| Project/Area Number |
18H02125
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 38020:Applied microbiology-related
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
國武 絵美 三重大学, 生物資源学研究科, 助教 (30800586)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
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| Keywords | カーボンカタボライト抑制 / Aspergillus nidulans / 多糖分解酵素 / 転写制御 / 糸状菌 / 多糖分解酵素遺伝子 / 生産制御 / cAMPシグナリング / パラログ間競合 / 情報伝達系 |
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| Outline of Final Research Achievements |
Production of polysaccharide degrading enzymes in filamentous fungi is generally under regulation of carbon catabolite repression (CCR), however, sensitivity to CCR differs depending on the enzyme type. Strength of CCR also differs depending on the repressing carbon source. Thus, the production is finely regulated. CCR is mediated by the transcription factor CreA as well as by PkaA and GanB in Aspergillus nidulans. The deubiquitinase CreB is also required for CCR. To further understand mechanisms of CCR, we compared the effects of creA deletion with deletion of pkaA and ganB genes as well as creB. This study revealed that PkaA and GanB participate in CreA-independent CCR and that contribution of CreA, PkaA, and GanB in CCR differs depending on the inducers, repressing carbon sources, the enzyme types and culture conditions. It also revealed that CreB functions independently of GanB. In addition, we identified a promising candidate of PkaA substrate that regulates CCR.
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| Academic Significance and Societal Importance of the Research Achievements |
糸状菌由来の多糖分解酵素は市場性の高い有用酵素であり、食品、繊維などの産業で幅広く利用されている。さらに、バイオマス有効利用の観点から近年需要が高まっている。そこで酵素価格を低く抑える必要があるが、そのネックとなる原因の一つがカーボンカタボライト抑制による生産量の低下である。本研究では複数の独立した経路がCCRに関わることを示し、多重破壊株を用いた効率的酵素生産法確率のための基盤情報を提供した。
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