., Saito, A., Wei, F. Y., Tomizawa, K., Sakaguchi, Y., Suzuki, T.
Organizer
20th RNA meeting
Related Report
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