Elucidation of the mechanism for controlling of the uric acid synthesis pathway by miRNA
| Project/Area Number |
18H02212
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 39050:Insect science-related
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
Tabuonki Hiroko 東京農工大学, (連合)農学研究科(研究院), 教授 (80434190)
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| Co-Investigator(Kenkyū-buntansha) |
佐藤 令一 東京農工大学, (連合)農学研究科(研究院), 教授 (30235428)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2020: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
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| Keywords | miRNA / Dicer / ステムループ構造 / 尿酸 / 抗酸化酵素 / JH / DJ-1 / 尿酸合成 / Bombyx mori / カイコ |
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| Outline of Final Research Achievements |
In this study, we analyzed the effects of antioxidative enzymes on the uric acid synthesis pathway using silkworm B.mori through functional analysis of BMmirmegu. As a result, the expression of BMmirmegu was high 96 hours after embryogenesis through the developmental stage. Next, we analyzed the relationship between JH and BMmirmegu. We found that injection of mimic for BMmirmegu into the larvae tended to increase the expression of the JH responsive gene and decrease the uric acid synthesis gene expression. Injection of JH into the larvae increased the expression of BMmirmegu. Therefore, we were clarified that BMmirmegu is a miRNA having a function similar to JH.
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| Academic Significance and Societal Importance of the Research Achievements |
カイコの幼虫の皮膚は尿酸が蓄積するため白い。加えてカイコには皮膚への尿酸蓄積量が低下した"白くない"カイコも多数存在する。カイコのように尿酸の解析に特化できる優れた生物は存在しない。本研究では、"白くない"カイコからステムループ構造が変異したBMmirmeguを見出し、その機能解析を通し、これまで未知であったステムループ構造の役割の理解を深化させた。これらの成果は論文、学会発表、プレスリリース等を通して広く社会に学術的意義を伝えた。
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Report
(2 results)
Research Products
(16 results)
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[Journal Article] Analysis of molecular mechanism for acceleration of polyembryony using gene functional annotation pipeline in Copidosoma floridanum.2020
Author(s)
Sakamoto, T., Nishiko, M., Bono, H., Nakazato, T., Yoshimura, J., Tabunoki, H., Iwabuchi, K.
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Journal Title
BMC Genomics
Volume: 21
Issue: 1
Pages: 152-152
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Apoptosis-mediated vasa down-regulation controls developmental transformation in Japanese Copidosoma floridanum female soldiers.2019
Author(s)
Ohno,H., Sakamoto,T., Okochi, R., Nishiko, M., Sasaki, S., Bono, H., Tabunoki, H., Iwabuchi, K.
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Journal Title
Developmental Biology
Volume: 456
Issue: 2
Pages: 226-233
DOI
Related Report
Peer Reviewed
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